CARD FERTIUP® Cryoprotectant: CPA is a mouse sperm cryoprotectant that guards against damage to spermatozoa during cryopreservation. This reagent significantly improves in vitro fertilization rates of frozen and thawed sperm even with notoriously IVF-inefficient mouse strains such as C57BL/6 and 129. Use it to preserve and protect your valuable mouse resources.
Sperm cryopreservation is a practical and cost-effective method of archiving laboratory mouse strains. FERTIUP® series products and CARD MEDIUM are high-performance media for mouse sperm cryopreservation, sperm preincubation and IVF. When used in combination, FERTIUP® and CARD MEDIUM provide a high and consistent rate of fertilization from frozen-thawed mouse sperm, which will allow you to efficiently recover mouse lines when you need them.
CARD FERTIUP® Cryoprotectant: CPA improves sperm survival after thawing and subsequent in vitro fertilization. Sperm can be cryopreserved with high survival rate even with the C57BL/6 and 129 lines, which were said to have low survival rates with conventional sperm freezing. Storage of genetic resources with frozen sperm is simpler than the method of storing fertilized eggs and can be reduced to 1/30 or less compared to the cost of live maintenance of stock. It is also ideal as a preparation for system disconnection, natural disasters and unexpected accidents.
For information on techniques for mouse spermatozoa cryopreservation, spermatozoa thawing, in vitro fertilization and obtaining pups through embryo transfer, please refer to the CARD website: http://card.medic.kumamoto-u.ac.jp/card/english/sigen/index.html
| Documents & Links for CARD FERTIUP Cryoprotectant: CPA | |
| Datasheet | CARD FERTIUP Cryoprotectant: CPA Datasheet |
| Manual | kyd-001-ex_card-fertiup-cryoprotectant-cpa_manual_57294.pdf |
| Documents & Links for CARD FERTIUP Cryoprotectant: CPA | |
| Datasheet | CARD FERTIUP Cryoprotectant: CPA Datasheet |
| Manual | kyd-001-ex_card-fertiup-cryoprotectant-cpa_manual_57294.pdf |
| Citations for CARD FERTIUP Cryoprotectant: CPA – 1 Found |
| Takeo T., Hoshii T., Kondo Y., Toyodome H., Arima H., Yamamura KI., Irie T., and Nakagata N. et al. 2008. Methyl-betacyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. . Biol Reprod.. 78(3):546-51. |
