Background
Advanced Glycation End Product (AGE) is a general term for structures generated in the late stage of non-enzymatic glycation reactions (Millard reactions) between reducing sugars and proteins. AGE accumulation is known to be significantly elevated in age-related diseases such as diabetes and arteriosclerosis.
Nε-(carboxyethyl) lysine (CEL) is generated from protein modification by methylglyoxal (MG). MG is enzymatically derived from the Embden-Meyerhof and polyol pathways, through the degradation of glyceraldehyde-3-phosphate (G3P) (Phillips and Thornalley, 1993). Mclellan et al. (McLellan et al., 1994) demonstrated that plasma MG concentration in insulin-dependent diabetic patients was 7-times higher than in healthy individuals. CEL accumulation increases with age in human lens proteins.
Source: Professor Nagai Ryuji, Tokai University Faculty of Agriculture Department of Biosciences Food Bioregulation Research Laboratory
Product description
| Package Size | 50 µg |
|---|---|
| Form | Liquid (0.1M NaPB, pH7.0, 20 mg/ml BSA, 0.1% Sodium Azide (NaN3 ) added) |
| Concentration | 1 mg/mL |
| Purity | Affinity Purified |
| Host | Rabbit Polyclonal |
| Immunogen | Synthetic phosphopeptide corresponding to residues L(44)S(p)PFYLRPPSF(54)C of human alpha-B Crystallin |
| Specificity | Alpha-B crystallin p45S |
| Cross Reactivity | Bovine, Human, Mouse, Rat |
| Subclass | IgG |
| Storage | Store below -20°C. Avoid freeze-thaw cycles. |
References
Nagai R., Fujiwara Y., Mera K., Yamagata K., Sakashita N., Takeya M. Immunochemical detection of Nε-(carboxyethyl)lysine using a specific antibody. J. Immunol. Methods 332, 112-120 (2008). PMID: 18242632
| Product Specifications | |
| Application | ELISA, IF, WB, IHC |
| Clonality | Monoclonal (Clone No.: CEL-SP) |
| Host | Mouse |
| Documents & Links for Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) | |
| Datasheet | Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) Datasheet |
| Documents & Links for Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) | |
| Datasheet | Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) Datasheet |
| Citations for Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) – 1 Found |
| Koike, Shin; Ando, Chihiro; Usui, Yosuke; Kibune, Yosuke; Nishimoto, Shoichi; Suzuki, Toshihiro; Ogasawara, Yuki. Age-related alteration in the distribution of methylglyoxal and its metabolic enzymes in the mouse brain. Brain Research Bulletin. 2019;144( 30508605):164-170. PubMed |