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Source: E.coli
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Concentration: 0.5mg/ml
Molecular Weight: ~97kDa
Protein Sequence: Accession number: NP_001157. For full protein sequence information download the Certificate of Analysis pdf.
Quality Control Protein Identification: Confirmed by mass spectrometry.
Quality Control Activity: E3 Ligase Assay: The ubiquitin conjugating activity of GST-BIRC2 was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme 6His-UBE1, the E2 conjugating enzyme His-UBE2D3 (UbcH5c) (several E2s were tested, data generated with this E2 is provided by way of example) and ubiquitin. Incubation of GST-BIRC2 for 30 minutes at 30oC in the presence of ubiquitin, 6His-Ube1, His-UBE2D3 and ATP (Lane 1) was compared alongside two control reactions with either ATP (Lane 2) or GST-BIRC2 (Lane 3) excluded from the reaction. Ubiquitin conjugates were identified by Western blotting using an anti-ubiquitin conjugate antibody and these were observed only in the presence of both ATP and GST-BIRC2.
Documents & Links for BIRC2 [GST-tagged] | |
Datasheet | ubi-63-0015-025_birc2-gst-tagged_datasheet.pdf |
Vendor Page | BIRC2 [GST-tagged] at Ubiquigent |
Documents & Links for BIRC2 [GST-tagged] | |
Datasheet | ubi-63-0015-025_birc2-gst-tagged_datasheet.pdf |
Vendor Page | BIRC2 [GST-tagged] |
Citations for BIRC2 [GST-tagged] – 1 Found |
Burge, Rebecca J; Jameson, Katie H; Wilkinson, Anthony J; Mottram, Jeremy C. In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay. Bio-Protocol. 2022;12(21) PubMed |