Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbial pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
Monoclonal antibody for targeting IL-18 for ELISA, IC, IPP, NT.
| Product Specifications | |
| Application | IP, ELISA, Neutralization |
| Reactivity | Human |
| Clonality | Monoclonal IgG1 |
| Host | Mouse |
| Immunogen | Recombinant human IL-18 |
| Gene ID - Human | |
| Formulation | 100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Citations for Anti-IL-18 (Clone 125-2H) (Human) mAb (Clone: 125-2H) – 16 Found |
| Shida, K; Shiratori, I; Matsumoto, M; Fukumori, Y; Matsuhisa, A; Kikkawa, S; Tsuji, S; Okamura, H; Toyoshima, K; Seya, T. An alternative form of IL-18 in human blood plasma: complex formation with IgM defined by monoclonal antibodies. Journal Of Immunology (Baltimore, Md. : 1950). 2001;166(11):6671-9. PubMed - Comments: Used in: ELISA |
| Sugawara, S; Uehara, A; Nochi, T; Yamaguchi, T; Ueda, H; Sugiyama, A; Hanzawa, K; Kumagai, K; Okamura, H; Takada, H. Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion by human oral epithelial cells. Journal Of Immunology (Baltimore, Md. : 1950). 2001;167(11):6568-75. PubMed - Comments: Used in: NT |
| Skurk, Thomas; Kolb, Hubert; Müller-Scholze, Sylvia; Röhrig, Karin; Hauner, Hans; Herder, Christian. The proatherogenic cytokine interleukin-18 is secreted by human adipocytes. European Journal Of Endocrinology. 2005;152(6):863-8. PubMed - Comments: Used in: ICC |
| Tu, Zhengkun; Bozorgzadeh, Adel; Pierce, Robert H; Kurtis, Jonathan; Crispe, I Nicholas; Orloff, Mark S. TLR-dependent cross talk between human Kupffer cells and NK cells. The Journal Of Experimental Medicine. 2008;205(1):233-44. PubMed - Comments: Used in: NT |
| Nussbaumer, Oliver; Gruenbacher, Georg; Gander, Hubert; Thurnher, Martin. DC-like cell-dependent activation of human natural killer cells by the bisphosphonate zoledronic acid is regulated by γδ T lymphocytes. Blood. 2011;118(10):2743-51. PubMed - Comments: Used in: NT |
| Okano, Mitsuhiro; Fujiwara, Tazuko; Makihara, Seiichiro; Fujiwara, Rumi; Higaki, Takaya; Kariya, Shin; Noda, Yohei; Haruna, Takenori; Nishizaki, Kazunori. Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis. International Archives Of Allergy And Immunology. 160(3):275-86. PubMed - Comments: Used in: ELISA |
| Thinwa, Josephine; Segovia, Jesus A; Bose, Santanu; Dube, Peter H. Integrin-mediated first signal for inflammasome activation in intestinal epithelial cells. Journal Of Immunology (Baltimore, Md. : 1950). 2014;193(3):1373-82. PubMed - Comments: Used in: ELISA |
| Guerville, Florent; Daburon, Sophie; Marlin, Romain; Lartigue, Lydia; Loizon, Severine; Pitard, Vincent; Couzi, Lionel; Moreau, Jean-François; Déchanet-Merville, Julie; Faustin, Benjamin. TCR-dependent sensitization of human γδ T cells to non-myeloid IL-18 in cytomegalovirus and tumor stress surveillance. Oncoimmunology. 2015;4(5):e1003011. PubMed - Comments: Used in: Function |
| van Wilgenburg, Bonnie; Scherwitzl, Iris; Hutchinson, Edward C; Leng, Tianqi; Kurioka, Ayako; Kulicke, Corinna; de Lara, Catherine; Cole, Suzanne; Vasanawathana, Sirijitt; Limpitikul, Wannee; Malasit, Prida; Young, Duncan; Denney, Laura; Moore, Michael D; Fabris, Paolo; Giordani, Maria Teresa; Oo, Ye Htun; Laidlaw, Stephen M; Dustin, Lynn B; Ho, Ling-Pei; Thompson, Fiona M; Ramamurthy, Narayan; Mongkolsapaya, Juthathip; Willberg, Christian B; Screaton, Gavin R; Klenerman, Paul. MAIT cells are activated during human viral infections. Nature Communications. 2016;7:11653. PubMed - Comments: Used in: Function |
| Furman, David; Chang, Junlei; Lartigue, Lydia; Bolen, Christopher R; Haddad, François; Gaudilliere, Brice; Ganio, Edward A; Fragiadakis, Gabriela K; Spitzer, Matthew H; Douchet, Isabelle; Daburon, Sophie; Moreau, Jean-François; Nolan, Garry P; Blanco, Patrick; Déchanet-Merville, Julie; Dekker, Cornelia L; Jojic, Vladimir; Kuo, Calvin J; Davis, Mark M; Faustin, Benjamin. Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states. Nature Medicine. 2017;23(2):174-184. PubMed - Comments: Used in: ELISA |
| Lagrange, Brice; Benaoudia, Sacha; Wallet, Pierre; Magnotti, Flora; Provost, Angelina; Michal, Fanny; Martin, Amandine; Di Lorenzo, Flaviana; Py, Bénédicte F; Molinaro, Antonio; Henry, Thomas. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11. Nature Communications. 2018;9(1):242. PubMed - Comments: Used in: ELISA |
| Allam, Ossama; Samarani, Suzanne; Mehraj, Vikram; Jenabian, Mohammad-Ali; Tremblay, Cecile; Routy, Jean-Pierre; Amre, Devendra; Ahmad, Ali. HIV induces production of IL-18 from intestinal epithelial cells that increases intestinal permeability and microbial translocation. Plos One. 13(3):e0194185. PubMed - Comments: Used in: NT |
| Bossù, Paola; Salani, Francesca; Ciaramella, Antonio; Sacchinelli, Eleonora; Mosca, Alessandra; Banaj, Nerisa; Assogna, Francesca; Orfei, Maria Donata; Caltagirone, Carlo; Gianni, Walter; Spalletta, Gianfranco. Anti-inflammatory Effects of Homotaurine in Patients With Amnestic Mild Cognitive Impairment. Frontiers In Aging Neuroscience. 10:285. PubMed - Comments: Used in: ELISA |
| Pal, Arumay; Neo, Kurt; Rajamani, Lakshminarayanan; Ferrer, Fernando Jose; Lane, David P; Verma, Chandra S; Mortellaro, Alessandra. Inhibition of NLRP3 inflammasome activation by cell-permeable stapled peptides. Scientific Reports. 2019;9(1):4913. PubMed - Comments: Used in: ELISA |
| Santegoets, Saskia J; Duurland, Chantal L; Jordanova, Ekaterina J; van Ham, Vanessa J; Ehsan, Ilina; Loof, Nikki M; Narang, Vipin; Dutertre, Charles A; Ginhoux, Florent; van Egmond, Sylvia L; J P Welters, Marij; van der Burg, Sjoerd H. CD163(+) cytokine-producing cDC2 stimulate intratumoral type 1 T cell responses in HPV16-induced oropharyngeal cancer. Journal For Immunotherapy Of Cancer. 2020;8(2) PubMed - Comments: Used in: NT |
| Kameda, Masami; Otsuka, Mitsuo; Chiba, Hirofumi; Kuronuma, Koji; Hasegawa, Takehiro; Takahashi, Hiroki; Takahashi, Hiroki. CXCL9, CXCL10, and CXCL11; biomarkers of pulmonary inflammation associated with autoimmunity in patients with collagen vascular diseases-associated interstitial lung disease and interstitial pneumonia with autoimmune features. Plos One. 15(11):e0241719. PubMed - Comments: Used in: ELISA |