Anti-GFP (Green Fluorescent Protein) mAb-Agarose (Clone: RQ2)

Catalog No:
MBL-D153-8

Availability: 12PM PT on Dec. 3, 2024

Monoclonal antibody of 10 µl targeting GFP for ICC, IPP conjugated to agarose.



Product Specifications
Application IP
Clonality Monoclonal IgG2a
Host Rat
Immunogen GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP
Formulation 100 mg of anti-GFP monoclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL.

Citations for Anti-GFP (Green Fluorescent Protein) mAb-Agarose (Clone: RQ2) – 43 Found
Darzacq, Xavier; Kittur, Nupur; Roy, Sujayita; Shav-Tal, Yaron; Singer, Robert H; Meier, U Thomas. Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells. The Journal Of Cell Biology. 2006;173(2):207-18.  PubMed
Comments: Used in: ChIP
Hayakawa, Toshihiko; Kudo, Toru; Ito, Takashi; Takahashi, Nobuyuki; Yamaya, Tomoyuki. ACT domain repeat protein 7, ACR7, interacts with a chaperone HSP18.0-CII in rice nuclei. Plant & Cell Physiology. 2006;47(7):891-904.  PubMed
Comments: Used in: IP
Tanaka, Tsubasa; Kato, Yasuko; Matsuda, Kazuki; Hanyu-Nakamura, Kazuko; Nakamura, Akira. Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm. Development (Cambridge, England). 2011;138(12):2523-32.  PubMed
Comments: Used in: IP
Shirae-Kurabayashi, Maki; Matsuda, Kazuki; Nakamura, Akira. Ci-Pem-1 localizes to the nucleus and represses somatic gene transcription in the germline of Ciona intestinalis embryos. Development (Cambridge, England). 2011;138(14):2871-81.  PubMed
Comments: Used in: IP
Zhong, Yongwang; Wang, Yang; Yang, Hui; Ballar, Petek; Lee, Jin-gu; Ye, Yihong; Monteiro, Mervyn J; Fang, Shengyun. Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin. The Journal Of Biological Chemistry. 2011;286(39):33921-30.  PubMed
Comments: Used in: IP
Cai, Liquan; Phong, Binh L; Fisher, Alfred L; Wang, Zhou. Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes. The Journal Of Biological Chemistry. 2011;286(41):35915-35921.  PubMed
Comments: Used in: IP
Roberts, Diana; Pedmale, Ullas V; Morrow, Johanna; Sachdev, Shrikesh; Lechner, Esther; Tang, Xiaobo; Zheng, Ning; Hannink, Mark; Genschik, Pascal; Liscum, Emmanuel. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3). The Plant Cell. 2011;23(10):3627-40.  PubMed
Comments: Used in: IP
Sasabe, Michiko; Boudolf, Véronique; De Veylder, Lieven; Inzé, Dirk; Genschik, Pascal; Machida, Yasunori. Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2011;108(43):17844-9.  PubMed
Comments: Used in: Co-IP
Fukumoto, Yuri; Kurita, Souichi; Takai, Yoshimi; Ogita, Hisakazu. Role of scaffold protein afadin dilute domain-interacting protein (ADIP) in platelet-derived growth factor-induced cell movement by activating Rac protein through Vav2 protein. The Journal Of Biological Chemistry. 2011;286(50):43537-48.  PubMed
Comments: Used in: Co-IP
Aung, Kyaw; Hu, Jianping. The Arabidopsis tail-anchored protein PEROXISOMAL AND MITOCHONDRIAL DIVISION FACTOR1 is involved in the morphogenesis and proliferation of peroxisomes and mitochondria. The Plant Cell. 2011;23(12):4446-61.  PubMed
Comments: Used in: Co-IP
Heyman, Jefri; Van den Daele, Hilde; De Wit, Kevin; Boudolf, Véronique; Berckmans, Barbara; Verkest, Aurine; Alvim Kamei, Claire Lessa; De Jaeger, Geert; Koncz, Csaba; De Veylder, Lieven. Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. The Plant Cell. 2011;23(12):4394-410.  PubMed
Comments: Used in: Co-IP
Henderson, Mark J; Richie, Christopher T; Airavaara, Mikko; Wang, Yun; Harvey, Brandon K. Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors. The Journal Of Biological Chemistry. 2013;288(6):4209-25.  PubMed
Comments: Used in: IP
Paster, Wolfgang; Brockmeyer, Claudia; Fu, Guo; Simister, Philip C; de Wet, Ben; Martinez-Riaño, Ana; Hoerter, John A H; Feller, Stephan M; Wülfing, Christoph; Gascoigne, Nicholas R J; Acuto, Oreste. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal Of Immunology (Baltimore, Md. : 1950). 2013;190(7):3749-56.  PubMed
Comments: Used in: IP
Celesnik, Helena; Ali, Gul S; Robison, Faith M; Reddy, Anireddy S N. Arabidopsis thaliana VOZ (Vascular plant One-Zinc finger) transcription factors are required for proper regulation of flowering time. Biology Open. 2013;2(4):424-31.  PubMed
Comments: Used in: ChIP
He, Minghao; Zhang, Linlin; Wang, Xincheng; Huo, Lihong; Sun, Lei; Feng, Chengye; Jing, Xutian; Du, Danyao; Liang, Huabin; Liu, Min; Hong, Zhangyong; Zhou, Jun. Systematic Analysis of the Functions of Lysine Acetylation in the Regulation of Tat Activity. Plos One. 8(6):e67186.  PubMed
Comments: Used in: IP
Choi, Hoon-In; Chung, Kyoung-Jin; Yang, Hee-Young; Ren, Lina; Sohn, Sungoh; Kim, Poo-Reun; Kook, Min-Suk; Choy, Hyon E; Lee, Tae-Hoon. Peroxiredoxin V selectively regulates IL-6 production by modulating the Jak2-Stat5 pathway. Free Radical Biology & Medicine. 2013;65:270-279.  PubMed
Comments: Used in: IP
Liu, Qi; Yan, Qingqing; Liu, Yin; Hong, Fang; Sun, Zhenfei; Shi, Leilei; Huang, Ying; Fang, Yuda. Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies. Plant Physiology. 2013;163(1):108-17.  PubMed
Comments: Used in: Co-IP
Tomida, Junya; Takata, Kei-ichi; Lange, Sabine S; Schibler, Andria C; Yousefzadeh, Matthew J; Bhetawal, Sarita; Dent, Sharon Y R; Wood, Richard D. REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ. Nucleic Acids Research. 2015;43(2):1000-11.  PubMed
Comments: Used in: IP
Underhill, Suzanne M; Wheeler, David S; Amara, Susan G. Differential regulation of two isoforms of the glial glutamate transporter EAAT2 by DLG1 and CaMKII. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience. 2015;35(13):5260-70.  PubMed
Comments: Used in: IP
Okatsu, Kei; Koyano, Fumika; Kimura, Mayumi; Kosako, Hidetaka; Saeki, Yasushi; Tanaka, Keiji; Matsuda, Noriyuki. Phosphorylated ubiquitin chain is the genuine Parkin receptor. The Journal Of Cell Biology. 2015;209(1):111-28.  PubMed
Comments: Used in: IP
Hodder, Anthony N; Sleebs, Brad E; Czabotar, Peter E; Gazdik, Michelle; Xu, Yibin; O'Neill, Matthew T; Lopaticki, Sash; Nebl, Thomas; Triglia, Tony; Smith, Brian J; Lowes, Kym; Boddey, Justin A; Cowman, Alan F. Structural basis for plasmepsin V inhibition that blocks export of malaria proteins to human erythrocytes. Nature Structural & Molecular Biology. 2015;22(8):590-6.  PubMed
Comments: Used in: IP
Wang, Dan; Nguyen, Minh M; Masoodi, Khalid Z; Singh, Prabhpreet; Jing, Yifeng; O'Malley, Katherine; Dar, Javid A; Dhir, Rajiv; Wang, Zhou. Splicing Factor Prp8 Interacts With NES(AR) and Regulates Androgen Receptor in Prostate Cancer Cells. Molecular Endocrinology (Baltimore, Md.). 2015;29(12):1731-42.  PubMed
Comments: Used in: IP
Tatavosian, Roubina; Zhen, Chao Yu; Duc, Huy Nguyen; Balas, Maggie M; Johnson, Aaron M; Ren, Xiaojun. Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging. The Journal Of Biological Chemistry. 2015;290(47):28038-28054.  PubMed
Comments: Used in: IP
Boddey, Justin A; O'Neill, Matthew T; Lopaticki, Sash; Carvalho, Teresa G; Hodder, Anthony N; Nebl, Thomas; Wawra, Stephan; van West, Pieter; Ebrahimzadeh, Zeinab; Richard, Dave; Flemming, Sven; Spielmann, Tobias; Przyborski, Jude; Babon, Jeff J; Cowman, Alan F. Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding. Nature Communications. 2016;7:10470.  PubMed
Comments: Used in: IP
Nadarajan, Saravanapriah; Mohideen, Firaz; Tzur, Yonatan B; Ferrandiz, Nuria; Crawley, Oliver; Montoya, Alex; Faull, Peter; Snijders, Ambrosius P; Cutillas, Pedro R; Jambhekar, Ashwini; Blower, Michael D; Martinez-Perez, Enrique; Harper, J Wade; Colaiacovo, Monica P. The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis. Elife. 2016;5:e12039.  PubMed
Comments: Used in: IP
Liu, Jie; Cheng, Xiliu; Liu, Pan; Li, Dayong; Chen, Tao; Gu, Xiaofeng; Sun, Jiaqiang. MicroRNA319-regulated TCPs interact with FBHs and PFT1 to activate CO transcription and control flowering time in Arabidopsis. Plos Genetics. 2017;13(5):e1006833.  PubMed
Comments: Used in: Co-IP
Brown, Kristen C; Svendsen, Joshua M; Tucci, Rachel M; Montgomery, Brooke E; Montgomery, Taiowa A. ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline. Nucleic Acids Research. 2017;45(15):9093-9107.  PubMed
Comments: Used in: IP
Niida, Hiroyuki; Matsunuma, Ryoichi; Horiguchi, Ryo; Uchida, Chiharu; Nakazawa, Yuka; Motegi, Akira; Nishimoto, Koji; Sakai, Satoshi; Ohhata, Tatsuya; Kitagawa, Kyoko; Moriwaki, Shinichi; Nishitani, Hideo; Ui, Ayako; Ogi, Tomoo; Kitagawa, Masatoshi. Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair. Nature Communications. 2017;8:16102.  PubMed
Comments: Used in: IP
Dong, Jie; Ni, Weimin; Yu, Renbo; Deng, Xing Wang; Chen, Haodong; Wei, Ning. Light-Dependent Degradation of PIF3 by SCF(EBF1/2) Promotes a Photomorphogenic Response in Arabidopsis. Current Biology : Cb. 2017;27(16):2420-2430.e6.  PubMed
Comments: Used in: Co-IP
Song, Xiaoguang; Lu, Zefu; Yu, Hong; Shao, Gaoneng; Xiong, Jinsong; Meng, Xiangbing; Jing, Yanhui; Liu, Guifu; Xiong, Guosheng; Duan, Jingbo; Yao, Xue-Feng; Liu, Chun-Ming; Li, Hongqing; Wang, Yonghong; Li, Jiayang. IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice. Cell Research. 2017;27(9):1128-1141.  PubMed
Comments: Used in: Co-IP
Wang, Sheng; Yin, Bin; Li, Haoyang; Xiao, Bang; Lǚ, Kai; Feng, Chiguang; He, Jianguo; Li, Chaozheng. MKK4 from Litopenaeus vannamei is a regulator of p38 MAPK kinase and involved in anti-bacterial response. Developmental And Comparative Immunology. 2018;78:61-70.  PubMed
Comments: Used in: Co-IP
Lüningschrör, Patrick; Binotti, Beyenech; Dombert, Benjamin; Heimann, Peter; Perez-Lara, Angel; Slotta, Carsten; Thau-Habermann, Nadine; R von Collenberg, Cora; Karl, Franziska; Damme, Markus; Horowitz, Arie; Maystadt, Isabelle; Füchtbauer, Annette; Füchtbauer, Ernst-Martin; Jablonka, Sibylle; Blum, Robert; Üçeyler, Nurcan; Petri, Susanne; Kaltschmidt, Barbara; Jahn, Reinhard; Kaltschmidt, Christian; Sendtner, Michael. Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nature Communications. 2017;8(1):678.  PubMed
Comments: Used in: IP
Yahara, Masao; Kitamura, Akira; Kinjo, Masataka. U6 snRNA expression prevents toxicity in TDP-43-knockdown cells. Plos One. 12(11):e0187813.  PubMed
Kageyama, Shun; Saito, Tetsuya; Obata, Miki; Koide, Ryo-Hei; Ichimura, Yoshinobu; Komatsu, Masaaki. Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant. Molecular And Cellular Biology. 2018;38(7)  PubMed
Comments: Used in: IP
Tomida, Junya; Takata, Kei-Ichi; Bhetawal, Sarita; Person, Maria D; Chao, Hsueh-Ping; Tang, Dean G; Wood, Richard D. FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. The Embo Journal. 2018;37(12)  PubMed
Comments: Used in: IP
Yu, Xiaoming; Meng, Xinchao; Liu, Yutong; Li, Ning; Zhang, Ai; Wang, Tian-Jing; Jiang, Lili; Pang, Jinsong; Zhao, Xinxin; Qi, Xin; Zhang, Meishan; Wang, Shucai; Liu, Bao; Xu, Zheng-Yi. The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize. Plant Molecular Biology. 2018;97(4-5):451-465.  PubMed
Comments: Used in: ChIP
Yu, Xiaoming; Meng, Xinchao; Liu, Yutong; Li, Ning; Zhang, Ai; Wang, Tian-Jing; Jiang, Lili; Pang, Jinsong; Zhao, Xinxin; Qi, Xin; Zhang, Meishan; Wang, Shucai; Liu, Bao; Xu, Zheng-Yi. The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize. Plant Molecular Biology. 2018;97(4-5):451-465.  PubMed
Comments: Used in: Co-IP
Xu, Zhiwei; Isaji, Tomoya; Fukuda, Tomohiko; Wang, Yuqin; Gu, Jianguo. O-GlcNAcylation regulates integrin-mediated cell adhesion and migration via formation of focal adhesion complexes. The Journal Of Biological Chemistry. 2019;294(9):3117-3124.  PubMed
Comments: Used in: IP
Yu, Xiaoming; Meng, Xinchao; Liu, Yutong; Wang, Xutong; Wang, Tian-Jing; Zhang, Ai; Li, Ning; Qi, Xin; Liu, Bao; Xu, Zheng-Yi. The chromatin remodeler ZmCHB101 impacts alternative splicing contexts in response to osmotic stress. Plant Cell Reports. 2019;38(2):131-145.  PubMed
Comments: Used in: Co-IP
Yu, Xiaoming; Meng, Xinchao; Liu, Yutong; Wang, Xutong; Wang, Tian-Jing; Zhang, Ai; Li, Ning; Qi, Xin; Liu, Bao; Xu, Zheng-Yi. The chromatin remodeler ZmCHB101 impacts alternative splicing contexts in response to osmotic stress. Plant Cell Reports. 2019;38(2):131-145.  PubMed
Comments: Used in: ChIP
Wang, Huan; Zou, Shenghao; Li, Yiwen; Lin, Fanyun; Tang, Dingzhong. An ankyrin-repeat and WRKY-domain-containing immune receptor confers stripe rust resistance in wheat. Nature Communications. 2020;11(1):1353.  PubMed
Comments: Used in: IP
Manage, Kevin I; Rogers, Alicia K; Wallis, Dylan C; Uebel, Celja J; Anderson, Dorian C; Nguyen, Dieu An H; Arca, Katerina; Brown, Kristen C; Cordeiro Rodrigues, Ricardo J; de Albuquerque, Bruno Fm; Ketting, René F; Montgomery, Taiowa A; Phillips, Carolyn Marie. A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans. Elife. 2020;9  PubMed
Bisi, Sara; Marchesi, Stefano; Rizvi, Abrar; Carra, Davide; Beznoussenko, Galina V; Ferrara, Ines; Deflorian, Gianluca; Mironov, Alexander; Bertalot, Giovanni; Pisati, Federica; Oldani, Amanda; Cattaneo, Angela; Saberamoli, Ghazaleh; Pece, Salvatore; Viale, Giuseppe; Bachi, Angela; Tripodo, Claudio; Scita, Giorgio; Disanza, Andrea. IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules. Nature Communications. 2020;11(1):3516.  PubMed
Comments: Used in: IP