Figure 1. Immunoblot analysis with pAb pS410. Predicted molecular weight: Phosphorylated full-length TDP-43 at 45 kDa, non-phosphorylated TDP-43 at 43 kDa, - 25 kDa fragments and smearing substances in frontotemporal lobar degeneration (FTLD-U) and ALS.

Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb (Rabbit, Antiserum)

Catalog No:
CAC-TIP-PTD-P04
$524.00

Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKSS(p)GWGM].

This pAb recognizes human TDP-43 phosphorylated on serine 410 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.

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Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKSS(p)GWGM]. This pAb recognizes human TDP-43 phosphorylated on serine 410 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.

TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Specifications

  • Product type                primary antibody
  • Immunogen                 CMDSKSS(p)GWGM, S(p): phosphoserine 410
  • Raised in                     rabbit
  • Source                         serum
  • Form                           liquid antiserum with 0.05% NaN3 as a preservative
  • Volume                       100 uL
  • Label                           unlabeled
  • Specificity                   phospho-TDP-43
  • Cross reactivity           human
  • Storage                        below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.

Recommended dilutions

  • Western blotting: 1/1000-1/3000
  • Immunohistochemistry: 1/1000-1/5000
  • ELISA: 1/1000-1/5000
  • Other applications not tested
  • Optimal dilutions/concentrations should be determined by end user

References

  1. Hasegawa M, Arai T, Nonaka T, et al. Phosphorylated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Ann Neurol. 2008;64(1):60–70. doi:10.1002/ana.21425
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Documents & Links for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb (Rabbit, Antiserum)
Datasheet cac-tip-ptd-p04_anti-tdp-43-phospho-ser410-pab_datasheet.pdf

Documents & Links for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb (Rabbit, Antiserum)
Datasheet cac-tip-ptd-p04_anti-tdp-43-phospho-ser410-pab_datasheet.pdf