Anti Histone H3.1 (HIST1H3A) T11ph mAb (Clone 6G12C5)

Cosmo Bio LTD

Catalog No: CAC-CEC-002

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Background
Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Phosphorylation of histone H3 Threonine 11 (H3-T11ph) occurs throughout the cell cycle and is Chk1 dependent. It has been reported that DNA damage rapidly reduces H3-T11 phosphorylation.

Application
・ Western blotting: 1/500
・ Immunocytochemistry: 1/500

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

The composition of Histone H3 peptides and the reactivity of Histone H3 T11ph antibody, 6G12C5.

H3                      1  ARTKQTARK    S     T   GGKAPRKQC   19
H3 K9ac               1  ARTKQTARKac S     T   GGKAPRKQC   19
H3 S10ph             1  ARTKQTARK    Sph T   GGKAPRKQC   19
H3 T11ph             1  ARTKQTARK    S     TphGGKAPRKQC   19
H3 K9acT11ph       1  ARTKQTARKac S     TphGGKAPRKQC   19
H3 S10phT11ph     1  ARTKQTARK    Sph TphGGKAPRKQC   19
H3.1 T32ph           21   ATKAARKSAPA     TphGGVKKPH       39
H3.3 T32ph           21   ATKAARKSAPS     TphGGVKKPH       39 

References
Shimada M, et al. (2008) Cell. 132(2):221-32. PMID: 18243098.

Product Specifications

• Application: ICC, WB, ELISA
• Reactivity: Hamster, Monkey, Mouse, Rat, Human
• Clonality: Monoclonal (Clone No.: 6G12C5)
• Host: Rat