CycLex® SIRT1/Sir2 DeacetylaseFluorometric Assay Kit Ver.2

MBL

Catalog No: MBL-CY-1151V2

The CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit detects SIRT1/Sir2 activity in lysates. Primarily, the CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of SIRT1/Sir2 inhibitors or activators using crude SIRT1/Sir2 fraction or purified SIRT1/Sir2. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT1/Sir2 activity with the CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit if the appropriate antibody direct against SIRT1 or Sir2 is used for immunoprecipitation.

Citations for CycLex® SIRT1/Sir2 DeacetylaseFluorometric Assay Kit Ver.2 – 9 Found

Kayashima, Yasunari; Katayanagi, Yuki; Tanaka, Keiko; Fukutomi, Ryuta; Hiramoto, Shigeru; Imai, Shinjiro. Alkylresorcinols activate SIRT1 and delay ageing in Drosophila melanogaster. Scientific Reports. 2017;7:43679. 

Peng, Jing; Li, Qingde; Li, Keye; Zhu, Li; Lin, Xiaoding; Lin, Xiaohong; Shen, Qianhui; Li, Guoping; Xie, Xi. Quercetin Improves Glucose and Lipid Metabolism of Diabetic Rats: Involvement of Akt Signaling and SIRT1. Journal Of Diabetes Research. 2017:3417306. 

Conti, Valeria; Corbi, Graziamaria; Manzo, Valentina; Malangone, Paola; Vitale, Carolina; Maglio, Angelantonio; Cotugno, Roberta; Capaccio, Damiano; Marino, Luigi; Selleri, Carmine; Stellato, Cristiana; Filippelli, Amelia; Vatrella, Alessandro. SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease. Oxidative Medicine And Cellular Longevity. 2018:9391261. 

Shigemoto, Ryosuke; Matsumoto, Takara; Masuo, Shunsuke; Takaya, Naoki. 5-Methylmellein is a novel inhibitor of fungal sirtuin and modulates fungal secondary metabolite production. The Journal Of General And Applied Microbiology. 2018;64(5):240-247. 

Corbi, G; Conti, V; Komici, K; Manzo, V; Filippelli, A; Palazzo, M; Vizzari, F; Davinelli, S; Di Costanzo, A; Scapagnini, G; Ferrara, N; Casamassima, D. Phenolic Plant Extracts Induce Sirt1 Activity and Increase Antioxidant Levels in the Rabbit's Heart and Liver. Oxidative Medicine And Cellular Longevity. 2018:2731289. 

Xu, Siqi; Zeng, Zhenhua; Zhao, Ming; Huang, Qiaobing; Gao, Youguang; Dai, Xingui; Lu, Jiayin; Huang, Weiqing; Zhao, Keseng. Evidence for SIRT1 Mediated HMGB1 Release From Kidney Cells in the Early Stages of Hemorrhagic Shock. Frontiers In Physiology. 10:854. 
- Comments: Used in: ELISA

Yaku, Keisuke; Okabe, Keisuke; Gulshan, Maryam; Takatsu, Kiyoshi; Okamoto, Hiroshi; Nakagawa, Takashi. Metabolism and biochemical properties of nicotinamide adenine dinucleotide (NAD) analogs, nicotinamide guanine dinucleotide (NGD) and nicotinamide hypoxanthine dinucleotide (NHD). Scientific Reports. 2019;9(1):13102. 

Corbi, Graziamaria; Conti, Valeria; Troisi, Jacopo; Colucci, Angelo; Manzo, Valentina; Di Pietro, Paola; Calabrese, Maria Consiglia; Carrizzo, Albino; Vecchione, Carmine; Ferrara, Nicola; Filippelli, Amelia. Cardiac Rehabilitation Increases SIRT1 Activity and β-Hydroxybutyrate Levels and Decreases Oxidative Stress in Patients with HF with Preserved Ejection Fraction. Oxidative Medicine And Cellular Longevity. 2019:7049237. 

Chiang, Shannon; Kalinowski, Danuta S; Dharmasivam, Mahendiran; Braidy, Nady; Richardson, Des R; Huang, Michael L H. The potential of the novel NAD(+) supplementing agent, SNH6, as a therapeutic strategy for the treatment of Friedreich's ataxia. Pharmacological Research. 2020;155:104680.