DCNL1 [GST-tagged]

Ubiquigent

Catalog No: UBI-63-2001-025

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Species Reactivity: human  

Source: E. coli  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: 57.1kDa  

Protein Sequence: Accession number: NP_65691.2 For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E3 Ligase Assay: The activity of GST-DCNL1 was validated through its ability to enhance the neddylation of Cul1/Rbx1/Skp1 acting as a substrate in the presence of the thioester-loaded His-Ube2M~NEDD8. Incubation of Cul1/Rbx1/Skp1 and thioester loaded His-Ube2M~NEDD8 in the presence or absence of GST-DCNL1 at 4 °C was compared at two time points T0 and T2 minutes. Increased neddylation of the Cul1 subunit in the presence of GST-DCNL1 was demonstrated.