Purpose of Use
For screening Enterohemorrhagic Escherichia coli O157:H7/-
CLIG Agar (cellobiose added LIG agar medium)
Characteristics
CLIG Agar enables estimation and confirmation of E. coli by confirmation of acid
production from lactose and cellobiose, indole and -glucuronidase production, and is
useful for screening for enterohemorrhagic Escherichia coli O157:H7/-.
Composition (1 L = 41.0 g)
- Casein peptone 7.5 g MUG 0.02 g
- Meat peptone 2.5 g Sodium chloride 5.0 g
- Lactose 1.0 g Phenol red 0.025 g
- Cellobiose 10.0 g Agar powder 14.9 g
- Tryptophan 0.1 g
- Purified water 1,000 ml pH 7.4s
Preparation method
41.0 g of this product is added to 1,000 mL of purified water, and dissolved by heating.
2.5 to 3.0 mL of this agar medium is poured into each small size of test tube, and
high-pressure sterilization is performed at 115 degrees for 15 minutes.
After sterilization, solidify it as semi-slant medium such as TSI agar medium etc.
Test method
Inoculate the test bacterial strains in the bottom part and on the slant part, and
incubate at 35 to 37 degrees for 18 to 24 hours.
Caution: Do not keep it with a tight stopper (cap) during culture.
Interpretation of results
| Enterohemorrhagic E. coli O157:H7/- |
Other E. coli | |
| Bottom part | Change to yellow | Change to yellow |
| Slant part | Change to red | Change to red |
| Cytochrome oxidase | Negative | Negative |
| Indole production *1) | Positive | Positive |
| Blue fluorescence under UV irradiation *2) | Negative | Positive |
*1)Testing by indole spot test *2) Ultraviolet at 365 nm
After confirming the above properties, conduct agglutination test using anti-O157
serum.
| Documents & Links for CLIG Agar Medium | |
| Datasheet | CLIG Agar Medium Datasheet |
| Documents & Links for CLIG Agar Medium | |
| Datasheet | CLIG Agar Medium Datasheet |