Anti Histone H3.1 (HIST1H3A) S10ph mAb (Clone 6G8B7)

Cosmo Bio

Catalog No: CAC-CEC-001

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Product Description

Background
Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Phosphorylation of histone H3 is unique in the sense that it associates on one hand with open chromatin during gene activation and marks on the other hand highly condensed chromatin during mitosis.

Application
・ Western blotting: 1/2500
・ Immunocytochemistry: 1/5000

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

The composition of Histone H3 peptides and the reactivity of Histone H3 S10ph antibody, 6G8B7.

H3 peptide               1   ARTKQTARKS   TGGKAPRKQ      19
H3 S10ph peptide　　　1   ARTKQTARKSphTGGKAPRKQ      19
H3 S28ph peptide     21      ATKAARKSphAPATGGVKKPH   39

References
1) Strahl and Allis, (2000) Nature. 403(6765):41-5. PMID: 10638745

Product Specifications

• Application: ICC, WB, ELISA
• Reactivity: Monkey, Mouse, Rat, Human, Hamster
• Clonality: Monoclonal (Clone No.: 6G8B7)
• Host: Rat