This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Features
- lack of Lon and Omp proteases allows high-level protein expression and accumulation
- Easy gene induction
Applications
- IPTG induced gene expression in E. coli
- Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
- Subcloning
Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Specifications
- Efficiency >2 x 10^7 cfu/μg
- 100 μl/vial (10 or 40 vials)
- Control Plasmid: (pUC19)
| Documents & Links for ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) | |
| Manual | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) Manual |
| Vendor Page | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) at Yeastern |
| Documents & Links for ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) | |
| Manual | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) Manual |
| Vendor Page | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) |