Anti-LC3 (Human) mAb (Clone: 4E12)

MBL

Catalog No: MBL-M152-3

Browse all MBL products from our MBL products homepage.

This LC3 antibody is validated for multiple applications (ICC, IH, IP, FCM, Immuno-EM) and has numerous citations. This is a monoclonal antibody targeting LC3 for FCM, ICC, IP. More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.

Product Specifications

• Application: FC, ICC, IP, IHC, Immuno-EM
• Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Monoclonal IgG1 ĸ
• Host: Mouse
• Immunogen: Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)
• Formulation: 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Citations for Anti-LC3 (Human) mAb (Clone: 4E12) – 57 Found

Thayanidhi, Nandhakumar; Helm, Jared R; Nycz, Deborah C; Bentley, Marvin; Liang, Yingjian; Hay, Jesse C. Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREs. Molecular Biology Of The Cell. 2010;21(11):1850-63. 
- Comments: Used in: ICC

Eng, Kai Er; Panas, Marc D; Karlsson Hedestam, Gunilla B; McInerney, Gerald M. A novel quantitative flow cytometry-based assay for autophagy. Autophagy. 2010;6(5):634-41. 
- Comments: Used in: FCM

Saiki, Shinji; Sasazawa, Yukiko; Imamichi, Yoko; Kawajiri, Sumihiro; Fujimaki, Takahiro; Tanida, Isei; Kobayashi, Hiroki; Sato, Fumiaki; Sato, Shigeto; Ishikawa, Ken-Ichi; Imoto, Masaya; Hattori, Nobutaka. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy. 2011;7(2):176-87. 
- Comments: Used in: WB

Ho, Hsiang; Kapadia, Rubina; Al-Tahan, Sejad; Ahmad, Safoora; Ganesan, Anand K. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. The Journal Of Biological Chemistry. 2011;286(14):12509-23. 
- Comments: Used in: WB

Kovacs, J R; Li, C; Yang, Q; Li, G; Garcia, I G; Ju, S; Roodman, D G; Windle, J J; Zhang, X; Lu, B. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death And Differentiation. 2012;19(1):144-52. 
- Comments: Used in: ICC

Cai, T; Hirai, H; Zhang, G; Zhang, M; Takahashi, N; Kasai, H; Satin, L S; Leapman, R D; Notkins, A L. Deletion of Ia-2 and/or Ia-2β in mice decreases insulin secretion by reducing the number of dense core vesicles. Diabetologia. 2011;54(9):2347-57. 
- Comments: Used in: ICC

Traver, Maria K; Henry, Stanley C; Cantillana, Viviana; Oliver, Tim; Hunn, Julia P; Howard, Jonathan C; Beer, Sandra; Pfeffer, Klaus; Coers, Jörn; Taylor, Gregory A. Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. The Journal Of Biological Chemistry. 2011;286(35):30471-30480. 
- Comments: Used in: ICC

Moreau, Kevin; Ravikumar, Brinda; Renna, Maurizio; Puri, Claudia; Rubinsztein, David C. Autophagosome precursor maturation requires homotypic fusion. Cell. 2011;146(2):303-17. 
- Comments: Used in: ICC

Gillis, Judith M; Benckhuijsen, Willemien; van Veen, Henk; Sanz, Alicia Sanz; Drijfhout, Jan W; Reits, Eric A. Aminopeptidase-resistant peptides are targeted to lysosomes and subsequently degraded. Traffic (Copenhagen, Denmark). 2011;12(12):1897-910. 
- Comments: Used in: ICC

Frankel, Lisa B; Wen, Jiayu; Lees, Michael; Høyer-Hansen, Maria; Farkas, Thomas; Krogh, Anders; Jäättelä, Marja; Lund, Anders H. microRNA-101 is a potent inhibitor of autophagy. The Embo Journal. 2011;30(22):4628-41. 
- Comments: Used in: WB

Chalazonitis, Alcmène; Tang, Amy A; Shang, Yulei; Pham, Tuan D; Hsieh, Ivy; Setlik, Wanda; Gershon, Michael D; Huang, Eric J. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience. 2011;31(39):13746-57. 
- Comments: Used in: IHC

Seto, Shintaro; Tsujimura, Kunio; Koide, Yukio. Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. Cellular Microbiology. 2012;14(5):710-27. 
- Comments: Used in: ICC

Starr, Tregei; Child, Robert; Wehrly, Tara D; Hansen, Bryan; Hwang, Seungmin; López-Otin, Carlos; Virgin, Herbert W; Celli, Jean. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host & Microbe. 2012;11(1):33-45. 
- Comments: Used in: WB

Starr, Tregei; Child, Robert; Wehrly, Tara D; Hansen, Bryan; Hwang, Seungmin; López-Otin, Carlos; Virgin, Herbert W; Celli, Jean. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host & Microbe. 2012;11(1):33-45. 
- Comments: Used in: ICC

Shi, Chong-Shan; Shenderov, Kevin; Huang, Ning-Na; Kabat, Juraj; Abu-Asab, Mones; Fitzgerald, Katherine A; Sher, Alan; Kehrl, John H. Activation of autophagy by inflammatory signals limits IL-1β production by targeting ubiquitinated inflammasomes for destruction. Nature Immunology. 2012;13(3):255-63. 
- Comments: Used in: WB

Sims, Joshua J; Scavone, Francesco; Cooper, Eric M; Kane, Lesley A; Youle, Richard J; Boeke, Jef D; Cohen, Robert E. Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling. Nature Methods. 2012;9(3):303-9. 
- Comments: Used in: ICC

McKnight, Nicole C; Jefferies, Harold B J; Alemu, Endalkachew A; Saunders, Rebecca E; Howell, Michael; Johansen, Terje; Tooze, Sharon A. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. The Embo Journal. 2012;31(8):1931-46. 
- Comments: Used in: ICC

Morris, Chantey R; Stanton, Marissa J; Manthey, Karoline C; Oh, Keon Bong; Wagner, Kay-Uwe. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. Plos One. 7(3):e34308. 
- Comments: Used in: WB

Morris, Chantey R; Stanton, Marissa J; Manthey, Karoline C; Oh, Keon Bong; Wagner, Kay-Uwe. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. Plos One. 7(3):e34308. 
- Comments: Used in: ICC

Joubert, Pierre-Emmanuel; Werneke, Scott W; de la Calle, Claire; Guivel-Benhassine, Florence; Giodini, Alessandra; Peduto, Lucie; Levine, Beth; Schwartz, Olivier; Lenschow, Deborah J; Albert, Matthew L. Chikungunya virus-induced autophagy delays caspase-dependent cell death. The Journal Of Experimental Medicine. 2012;209(5):1029-47. 
- Comments: Used in: WB

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 
- Comments: Used in: WB

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 

Matsuzawa, Yu; Oshima, Shigeru; Takahara, Masahiro; Maeyashiki, Chiaki; Nemoto, Yasuhiro; Kobayashi, Masanori; Nibe, Yoichi; Nozaki, Kengo; Nagaishi, Takashi; Okamoto, Ryuichi; Tsuchiya, Kiichiro; Nakamura, Tetsuya; Ma, Averil; Watanabe, Mamoru. TNFAIP3 promotes survival of CD4 T cells by restricting MTOR and promoting autophagy. Autophagy. 11(7):1052-62. 
- Comments: Used in: ICC

Silva, Bruno Jorge de Andrade; Barbosa, Mayara Garcia de Mattos; Andrade, Priscila Ribeiro; Ferreira, Helen; Nery, José Augusto da Costa; Côrte-Real, Suzana; da Silva, Gilberto Marcelo Sperandio; Rosa, Patricia Sammarco; Fabri, Mario; Sarno, Euzenir Nunes; Pinheiro, Roberta Olmo. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. Plos Pathogens. 2017;13(1):e1006103. 
- Comments: Used in: WB

Silva, Bruno Jorge de Andrade; Barbosa, Mayara Garcia de Mattos; Andrade, Priscila Ribeiro; Ferreira, Helen; Nery, José Augusto da Costa; Côrte-Real, Suzana; da Silva, Gilberto Marcelo Sperandio; Rosa, Patricia Sammarco; Fabri, Mario; Sarno, Euzenir Nunes; Pinheiro, Roberta Olmo. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. Plos Pathogens. 2017;13(1):e1006103. 
- Comments: Used in: ICC

Silva, Bruno Jorge de Andrade; Barbosa, Mayara Garcia de Mattos; Andrade, Priscila Ribeiro; Ferreira, Helen; Nery, José Augusto da Costa; Côrte-Real, Suzana; da Silva, Gilberto Marcelo Sperandio; Rosa, Patricia Sammarco; Fabri, Mario; Sarno, Euzenir Nunes; Pinheiro, Roberta Olmo. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. Plos Pathogens. 2017;13(1):e1006103. 
- Comments: Used in: IHC

Abe, Shoko; Nagai, Tomoaki; Masukawa, Moe; Okumoto, Kanji; Homma, Yuta; Fujiki, Yukio; Mizuno, Kensaku. Localization of Protein Kinase NDR2 to Peroxisomes and Its Role in Ciliogenesis. The Journal Of Biological Chemistry. 2017;292(10):4089-4098. 
- Comments: Used in: ICC

Nakano, Satoshi; Oki, Mitsuaki; Kusaka, Hirofumi. The role of p62/SQSTM1 in sporadic inclusion body myositis. Neuromuscular Disorders : Nmd. 2017;27(4):363-369. 
- Comments: Used in: IHC

Ye, Mao; Qiu, Hong; Cao, Yingkang; Zhang, Min; Mi, Yan; Yu, Jing; Wang, Changhua. Curcumin Improves Palmitate-Induced Insulin Resistance in Human Umbilical Vein Endothelial Cells by Maintaining Proteostasis in Endoplasmic Reticulum. Frontiers In Pharmacology. 8:148. 
- Comments: Used in: WB

Hasegawa, Haruki; Hsu, Ann; Tinberg, Christine E; Siegler, Karen E; Nazarian, Aaron A; Tsai, Mei-Mei. Single amino acid substitution in LC-CDR1 induces Russell body phenotype that attenuates cellular protein synthesis through eIF2α phosphorylation and thereby downregulates IgG secretion despite operational secretory pathway traffic. Mabs. 2017;9(5):854-873. 
- Comments: Used in: ICC

Arasaki, Kohei; Mikami, Yumi; Shames, Stephanie R; Inoue, Hiroki; Wakana, Yuichi; Tagaya, Mitsuo. Legionella effector Lpg1137 shuts down ER-mitochondria communication through cleavage of syntaxin 17. Nature Communications. 2017;8:15406. 
- Comments: Used in: ICC

Wacker, Rahel; Eickel, Nina; Schmuckli-Maurer, Jacqueline; Annoura, Takeshi; Niklaus, Livia; Khan, Shahid M; Guan, Jun-Lin; Heussler, Volker T. LC3-association with the parasitophorous vacuole membrane of Plasmodium berghei liver stages follows a noncanonical autophagy pathway. Cellular Microbiology. 2017;19(10) 
- Comments: Used in: ICC

Kimura, Tetsuya; Suzuki, Mamiko; Akagi, Takumi. Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus. Acta Neuropathologica Communications. 2017;5(1):67. 
- Comments: Used in: WB

Kimura, Tetsuya; Suzuki, Mamiko; Akagi, Takumi. Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus. Acta Neuropathologica Communications. 2017;5(1):67. 
- Comments: Used in: IHC

Zhao, Qiong; Hu, Zhan-Ying; Zhang, Jing-Pu; Jiang, Jian-Dong; Ma, Yuan-Yuan; Li, Jian-Rui; Peng, Zong-Gen; Chen, Jin-Hua. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Scientific Reports. 2017;7(1):11250. 
- Comments: Used in: ICC

Sato, Yusuke; Ohtsubo, Hideaki; Nihei, Naohiro; Kaneko, Takane; Sato, Yoriko; Adachi, Shin-Ichi; Kondo, Shinji; Nakamura, Mako; Mizunoya, Wataru; Iida, Hiroshi; Tatsumi, Ryuichi; Rada, Cristina; Yoshizawa, Fumiaki. Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle. Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology. 2018;32(3):1428-1439. 
- Comments: Used in: IHC

Newton, Timothy; Allison, Rachel; Edgar, James R; Lumb, Jennifer H; Rodger, Catherine E; Manna, Paul T; Rizo, Tania; Kohl, Zacharias; Nygren, Anders O H; Arning, Larissa; Schüle, Rebecca; Depienne, Christel; Goldberg, Lisa; Frahm, Christiane; Stevanin, Giovanni; Durr, Alexandra; Schöls, Ludger; Winner, Beate; Beetz, Christian; Reid, Evan. Mechanistic basis of an epistatic interaction reducing age at onset in hereditary spastic paraplegia. Brain : A Journal Of Neurology. 2018;141(5):1286-1299. 
- Comments: Used in: ICC

Takahashi, Akiko; Loo, Tze Mun; Okada, Ryo; Kamachi, Fumitaka; Watanabe, Yoshihiro; Wakita, Masahiro; Watanabe, Sugiko; Kawamoto, Shimpei; Miyata, Kenichi; Barber, Glen N; Ohtani, Naoko; Hara, Eiji. Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells. Nature Communications. 2018;9(1):1249. 
- Comments: Used in: WB

Konno, Hiroyasu; Chinn, Ivan K; Hong, Diana; Orange, Jordan S; Lupski, James R; Mendoza, Alejandra; Pedroza, Luis A; Barber, Glen N. Pro-inflammation Associated with a Gain-of-Function Mutation (R284S) in the Innate Immune Sensor STING. Cell Reports. 2018;23(4):1112-1123. 
- Comments: Used in: ICC

Perot, Brieuc P; Boussier, Jeremy; Yatim, Nader; Rossman, Jeremy S; Ingersoll, Molly A; Albert, Matthew L. Autophagy diminishes the early interferon-β response to influenza A virus resulting in differential expression of interferon-stimulated genes. Cell Death & Disease. 2018;9(5):539. 

Wise, John P Jr; Price, Charles G; Amaro, Joseph A; Cannon, Jason R. Autophagy Disruptions Associated With Altered Optineurin Expression in Extranigral Regions in a Rotenone Model of Parkinson's Disease. Frontiers In Neuroscience. 12:289. 
- Comments: Used in: ICC

Kanda, Ranka; Hiraike, Haruko; Wada-Hiraike, Osamu; Ichinose, Takayuki; Nagasaka, Kazunori; Sasajima, Yuko; Ryo, Eiji; Fujii, Tomoyuki; Osuga, Yutaka; Ayabe, Takuya. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. Bmc Cancer. 2018;18(1):657. 
- Comments: Used in: WB

Kanda, Ranka; Hiraike, Haruko; Wada-Hiraike, Osamu; Ichinose, Takayuki; Nagasaka, Kazunori; Sasajima, Yuko; Ryo, Eiji; Fujii, Tomoyuki; Osuga, Yutaka; Ayabe, Takuya. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. Bmc Cancer. 2018;18(1):657. 
- Comments: Used in: ICC

Mori, Hiroyuki; Fukuhara, Takasuke; Ono, Chikako; Tamura, Tomokazu; Sato, Asuka; Fauzyah, Yuzy; Wada, Masami; Okamoto, Toru; Noda, Takeshi; Yoshimori, Tamotsu; Matsuura, Yoshiharu. Induction of selective autophagy in cells replicating hepatitis C virus genome. The Journal Of General Virology. 2018;99(12):1643-1657. 
- Comments: Used in: ICC

Kajiume, Teruyuki; Kobayashi, Masao. Human granulocytes undergo cell death via autophagy. Cell Death Discovery. 4:111. 
- Comments: Used in: ICC

Zemirli, Naïma; Boukhalfa, Asma; Dupont, Nicolas; Botti, Joëlle; Codogno, Patrice; Morel, Etienne. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 2019;3(3):100-109. 
- Comments: Used in: IF

Hou, Bolin; Wang, Gang; Gao, Quan; Wei, Yanjie; Zhang, Caining; Wang, Yange; Huo, Yuqing; Yang, Huaiyi; Jiang, Xuejun; Xi, Zhijun. SQSTM1/p62 loss reverses the inhibitory effect of sunitinib on autophagy independent of AMPK signaling. Scientific Reports. 2019;9(1):11087. 
- Comments: Used in: IP

Bordi, Matteo; Darji, Sandipkumar; Sato, Yutaka; Mellén, Marian; Berg, Martin J; Kumar, Asok; Jiang, Ying; Nixon, Ralph A. mTOR hyperactivation in Down Syndrome underlies deficits in autophagy induction, autophagosome formation, and mitophagy. Cell Death & Disease. 2019;10(8):563. 
- Comments: Used in: ICC

Lasič, Eva; Lisjak, Marjeta; Horvat, Anemari; Božić, Mićo; Šakanović, Aleksandra; Anderluh, Gregor; Verkhratsky, Alexei; Vardjan, Nina; Jorgačevski, Jernej; Stenovec, Matjaž; Zorec, Robert. Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action. Scientific Reports. 2019;9(1):10957. 
- Comments: Used in: ICC

Vrahnas, Christina; Blank, Martha; Dite, Toby A; Tatarczuch, Liliana; Ansari, Niloufar; Crimeen-Irwin, Blessing; Nguyen, Huynh; Forwood, Mark R; Hu, Yifang; Ikegame, Mika; Bambery, Keith R; Petibois, Cyril; Mackie, Eleanor J; Tobin, Mark J; Smyth, Gordon K; Oakhill, Jonathan S; Martin, T John; Sims, Natalie A. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nature Communications. 2019;10(1):5073. 
- Comments: Used in: ICC

Vrahnas, Christina; Blank, Martha; Dite, Toby A; Tatarczuch, Liliana; Ansari, Niloufar; Crimeen-Irwin, Blessing; Nguyen, Huynh; Forwood, Mark R; Hu, Yifang; Ikegame, Mika; Bambery, Keith R; Petibois, Cyril; Mackie, Eleanor J; Tobin, Mark J; Smyth, Gordon K; Oakhill, Jonathan S; Martin, T John; Sims, Natalie A. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nature Communications. 2019;10(1):5073. 
- Comments: Used in: WB

Ye, Xiaoxia; Zhu, Mingming; Che, Xiaohang; Wang, Huiyang; Liang, Xing-Jie; Wu, Chunfu; Xue, Xue; Yang, Jingyu. Lipopolysaccharide induces neuroinflammation in microglia by activating the MTOR pathway and downregulating Vps34 to inhibit autophagosome formation. Journal Of Neuroinflammation. 2020;17(1):18. 
- Comments: Used in: ICC

Boukhalfa, Asma; Nascimbeni, Anna Chiara; Ramel, Damien; Dupont, Nicolas; Hirsch, Emilio; Gayral, Stephanie; Laffargue, Muriel; Codogno, Patrice; Morel, Etienne. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nature Communications. 2020;11(1):294. 
- Comments: Used in: ICC

Du, Ting-Ting; Zhu, Guanyu; Chen, Yingchuan; Shi, Lin; Liu, Defeng; Liu, Yuye; Zhang, Xin; Zhang, Jianguo. Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys. Aging. 2020;12(7):6324-6339. 
- Comments: Used in: WB

Rahaman, Md Shiblur; Banik, Subrata; Akter, Mahmuda; Rahman, Md Mostafizur; Sikder, Md Tajuddin; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki. Curcumin alleviates arsenic-induced toxicity in PC12 cells via modulating autophagy/apoptosis. Ecotoxicology And Environmental Safety. 2020;200:110756. 
- Comments: Used in: WB

Rahaman, Md Shiblur; Yamasaki, Shojiro; Binte Hossain, Kaniz Fatima; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki. Effects of curcumin, D-pinitol alone or in combination in cytotoxicity induced by arsenic in PC12 cells. Food And Chemical Toxicology : An International Journal Published For The British Industrial Biological Research Association. 2020;144:111577. 
- Comments: Used in: WB