Application: IEM, ELISA, IF
Clonality: Monoclonal
Host: Mouse
Reactivity: Sheep, Bovine, Equine, Porcine, Human
Pro-AKAP4 is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain (see picture).
AKAP4 (A-Kinase Anchor Protein 4) protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell.
The human AKAP4 gene is located on chromosome X and encodes a 854 amino acid polypeptide localized in the fibrous sheath of the sperm flagellum, AKAP4 plays a major role in sperm motility.
AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82.
The monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4. It does not react with the cleaved forms AKAP4.
Western blotting and immunocytochemistry techniques using the monoclonal anti-pro AKAP4 antibody clone 6F12 showed that the pro-AKAP4 is specifically expressed in human, equine, porcine, bovine murine and ovine spermatozoa.
Mouse monoclonal antibody clone 6F12 is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry (see applications).
Clonality: Monoclonal
Host: Mouse
Reactivity: Sheep, Bovine, Equine, Porcine, Human
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Mouse monoclonal antibody clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4) and is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry.Pro-AKAP4 is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain (see picture).
AKAP4 (A-Kinase Anchor Protein 4) protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell.
The human AKAP4 gene is located on chromosome X and encodes a 854 amino acid polypeptide localized in the fibrous sheath of the sperm flagellum, AKAP4 plays a major role in sperm motility.
AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82.
The monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4. It does not react with the cleaved forms AKAP4.
Western blotting and immunocytochemistry techniques using the monoclonal anti-pro AKAP4 antibody clone 6F12 showed that the pro-AKAP4 is specifically expressed in human, equine, porcine, bovine murine and ovine spermatozoa.
Mouse monoclonal antibody clone 6F12 is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry (see applications).
| Documents & Links for Anti-pro AKAP4 mAb (clone 6F12) | |
| Datasheet | Anti-pro AKAP4 mAb (clone 6F12) 100ug Datasheet |
| Datasheet | Anti-pro AKAP4 mAb (clone 6F12) 20ug Datasheet |
| Vendor Page | Anti-pro AKAP4 mAb (clone 6F12) at 4BioDx |
| Documents & Links for Anti-pro AKAP4 mAb (clone 6F12) | |
| Datasheet | Anti-pro AKAP4 mAb (clone 6F12) 100ug Datasheet |
| Datasheet | Anti-pro AKAP4 mAb (clone 6F12) 20ug Datasheet |
| Vendor Page | Anti-pro AKAP4 mAb (clone 6F12) |
| Citations for Anti-pro AKAP4 mAb (clone 6F12) – 8 Found |
| ProAKAP4 Semen Concentrations as a Valuable Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study. Vet Sci. 2022 May 6;9(5):224. doi: 10.3390/vetsci9050224. PubMed |
| Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility. J Clin Endocrinol Metab. 2022 Jun 16;107(7):1878-1887. doi: 10.1210/clinem/dgac218. PubMed |
| Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters. Reprod Fertil. 2021 Sep 23;2(4):268-279. doi: 10.1530/RAF-21-0055. eCollection 2021 Dec. PubMed |
| First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. 2021 Oct;102:133-135. doi: 10.1016/j.cryobiol.2021.07.009. Epub 2021 Jul 21. PubMed |
| Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 2020 Nov;160(5):695-707. doi: 10.1530/REP-20-0284. PubMed |
| Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 2020 Oct;18(5):409-414. doi: 10.1089/bio.2020.0019. Epub 2020 Aug 17. PubMed |
| Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: Promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. 2019 Jun;131:52-60. doi: 10.1016/j.theriogenology.2019.03.011. Epub 2019 Mar 21. PubMed |
| A Kinase Anchor Protein 4 Is Vulnerable to Oxidative Adduction in Male Germ Cells. Front Cell Dev Biol. 2019 Dec 20;7:319. doi: 10.3389/fcell.2019.00319. eCollection 2019. PubMed |