Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
Monoclonal antibody targets NP95 for FCM, ICC, IHC, IPP, WB.
| Product Specifications | |
| Application | FC, ICC, IP, WB, IHC |
| Clonality | Monoclonal IgG2a ĸ |
| Host | Rat |
| Immunogen | Mouse thymic lymphoma |
| Formulation | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Citations for Anti-Np95 (Mouse) mAb (Clone: Th-10a) – 11 Found |
| Muto, M; Utsuyama, M; Horiguchi, T; Kubo, E; Sado, T; Hirokawa, K. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Proliferation. 1995;28(12):645-57. PubMed - Comments: Used in: IP |
| Muto, M; Utsuyama, M; Horiguchi, T; Kubo, E; Sado, T; Hirokawa, K. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Proliferation. 1995;28(12):645-57. PubMed - Comments: Used in: FCM |
| Muto, M; Utsuyama, M; Horiguchi, T; Kubo, E; Sado, T; Hirokawa, K. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Proliferation. 1995;28(12):645-57. PubMed - Comments: Used in: IHC |
| Fujimori, A; Matsuda, Y; Takemoto, Y; Hashimoto, Y; Kubo, E; Araki, R; Fukumura, R; Mita, K; Tatsumi, K; Muto, M. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mammalian Genome : Official Journal Of The International Mammalian Genome Society. 1998;9(12):1032-5. PubMed - Comments: Used in: WB |
| Fujimori, A; Matsuda, Y; Takemoto, Y; Hashimoto, Y; Kubo, E; Araki, R; Fukumura, R; Mita, K; Tatsumi, K; Muto, M. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mammalian Genome : Official Journal Of The International Mammalian Genome Society. 1998;9(12):1032-5. PubMed - Comments: Used in: IHC |
| Uemura, T; Kubo, E; Kanari, Y; Ikemura, T; Tatsumi, K; Muto, M. Temporal and spatial localization of novel nuclear protein NP95 in mitotic and meiotic cells. Cell Structure And Function. 2000;25(3):149-59. PubMed - Comments: Used in: ICC |
| Bonapace, Ian Marc; Latella, Lucia; Papait, Roberto; Nicassio, Francesco; Sacco, Alessandra; Muto, Masahiro; Crescenzi, Marco; Di Fiore, Pier Paolo. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. The Journal Of Cell Biology. 2002;157(6):909-14. PubMed - Comments: Used in: WB |
| Bonapace, Ian Marc; Latella, Lucia; Papait, Roberto; Nicassio, Francesco; Sacco, Alessandra; Muto, Masahiro; Crescenzi, Marco; Di Fiore, Pier Paolo. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. The Journal Of Cell Biology. 2002;157(6):909-14. PubMed - Comments: Used in: ICC |
| Sharif, Jafar; Muto, Masahiro; Takebayashi, Shin-ichiro; Suetake, Isao; Iwamatsu, Akihiro; Endo, Takaho A; Shinga, Jun; Mizutani-Koseki, Yoko; Toyoda, Tetsuro; Okamura, Kunihiro; Tajima, Shoji; Mitsuya, Kohzoh; Okano, Masaki; Koseki, Haruhiko. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 2007;450(7171):908-12. PubMed - Comments: Used in: ICC |
| Ohno, Rika; Nakayama, Megumi; Naruse, Chie; Okashita, Naoki; Takano, Osamu; Tachibana, Makoto; Asano, Masahide; Saitou, Mitinori; Seki, Yoshiyuki. A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells. Development (Cambridge, England). 2013;140(14):2892-903. PubMed - Comments: Used in: ICC |
| Maenohara, Shoji; Unoki, Motoko; Toh, Hidehiro; Ohishi, Hiroaki; Sharif, Jafar; Koseki, Haruhiko; Sasaki, Hiroyuki. Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos. Plos Genetics. 2017;13(10):e1007042. PubMed - Comments: Used in: IHC |