Anti TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb (Clone 11-9) (in PBS)

Cosmo Bio

Catalog No: CAC-TIP-PTD-M01A

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Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb 11-9 was cloned from mice immunized with diphospho-peptide [CMDSKS(p)S(p)GWGM]. 11-9 recognizes human TDP-43 phosphorylated on serines 409 and 410. 11-9 is validated for western blot, ELISA and IHC(f). This antibody is a powerful tool for biochemical and immunohistochemical analyses of TDP-43 proteinopathy.

TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases.

This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Cat. No. CAC-TIP-PDT-M01A Product Specifications

Product type	primary antibody
Immunogen	CMDSKS(p)S(p)GWGM, S(p): phosphoserine 409/410
Raised in	mouse
Clone name	11-9
Isotype	IgG1
Source	culture supernatant
Purification	affinity purified
Form	liquid containing PBS
Volume	100 uL
Label	unlabeled
Specificity	phospho-TDP-43
Cross reactivity	human
Storage	below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.

Important Information on Clone 11-9 Formulation History:

Currently shipping (since November 2022) CAC-TIP-PTD-M01A replaces discontinued CAC-TIP-PTD-M01.
-M01A (currently shipping; lot nos. 11-9-12-31-1 and newer): Affinity purified in PBS; Concentration: 1.0-1.5 mg/ml.
-M01 (lots 11-9-20 and 11-9-21): Ammonium sulfate precipitated; presented in 50% glycerol
-MO1 (prior to lot 11-9-20): provided as hybridoma culture supernatant.

Recommended dilutions:

• Western blotting                     1/1000-1/3000
• Immunohistochemistry           1/3000-1/10000
• ELISA                                     1/1000-1/5000
• Other applications not tested
• Optimal dilutions/concentrations should be determined by end user.

References

1. 1. Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473

Product Specifications

• Application: ELISA, IHC(f), WB
• Reactivity: Human
• Clonality: Monoclonal (Clone No.: 11-9)
• Host: Mouse

Citations for Anti TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb (Clone 11-9) (in PBS) – 3 Found

Murakami, Aya; Koga, Shunsuke; Sekiya, Hiroaki; Oskarsson, Björn; Boylan, Kevin; Petrucelli, Leonard; Josephs, Keith A; Dickson, Dennis W. Old age amyotrophic lateral sclerosis and limbic TDP-43 pathology. Brain Pathology (Zurich, Switzerland). 2022;32(6):e13100. 

Ko, Vivian I; Ong, Kailee; Cleveland, Don W; Yu, Haiyang; Ravits, John M. CK1δ/ε kinases regulate TDP-43 phosphorylation and are therapeutic targets for ALS-related TDP-43 hyperphosphorylation. Neurobiology Of Disease. 2024;196( 38677657):106516. 

Raulin, Ana-Caroline; Doss, Sydney V; Heckman, Michael G; Craver, Emily C; Li, Zonghua; Ikezu, Tadafumi C; Sekiya, Hiroaki; Liu, Chia-Chen; Martens, Yuka A; Rosenberg, Cassandra L; Kuchenbecker, Lindsey A; DeTure, Michael; Reichard, R Ross; Nguyen, Aivi T; Constantopoulos, Eleni; Larsen, Rachel A; Kounaves, Emmaline K; Murray, Melissa E; Dickson, Dennis W; Petersen, Ronald C; Bu, Guojun; Kanekiyo, Takahisa. Impact of APOE on amyloid and tau accumulation in argyrophilic grain disease and Alzheimer's disease. Acta Neuropathologica Communications. 2024;12(1):25.