DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. The complete genome sequence has been published.
Features
- Streptomycin resistant
- High transformation efficiency
Applications
- Suitable for genomic DNA cloning
- Allow large inserts
- Cloning of methylated cytosine or adenine containing DNA
- Blue/white screening
Genotype
F–endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ–
Specifications
- Efficiency >5 x 10^7 cfu/μg
- 100 μl/vial (10 or 80 vials)
- Control Plasmid: (pUC19)
| Documents & Links for ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg) | |
| Manual | ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg) Manual |
| Vendor Page | ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg) at Yeastern |
| Documents & Links for ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg) | |
| Manual | ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg) Manual |
| Vendor Page | ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg) |