A complete reagent set to measure 2DG in cells after cell lysis by sonication
Insulin is a hormone that lowers the blood glucose level and is known to help glucose enter cells through an effect that enhances the activity of glucose transport, primarily in insulin-sensitive cells, such as adipocytes and skeletalmuscles.
The effect of insulin and other growth factors on cells is often assessed in the laboratory by measuring glucose uptake activity. Typically, glucose uptake is measured using 3-O-Methyl-D-Glucose or 2-deoxyglucose (2DG) labeled with radioactive 3H or other isotopes. However, the use of radioactive isotopes is not available to all labs and is subject to many restrictions. This assay kit was developed to provide a simple, rapid and convenient means to measure cellular glucose uptakewithout the use of radioisotope.
The effect of insulin and other growth factors on cells is often assessed in the laboratory by measuring glucose uptake activity. Typically, glucose uptake is measured using 3-O-Methyl-D-Glucose or 2-deoxyglucose (2DG) labeled with radioactive 3H or other isotopes. However, the use of radioactive isotopes is not available to all labs and is subject to many restrictions. This assay kit was developed to provide a simple, rapid and convenient means to measure cellular glucose uptakewithout the use of radioisotope.
| Documents & Links for Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) | |
| Datasheet | csr-mbr-pmg-k01e_glucose-cellular-uptake-measurement-kit-broad-range-fluorometric_datasheet.pdf |
| Vendor Page | Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) at Cosmo Bio LTD |
| Documents & Links for Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) | |
| Datasheet | csr-mbr-pmg-k01e_glucose-cellular-uptake-measurement-kit-broad-range-fluorometric_datasheet.pdf |
| Vendor Page | Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) |
| Citations for Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) – 1 Found |
| Aoi, Wataru; Hirano, Nariyuki; Lassiter, David G; Björnholm, Marie; Chibalin, Alexander V; Sakuma, Kunihiro; Tanimura, Yuko; Mizushima, Katsura; Takagi, Tomohisa; Naito, Yuji; Zierath, Juleen R; Krook, Anna. Secreted protein acidic and rich in cysteine (SPARC) improves glucose tolerance via AMP-activated protein kinase activation. Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology. 2019;33(9):10551-10562. PubMed |