JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA and the production of ssDNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.
Features
- Rapid growing strain
- Carries F’ episome
Applications
- Preparation of ssDNA
- Construction of gene library
- Subcloning
Genotype
e14–(McrA–) recA1 endA1 gyrA96 thi-1 hsdR17(rK– mK+) supE44 relA1 D(lac–proAB) [F’ traD36 proAB lacIqZDM15]
Specifications
- Efficiency >1 x 10^7 cfu/μg
- 100 μl/vial (10 or 80 vials)
- Control Plasmid: (pUC19)
| Documents & Links for ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg) | |
| Manual | ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg) Manual |
| Vendor Page | ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg) at Yeastern |
| Documents & Links for ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg) | |
| Manual | ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg) Manual |
| Vendor Page | ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg) |