Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 182
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | PE |
| Peptide Sequence | WMHHNMDLI |
| Citations for H-2Db HY Uty Tetramer-WMHHNMDLI-PE – 2 Found |
| Yamada, Hisakata; Shibata, Kensuke; Sakuraba, Koji; Fujimura, Kenjiro; Yoshikai, Yasunobu. Positive selection of self-antigen-specific CD8+ T cells by hematopoietic cells. European Journal Of Immunology. 2013;43(8):2033-42. PubMed |
| Cheung, Kenneth; Ma, Liang; Wang, Guosu; Coe, David; Ferro, Riccardo; Falasca, Marco; Buckley, Christopher D; Mauro, Claudio; Marelli-Berg, Federica M. CD31 signals confer immune privilege to the vascular endothelium. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2015;112(43):E5815-24. PubMed |
