Exorapid-qIC(R) Immunochromatographic kit for extracellular vesicles (CD63)

Cosmo Bio

Catalog No: DNT-EXO-K02

 "Exorapid-qIC - Immunochromatography Kit for Extracellular Vesicles" is an immunochromatography kit which can detect extracellular vesicles (EVs) such as exosomes, microvesicles, apoptotic bodies, and largeonconsomes. It is developed by DNT and Shimadzu Corporation, making use of DNT's metal nanoparticles "Gold Nanoplates".

EVs are substances (endoplasmic reticulum) secreted from cells, and are abundant in blood and urine. Since they contain various information derived from the secreting cells, they are attracting attention as a research subject in the field of bioresearch.

For example, EVs secreted from cancer cells (malignant tumors) contain information derived from the secreting cancer cells, so the detection of EVs is expected to lead to early cancer diagnosis. In addition to diagnostic applications, EVs have also been attracting attention in the beauty and medical fields in recent years. In this way, EVs are expected to be used as diagnostic markers*1 and medical materials.

However, many of the analytical methods currently used in EVs research have issues, such as complicated processes or the need for expensive analytical equipment.

We have focused on immunochromatography (IC)*2, which is used in diagnostic kits for influenza viruses and the new coronavirus, as a quick and easy testing method. We have commercialized this kit through joint development with Shimadzu Corporation, which has a high level of technology and track record in the fields of measuring instruments and medical equipment.

*1. Components that are tested when diagnosing diseases.
*2. A method in which a sample solution flows over a cellulose membrane due to capillary action, and the presence or absence of a sample is determined by the presence or absence of coloration. Basically, no dedicated equipment is required.

Product features

• No dedicated equipment required! Testing completed in about 45 minutes!
• Ideal for starting up EVs research and simple evaluation
• Three types of kits allow EVs detection in various samples!

Applications
Evaluation of cell activity
- Confirmation of the amount of EVs secreted from cells
- Back data of EVs used in experiments
Quantification and management of EVs amount
- Measurement of EVs amount in samples (for evaluation of culture medium and secretion promoters, etc.)
- Confirmation of change in amount over time
Comparison of CD9, CD63, and CD81 protein amounts
- Comparison of amounts between samples

Contents for the following kits

• Exorapid-qIC® Immunochromatography Kit for Extracellular Vesicles (CD9)
• Exorapid-qIC® Immunochromatography Kit for Extracellular Vesicles (CD63)
• Exorapid-qIC® Immunochromatography Kit for Extracellular Vesicles (CD81)

① Immunochromatographic test strips...40 sheets
② Gold nanoplate labeled antibody [freeze-dried product]...1 bottle
③ Standard substance [freeze-dried product]...1 bottle
④ Sample dilution solution...1 bottle
⑤ Cleaning solution...1 bottle
⑥ Assay microplate 96 well...1 sheet
➆ Instruction manual.

Contents for the following set kit

• Exorapid-qIC® Immunochromatography Kit for Extracellular Vesicles (CD9, CD63, CD81 set)

① Immunochromatographic test strips...6 sheets each (total 18 sheets)
② Gold nanoplate labeled antibody [freeze-dried product]...1 bottle each (total of 3 bottles)
③ Standard substance [freeze-dried product]...1 bottle each (total 3 bottles)
④ Sample dilution solution...1 bottle
⑤ Cleaning solution...1 bottle
⑥ Assay microplate 96 well...1 sheet
➆ Instruction manual.