Cul3/Rbx1 [untagged]

Ubiquigent

Catalog No: UBI-63-1003-025

Species Reactivity: human  

Source: Insect (Sf21)  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: Cul3= 89kDa; Rbx1=12kDa  

Protein Sequence: Accession number: Cul3=NP_003581.1; Rbx1=NP_055063.1. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E3 Ligase Assay: The activity of Cul3/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul3/Rbx1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL2 at 4 °C was compared at two time points T0 and T2 minutes. Neddylation of the Cul3 subunit in the presence of His-DCNL2 was demonstrated.