Figure 1. Immunoblot analysis with pAb pS409. Predicted molecular weight: Phosphorylated full-length TDP-43 at 45 kDa, non-phosphorylated TDP-43 at 43 kDa, - 25 kDa fragments and smearing substances in frontotemporal lobar degeneration (FTLD-U) and ALS.

Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb (Rabbit, Antiserum)

Catalog No:
CAC-TIP-PTD-P03
$680.00

Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKS(p)SGWGM].

This pAb recognizes human TDP-43 phosphorylated on serine 409 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.

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TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Specifications

  • Product type                primary antibody
  • Immunogen                 CMDSKS(p)SGWGM, S(p): phosphoserine 409
  • Raised in                     rabbit
  • Source                         serum
  • Form                           liquid antiserum with 0.05% NaN3 as a preservative
  • Volume                       100 uL
  • Label                           unlabeled
  • Specificity                   phospho-TDP-43
  • Cross reactivity           human
  • Storage                        below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.

Recommended dilutions

  • Western blotting                     1/1000-1/5000
  • Immunohistochemistry           1/1000-1/5000
  • ELISA                         1/1000-1/5000
  • Other applications not tested
  • Optimal dilutions/concentrations should be determined by end user.

References

  1. Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473
Documents & Links for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb (Rabbit, Antiserum)
Datasheet cac-tip-ptd-p03_anti-tdp-43-phospho-ser409-pab_datasheet.pdf

Documents & Links for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb (Rabbit, Antiserum)
Datasheet cac-tip-ptd-p03_anti-tdp-43-phospho-ser409-pab_datasheet.pdf

Citations for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb (Rabbit, Antiserum) – 1 Found
Nakazawa, Seshiru; Oikawa, Daisuke; Ishii, Ryohei; Ayaki, Takashi; Takahashi, Hirotaka; Takeda, Hiroyuki; Ishitani, Ryuichiro; Kamei, Kiyoko; Takeyoshi, Izumi; Kawakami, Hideshi; Iwai, Kazuhiro; Hatada, Izuho; Sawasaki, Tatsuya; Ito, Hidefumi; Nureki, Osamu; Tokunaga, Fuminori. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nature Communications. 2016;7( 27552911):12547.  PubMed