SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, Sap 49, 130, 145, and 155. Sap155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. Sap155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. Sap155 contains numerous Cdk consensus phosphorylation sites in its N-terminus and is phosphorylated prior to catalytic step II of the splicing pathway. Sap155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.
This monoclonal antibody reacts with Sap155 (155 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry.
| Product Specifications | |
| Application | ICC, IP, WB |
| Reactivity | Human, Mouse |
| Clonality | Monoclonal IgG2b |
| Host | Mouse |
| Immunogen | Recombinant Sap155 (98-198 a.a.) |
| Formulation | 100 μg IgG in 100 μl PBS containing 50% glycerol, pH 7.2. Contains nopreservatives. |
| Citations for Anti-Sap155 mAb (Clone: 16) – 16 Found |
| Kotake, Yoshihiko; Sagane, Koji; Owa, Takashi; Mimori-Kiyosue, Yuko; Shimizu, Hajime; Uesugi, Mai; Ishihama, Yasushi; Iwata, Masao; Mizui, Yoshiharu. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nature Chemical Biology. 2007;3(9):570-5. PubMed - Comments: Used in: WB |
| Kotake, Yoshihiko; Sagane, Koji; Owa, Takashi; Mimori-Kiyosue, Yuko; Shimizu, Hajime; Uesugi, Mai; Ishihama, Yasushi; Iwata, Masao; Mizui, Yoshiharu. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nature Chemical Biology. 2007;3(9):570-5. PubMed - Comments: Used in: IP |
| Yamasaki, Sho; Ishikawa, Eri; Sakuma, Machie; Hara, Hiromitsu; Ogata, Koji; Saito, Takashi. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nature Immunology. 2008;9(10):1179-88. PubMed - Comments: Used in: WB |
| Tanuma, Nobuhiro; Kim, Sei-Eun; Beullens, Monique; Tsubaki, Yao; Mitsuhashi, Shinya; Nomura, Miyuki; Kawamura, Takeshi; Isono, Kyoichi; Koseki, Haruhiko; Sato, Masami; Bollen, Mathieu; Kikuchi, Kunimi; Shima, Hiroshi. Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155). The Journal Of Biological Chemistry. 2008;283(51):35805-14. PubMed - Comments: Used in: WB |
| Tanuma, Nobuhiro; Kim, Sei-Eun; Beullens, Monique; Tsubaki, Yao; Mitsuhashi, Shinya; Nomura, Miyuki; Kawamura, Takeshi; Isono, Kyoichi; Koseki, Haruhiko; Sato, Masami; Bollen, Mathieu; Kikuchi, Kunimi; Shima, Hiroshi. Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155). The Journal Of Biological Chemistry. 2008;283(51):35805-14. PubMed - Comments: Used in: IP |
| Yokoi, Akira; Kotake, Yoshihiko; Takahashi, Kentaro; Kadowaki, Tadashi; Matsumoto, Yoshiko; Minoshima, Yukinori; Sugi, Naoko H; Sagane, Koji; Hamaguchi, Makoto; Iwata, Masao; Mizui, Yoshiharu. Biological validation that SF3b is a target of the antitumor macrolide pladienolide. The Febs Journal. 2011;278(24):4870-80. PubMed - Comments: Used in: WB |
| Takemura, Reiko; Takeiwa, Toshihiko; Taniguchi, Ichiro; McCloskey, Asako; Ohno, Mutsuhito. Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells. Genes To Cells : Devoted To Molecular & Cellular Mechanisms. 2011;16(10):1035-49. PubMed - Comments: Used in: WB |
| Manceau, Valérie; Kremmer, Elisabeth; Nabel, Elizabeth G; Maucuer, Alexandre. The protein kinase KIS impacts gene expression during development and fear conditioning in adult mice. Plos One. 7(8):e43946. PubMed - Comments: Used in: WB |
| Kajiwara, Toshiko; Matsushita, Kazuyuki; Itoga, Sakae; Tamura, Mai; Tanaka, Nobuko; Tomonaga, Takeshi; Matsubara, Hisahiro; Shimada, Hideaki; Habara, Yasuaki; Matsuo, Masafumi; Nomura, Fumio. SAP155-mediated c-myc suppressor far-upstream element-binding protein-interacting repressor splicing variants are activated in colon cancer tissues. Cancer Science. 2013;104(2):149-56. PubMed - Comments: Used in: WB |
| Gao, Yang; Trivedi, Sumita; Ferris, Robert L; Koide, Kazunori. Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Scientific Reports. 2014;4:6098. PubMed - Comments: Used in: WB |
| Kfir, Nir; Lev-Maor, Galit; Glaich, Ohad; Alajem, Adi; Datta, Arnab; Sze, Siu K; Meshorer, Eran; Ast, Gil. SF3B1 association with chromatin determines splicing outcomes. Cell Reports. 2015;11(4):618-29. PubMed - Comments: Used in: WB |
| Kfir, Nir; Lev-Maor, Galit; Glaich, Ohad; Alajem, Adi; Datta, Arnab; Sze, Siu K; Meshorer, Eran; Ast, Gil. SF3B1 association with chromatin determines splicing outcomes. Cell Reports. 2015;11(4):618-29. PubMed - Comments: Used in: IP |
| Schreiber, Claire A; Sakuma, Toshie; Izumiya, Yoshihiro; Holditch, Sara J; Hickey, Raymond D; Bressin, Robert K; Basu, Upamanyu; Koide, Kazunori; Asokan, Aravind; Ikeda, Yasuhiro. An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses. Plos Pathogens. 2015;11(8):e1005082. PubMed - Comments: Used in: WB |
| Lee, Stanley Chun-Wei; Dvinge, Heidi; Kim, Eunhee; Cho, Hana; Micol, Jean-Baptiste; Chung, Young Rock; Durham, Benjamin H; Yoshimi, Akihide; Kim, Young Joon; Thomas, Michael; Lobry, Camille; Chen, Chun-Wei; Pastore, Alessandro; Taylor, Justin; Wang, Xujun; Krivtsov, Andrei; Armstrong, Scott A; Palacino, James; Buonamici, Silvia; Smith, Peter G; Bradley, Robert K; Abdel-Wahab, Omar. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins. Nature Medicine. 2016;22(6):672-8. PubMed - Comments: Used in: WB |
| Sciarrillo, Rocco; Wojtuszkiewicz, Anna; El Hassouni, Btissame; Funel, Niccola; Gandellini, Paolo; Lagerweij, Tonny; Buonamici, Silvia; Blijlevens, Maxime; Zeeuw van der Laan, Eveline A; Zaffaroni, Nadia; Deraco, Marcello; Kusamura, Shigeki; Würdinger, Tom; Peters, Godefridus J; Molthoff, Carla F M; Jansen, Gerrit; Kaspers, Gertjan J L; Cloos, Jacqueline; Giovannetti, Elisa. Splicing modulation as novel therapeutic strategy against diffuse malignant peritoneal mesothelioma. Ebiomedicine. 2019;39:215-225. PubMed - Comments: Used in: IHC |
| Cossa, Giacomo; Roeschert, Isabelle; Prinz, Florian; Baluapuri, Apoorva; Silveira Vidal, Raphael; Schülein-Völk, Christina; Chang, Yun-Chien; Ade, Carsten Patrick; Mastrobuoni, Guido; Girard, Cyrille; Wortmann, Lars; Walz, Susanne; Lührmann, Reinhard; Kempa, Stefan; Kuster, Bernhard; Wolf, Elmar; Mumberg, Dominik; Eilers, Martin. Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. Molecular Cell. 2020;77(6):1322-1339.e11. PubMed - Comments: Used in: WB |