Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb (Rabbit, Antiserum)

Cosmo Bio

Catalog No: CAC-TIP-PTD-P03

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Product Description

TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Specifications

• Product type                primary antibody
• Immunogen                 CMDSKS(p)SGWGM, S(p): phosphoserine 409
• Raised in                     rabbit
• Source                         serum
• Form                           liquid antiserum with 0.05% NaN3 as a preservative
• Volume                       100 uL
• Label                           unlabeled
• Specificity                   phospho-TDP-43
• Cross reactivity           human
• Storage                        below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.

Recommended dilutions

• Western blotting                     1/1000-1/5000
• Immunohistochemistry           1/1000-1/5000
• ELISA                         1/1000-1/5000
• Other applications not tested
• Optimal dilutions/concentrations should be determined by end user.

References

1. Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473

Citations

Citations for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb (Rabbit, Antiserum) – 1 Found

Nakazawa, Seshiru; Oikawa, Daisuke; Ishii, Ryohei; Ayaki, Takashi; Takahashi, Hirotaka; Takeda, Hiroyuki; Ishitani, Ryuichiro; Kamei, Kiyoko; Takeyoshi, Izumi; Kawakami, Hideshi; Iwai, Kazuhiro; Hatada, Izuho; Sawasaki, Tatsuya; Ito, Hidefumi; Nureki, Osamu; Tokunaga, Fuminori. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nature Communications. 2016;7( 27552911):12547.