PCSK9 binds to the LDL receptor in vitro and in vitro. Human PCSK9 D374Y containing a C-terminal His-tag, expressed in HEK 293 cells. Binding capability to EGF-AB domain of LDL receptor was confirmed by using CircuLex PCSK9-LDLR in vitro Binding Assay Kit (CY-8150). The PCSK9 D374Y in culture medium might be used for LDLR degradation assay on HepG2 cell. Unused PCSK9 D374Y in culture medium should be stored at -70°C.
| Product Specifications | |
| Formulation | Supplied frozen in DMEM, 10 % FCS and appropriate antibiotics in sterile condition. |
| Citations for CircuLex PCSK9 D374Y in culture medium – 3 Found |
| Lambert, Gilles; Petrides, Francine; Chatelais, Mathias; Blom, Dirk J; Choque, Benjamin; Tabet, Fatiha; Wong, Gida; Rye, Kerry-Anne; Hooper, Amanda J; Burnett, John R; Barter, Philip J; Marais, A David. Elevated plasma PCSK9 level is equally detrimental for patients with nonfamilial hypercholesterolemia and heterozygous familial hypercholesterolemia, irrespective of low-density lipoprotein receptor defects. Journal Of The American College Of Cardiology. 2014;63(22):2365-73. PubMed |
| Thedrez, Aurélie; Sjouke, Barbara; Passard, Maxime; Prampart-Fauvet, Simon; Guédon, Alexis; Croyal, Mikael; Dallinga-Thie, Geesje; Peter, Jorge; Blom, Dirk; Ciccarese, Milco; Cefalù, Angelo B; Pisciotta, Livia; Santos, Raul D; Averna, Maurizio; Raal, Frederick; Pintus, Paolo; Cossu, Maria; Hovingh, Kees; Lambert, Gilles. Proprotein Convertase Subtilisin Kexin Type 9 Inhibition for Autosomal Recessive Hypercholesterolemia-Brief Report. Arteriosclerosis, Thrombosis, And Vascular Biology. 2016;36(8):1647-50. PubMed |
| Hyrina, Anastasia; Olmstead, Andrea D; Steven, Paul; Krajden, Mel; Tam, Edward; Jean, François. Treatment-Induced Viral Cure of Hepatitis C Virus-Infected Patients Involves a Dynamic Interplay among three Important Molecular Players in Lipid Homeostasis: Circulating microRNA (miR)-24, miR-223, and Proprotein Convertase Subtilisin/Kexin Type 9. Ebiomedicine. 2017;23:68-78. PubMed |