Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.
Background
Advanced Glycation End Product (AGE) is a general term for structures generated in the late stage of non-enzymatic glycation reactions (Millard reactions) between reducing sugars and proteins. AGE accumulation is known to be significantly elevated in age-related diseases such as diabetes and arteriosclerosis.
Nε-(carboxyethyl) lysine (CEL) is generated from protein modification by methylglyoxal (MG). MG is enzymatically derived from the Embden-Meyerhof and polyol pathways, through the degradation of glyceraldehyde-3-phosphate (G3P) (Phillips and Thornalley, 1993). Mclellan et al. (McLellan et al., 1994) demonstrated that plasma MG concentration in insulin-dependent diabetic patients was 7-times higher than in healthy individuals. CEL accumulation increases with age in human lens proteins.
Source: Professor Nagai Ryuji, Tokai University Faculty of Agriculture Department of Biosciences Food Bioregulation Research Laboratory
Product description
| Package Size | 50 µg |
|---|---|
| Form | Liquid (0.1M NaPB, pH7.0, 20 mg/ml BSA, 0.1% Sodium Azide (NaN3 ) added) |
| Concentration | 1 mg/mL |
| Purity | Affinity Purified |
| Host | Rabbit Polyclonal |
| Immunogen | Synthetic phosphopeptide corresponding to residues L(44)S(p)PFYLRPPSF(54)C of human alpha-B Crystallin |
| Specificity | Alpha-B crystallin p45S |
| Cross Reactivity | Bovine, Human, Mouse, Rat |
| Subclass | IgG |
| Storage | Store below -20°C. Avoid freeze-thaw cycles. |
References
Nagai R., Fujiwara Y., Mera K., Yamagata K., Sakashita N., Takeya M. Immunochemical detection of Nε-(carboxyethyl)lysine using a specific antibody. J. Immunol. Methods 332, 112-120 (2008). PMID: 18242632
| Product Specifications | |
| Application | ELISA, IF, WB, IHC |
| Clonality | Monoclonal (Clone No.: CEL-SP) |
| Host | Mouse |
| Documents & Links for Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) | |
| Datasheet | Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) Datasheet |
| Documents & Links for Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) | |
| Datasheet | Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) Datasheet |
| Citations for Anti N(Epsilon)-(Carboxyethyl) Lysine (CEL) mAb (Clone CEL-SP) – 1 Found |
| Koike, Shin; Ando, Chihiro; Usui, Yosuke; Kibune, Yosuke; Nishimoto, Shoichi; Suzuki, Toshihiro; Ogasawara, Yuki. Age-related alteration in the distribution of methylglyoxal and its metabolic enzymes in the mouse brain. Brain Research Bulletin. 2019;144( 30508605):164-170. PubMed |