Immunofluorescence staining of A549 cells with CSB-RA618017A255phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Anti Phospho-SMAD2 (S255) mAb (Clone 2A12)

Catalog No:
CSB-RA618017A255phHU-50
$210.00
Application: IF, ELISA 
Clonality: Monoclonal 
Purification: Purified - Affinity 
Reactivity: Human 
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Documents & Links for Anti Phospho-SMAD2 (S255) mAb (Clone 2A12)
Datasheet Anti Phospho-SMAD2 (S255) mAb (Clone 2A12) Datasheet
Vendor Page Anti Phospho-SMAD2 (S255) mAb (Clone 2A12) at CUSABIO

Documents & Links for Anti Phospho-SMAD2 (S255) mAb (Clone 2A12)
Datasheet Anti Phospho-SMAD2 (S255) mAb (Clone 2A12) Datasheet
Vendor Page Anti Phospho-SMAD2 (S255) mAb (Clone 2A12)