RIP-CERTIFIED ANTIBODY:
Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay Kit distributed from MBL. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.
| Product Specifications | |
| Reactivity | Human |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Peptide bond synthesis GEELLPPESTPIPANLS KLH (480-496 aa) |
| Gene ID - Human | |
| Gene ID - Mouse | |
| Formulation | 200 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Citations for Anti-eIF4G1 (Human) pAb – 2 Found |
| Sundararaman, Balaji; Zhan, Lijun; Blue, Steven M; Stanton, Rebecca; Elkins, Keri; Olson, Sara; Wei, Xintao; Van Nostrand, Eric L; Pratt, Gabriel A; Huelga, Stephanie C; Smalec, Brendan M; Wang, Xiaofeng; Hong, Eurie L; Davidson, Jean M; Lécuyer, Eric; Graveley, Brenton R; Yeo, Gene W. Resources for the Comprehensive Discovery of Functional RNA Elements. Molecular Cell. 2016;61(6):903-13. PubMed - Comments: Used in: ICC |
| Rissland, Olivia S; Subtelny, Alexander O; Wang, Miranda; Lugowski, Andrew; Nicholson, Beth; Laver, John D; Sidhu, Sachdev S; Smibert, Craig A; Lipshitz, Howard D; Bartel, David P. The influence of microRNAs and poly(A) tail length on endogenous mRNA-protein complexes. Genome Biology. 2017;18(1):211. PubMed - Comments: Used in: RIP |