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Background
This Diphtheria toxin CRM197 was highly purified from growth media of Corynebacterium
diphtheriae mutant CRM197. CRM197 like wild-typediphtheria toxin is a single polypeptide
chain of 535 amino acids (58 kD) consisting of two subunits linked by disulfide bridges. Binding
to the cell surface of the less stable of these two subunits allows the more stable part of the
protein to penetrate the host cell. Wild-type toxin catalyzes the ADP-ribosylation of eucaryotic
elongation factor-2 (eEF2) by using NAD, thus inactivating this protein. However, CRM197 has
an alteration of 52nd Gly to Glu and it has no ADP ribosylation activity nor toxicity to cells.
Specification
Package size: 200 Pg
Purity: More than 95% pure (see below; SDS-PAGE without mercaptoethanol)
Form: 2 mg/ml in 20 mM Tris-Hcl (pH 7.2), 150 mM NaCl
Storage: - 70C
Application
1) CRM197 retains activity to bind the receptor, HB-EGF
(Heparin-Binding EGF-like Growth Factor) and inhibits the growth-stimulating
activity of HB-EGF.
2) Putative drug for treatment of malignant tumors such as ovarian tumor,
which secretes higher levels of HB-EGF.
Documents & Links for Diphtheria Toxin Mutant CRM197 | |
Datasheet | Diphtheria toxin mutant CRM197 Datasheet |
Vendor Page | Diphtheria Toxin Mutant CRM197 at Cosmo Bio LTD |
Documents & Links for Diphtheria Toxin Mutant CRM197 | |
Datasheet | Diphtheria toxin mutant CRM197 Datasheet |
Vendor Page | Diphtheria Toxin Mutant CRM197 |