Human telomerase reverse transcriptase (hTERT) is 1132 amino acids, 127 kDa protein, that is the catalytic subunit of the telomerase. Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat unit, 5'-TTAGGG-3', to chromosome ends. Human telomerase consists of three major subunits: TERT, a template RNA called hTR, and telomerase-associated protein (TEP-1). hTERT and hTR are minimally required to reconstitute telomerase activity in vitro. In these subunits, hTR is constitutively expressed; TERT transcription is a primary mechanism for regulation of telomerase activity. In most human somatic cells, telomerase activity is undetectable, whereas approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity. Activation of telomerase is implicated in cell immortalization and cancer cell pathogenesis.
Monoclonal Antibody of 100 µl targeting TERT (Human) for WB,IP,ICC,ChIP.
| Product Specifications | |
| Application | ChIP, ICC, IP, WB |
| Reactivity | Human |
| Clonality | Monoclonal IgG2b ĸ |
| Host | Mouse |
| Gene ID - Human | |
| Formulation | 1 mg/mL in PBS/50% glycerol, pH 7.2 |
| Citations for Anti-TERT (Human) mAb (Clone: 10E9-2) – 6 Found |
| Maida, Yoshiko; Yasukawa, Mami; Okamoto, Naoko; Ohka, Seii; Kinoshita, Keita; Totoki, Yasushi; Ito, Takashi K; Minamino, Tohru; Nakamura, Hiromi; Yamaguchi, Satoko; Shibata, Tatsuhiro; Masutomi, Kenkichi. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Molecular And Cellular Biology. 2014;34(9):1576-93. PubMed - Comments: Used in: WB |
| Maida, Yoshiko; Yasukawa, Mami; Okamoto, Naoko; Ohka, Seii; Kinoshita, Keita; Totoki, Yasushi; Ito, Takashi K; Minamino, Tohru; Nakamura, Hiromi; Yamaguchi, Satoko; Shibata, Tatsuhiro; Masutomi, Kenkichi. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Molecular And Cellular Biology. 2014;34(9):1576-93. PubMed - Comments: Used in: IP |
| Maida, Yoshiko; Yasukawa, Mami; Okamoto, Naoko; Ohka, Seii; Kinoshita, Keita; Totoki, Yasushi; Ito, Takashi K; Minamino, Tohru; Nakamura, Hiromi; Yamaguchi, Satoko; Shibata, Tatsuhiro; Masutomi, Kenkichi. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Molecular And Cellular Biology. 2014;34(9):1576-93. PubMed - Comments: Used in: ICC |
| Maida, Yoshiko; Yasukawa, Mami; Okamoto, Naoko; Ohka, Seii; Kinoshita, Keita; Totoki, Yasushi; Ito, Takashi K; Minamino, Tohru; Nakamura, Hiromi; Yamaguchi, Satoko; Shibata, Tatsuhiro; Masutomi, Kenkichi. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Molecular And Cellular Biology. 2014;34(9):1576-93. PubMed - Comments: Used in: ChIP |
| Maida, Yoshiko; Yasukawa, Mami; Masutomi, Kenkichi. De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase. Molecular And Cellular Biology. 2016;36(8):1248-59. PubMed - Comments: Used in: IP |
| Takahashi, Masamichi; Miki, Shunichiro; Fujimoto, Kenji; Fukuoka, Kohei; Matsushita, Yuko; Maida, Yoshiko; Yasukawa, Mami; Hayashi, Mitsuhiro; Shinkyo, Raku; Kikuchi, Kiyomi; Mukasa, Akitake; Nishikawa, Ryo; Tamura, Kenji; Narita, Yoshitaka; Hamada, Akinobu; Masutomi, Kenkichi; Ichimura, Koichi. Eribulin penetrates brain tumor tissue and prolongs survival of mice harboring intracerebral glioblastoma xenografts. Cancer Science. 2019;110(7):2247-2257. PubMed - Comments: Used in: IP |