Polyclonal Antibody conjugated to HRP of 50 µl targeting GST-tag for WB.
HRP-DirecT is a series of HRP conjugated primary antibodies that doesn't need secondary antibodies. That brings the following advantages:
① Total incubation time is cut in half.
The reaction with the secondary antibody becomes unnecessary. In the shaking method, reaction time is only 30 minutes.
② Clear result
No more heavy and light chain bands in Immunoprecipitation. The HRP conjugated primary antibody will not detect your precipitating antibody. Usually, the secondary antibody amplifies the signal of an unconjugated primary antibody. Therefore, it is thought to be difficult to obtain a strong signal with a directly conjugated antibody. To overcome this hurdle, MBLI has improved the HRP conjugation method. HRP-DirecT Series yields a strong signal with minimum background.
| Product Specifications | |
| Application | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Recombinant GST |
| Formulation | PBS/Preservative/Stabilizer |
| Citations for Anti-GST-tag pAb-HRP-DirecT – 8 Found |
| Tomita, Takeshi; Ieguchi, Katsuaki; Sawamura, Tatsuya; Maru, Yoshiro. Human serum amyloid A3 (SAA3) protein, expressed as a fusion protein with SAA2, binds the oxidized low density lipoprotein receptor. Plos One. 10(3):e0118835. PubMed - Comments: Used in: WB |
| Ueyama, Takehiko; Ninoyu, Yuzuru; Nishio, Shin-Ya; Miyoshi, Takushi; Torii, Hiroko; Nishimura, Koji; Sugahara, Kazuma; Sakata, Hideaki; Thumkeo, Dean; Sakaguchi, Hirofumi; Watanabe, Naoki; Usami, Shin-Ichi; Saito, Naoaki; Kitajiri, Shin-Ichiro. Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss. Embo Molecular Medicine. 2016;8(11):1310-1324. PubMed - Comments: Used in: WB |
| Hara, Masatoshi; Lourido, Sebastian; Petrova, Boryana; Lou, Hua Jane; Von Stetina, Jessica R; Kashevsky, Helena; Turk, Benjamin E; Orr-Weaver, Terry L. Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition. Elife. 2018;7 PubMed - Comments: Used in: WB |
| Senga, Shogo; Kawaguchi, Koichiro; Kobayashi, Narumi; Ando, Akira; Fujii, Hiroshi. A novel fatty acid-binding protein 5-estrogen-related receptor α signaling pathway promotes cell growth and energy metabolism in prostate cancer cells. Oncotarget. 2018;9(60):31753-31770. PubMed - Comments: Used in: WB |
| Mizoi, Junya; Kanazawa, Natsumi; Kidokoro, Satoshi; Takahashi, Fuminori; Qin, Feng; Morimoto, Kyoko; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko. Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance of Arabidopsis thaliana. The Journal Of Biological Chemistry. 2019;294(3):902-917. PubMed - Comments: Used in: WB |
| Xie, Yurong; Liu, Yang; Ma, Mengdi; Zhou, Qin; Zhao, Yongping; Zhao, Binbin; Wang, Baobao; Wei, Hongbin; Wang, Haiyang. Arabidopsis FHY3 and FAR1 integrate light and strigolactone signaling to regulate branching. Nature Communications. 2020;11(1):1955. PubMed - Comments: Used in: WB |
| Kido, Kohki; Yamanaka, Satoshi; Nakano, Shogo; Motani, Kou; Shinohara, Souta; Nozawa, Akira; Kosako, Hidetaka; Ito, Sohei; Sawasaki, Tatsuya. AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions. Elife. 2020;9 PubMed - Comments: Used in: WB |
| Yamashita, Yumi; Yamashita, Toshiyuki. Novel phospho-specific monoclonal antibodies reveal differential regulation of tyrosine phosphorylation within the immunoreceptor tyrosine-based activation motif of the Fc receptor γ subunit leading to fine tuning of Syk activation. Biochemical And Biophysical Research Communications. 2021;547:89-95. PubMed - Comments: Used in: WB |