A cryopreservation solution for various cells such as human iPS / ES cells and CHO cells. Contains 5% DMSO. It does not contain animal-derived ingredients and protein ingredients.
For human iPS / ES cells, a high engraftment rate can be obtained by using it as a set with "Pronase / EDTA for Stem" as a stripping solution.
Storage method: 1 to 30 ° C
Validity period: 24 months
Purpose of use
Slow freexing of human iPS/ES cells
| Composition | % |
| Hydroxyethyl starch(HES) | 6% |
| Dimethyl sulfoxide(DMSO) | 5% |
| Ethylene glycol | 5% |
| Sodium chloride |
References
1) Imaizumi, Keitaro. et al, : A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. PLoS One. 2014 Feb 12;9(2):e88696.
2) Imaizumi, Keitaro. et al, : A simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells. ES Cells: Methods and Protocols-2nd Edition, Springer Protocols, 2015 in press.
| Documents & Links for CP-5E | |
| Datasheet | CP-5E Datasheet |
| Documents & Links for CP-5E | |
| Datasheet | CP-5E Datasheet |