Monoclonal Antibody of 100 μg targeting EGF-R for FCM, ICC, IP, WB.
| Product Specifications | |
| Application | FC, ICC, IP, WB |
| Reactivity | Human |
| Clonality | Monoclonal IgG2b ĸ |
| Host | Mouse |
| Immunogen | Intracellular domain Recombinant human EGF-R (648-1186 aa) |
| Gene ID - Human | |
| Gene ID - Mouse | |
| Formulation | The protein concentration should be 1 mg/mL and the IgG concentration should be 100 μg/mL in PBS 50% glycerol, pH 7.2. No preservatives are contained. |
| Citations for Anti-EGF-R (Human) mAb (Clone: 6F1) – 23 Found |
| Lazarovici, P; Oshima, M; Shavit, D; Shibutani, M; Jiang, H; Monshipouri, M; Fink, D; Movsesyan, V; Guroff, G. Down-regulation of epidermal growth factor receptors by nerve growth factor in PC12 cells is p140(trk)-, Ras-, and Src-dependent. The Journal Of Biological Chemistry. 1997;272(17):11026-34. PubMed |
| Ichiba, T; Kuraishi, Y; Sakai, O; Nagata, S; Groffen, J; Kurata, T; Hattori, S; Matsuda, M. Enhancement of guanine-nucleotide exchange activity of C3G for Rap1 by the expression of Crk, CrkL, and Grb2. The Journal Of Biological Chemistry. 1997;272(35):22215-20. PubMed - Comments: Used in: WB |
| Shibutani, M; Lazarovici, P; Johnson, A C; Katagiri, Y; Guroff, G. Transcriptional down-regulation of epidermal growth factor receptors by nerve growth factor treatment of PC12 cells. The Journal Of Biological Chemistry. 1998;273(12):6878-84. PubMed - Comments: Used in: WB |
| Hashimoto, Y; Katayama, H; Kiyokawa, E; Ota, S; Kurata, T; Gotoh, N; Otsuka, N; Shibata, M; Matsuda, M. Phosphorylation of CrkII adaptor protein at tyrosine 221 by epidermal growth factor receptor. The Journal Of Biological Chemistry. 1998;273(27):17186-91. PubMed - Comments: Used in: IP |
| Lazarovici, P; Jiang, H; Fink, D Jr. The 38-amino-acid form of pituitary adenylate cyclase-activating polypeptide induces neurite outgrowth in PC12 cells that is dependent on protein kinase C and extracellular signal-regulated kinase but not on protein kinase A, nerve growth factor receptor tyrosine kinase, p21(ras) G protein, and pp60(c-src) cytoplasmic tyrosine kinase. Molecular Pharmacology. 1998;54(3):547-58. PubMed - Comments: Used in: WB |
| Liu, X W; Gong, L J; Guo, L Y; Katagiri, Y; Jiang, H; Wang, Z Y; Johnson, A C; Guroff, G. The Wilms' tumor gene product WT1 mediates the down-regulation of the rat epidermal growth factor receptor by nerve growth factor in PC12 cells. The Journal Of Biological Chemistry. 2001;276(7):5068-73. PubMed - Comments: Used in: WB |
| Liu, X W; Gong, L J; Guo, L Y; Katagiri, Y; Jiang, H; Wang, Z Y; Johnson, A C; Guroff, G. The Wilms' tumor gene product WT1 mediates the down-regulation of the rat epidermal growth factor receptor by nerve growth factor in PC12 cells. The Journal Of Biological Chemistry. 2001;276(7):5068-73. PubMed - Comments: Used in: ICC |
| Nakamura, Takeshi; Komiya, Misako; Sone, Kiyoaki; Hirose, Eiji; Gotoh, Noriko; Morii, Hiroshi; Ohta, Yasutaka; Mori, Nozomu. Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules. Molecular And Cellular Biology. 2002;22(24):8721-34. PubMed - Comments: Used in: ICC |
| Mizuno, Emi; Kawahata, Kensuke; Kato, Masaki; Kitamura, Naomi; Komada, Masayuki. STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif. Molecular Biology Of The Cell. 2003;14(9):3675-89. PubMed - Comments: Used in: ICC |
| Mizuno, Emi; Iura, Takanobu; Mukai, Akiko; Yoshimori, Tamotsu; Kitamura, Naomi; Komada, Masayuki. Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes. Molecular Biology Of The Cell. 2005;16(11):5163-74. PubMed - Comments: Used in: WB |
| Mizuno, Emi; Iura, Takanobu; Mukai, Akiko; Yoshimori, Tamotsu; Kitamura, Naomi; Komada, Masayuki. Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes. Molecular Biology Of The Cell. 2005;16(11):5163-74. PubMed - Comments: Used in: IP |
| Mizuno, Emi; Iura, Takanobu; Mukai, Akiko; Yoshimori, Tamotsu; Kitamura, Naomi; Komada, Masayuki. Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes. Molecular Biology Of The Cell. 2005;16(11):5163-74. PubMed - Comments: Used in: ICC |
| Yamasaki, Akinori; Tani, Katsuko; Yamamoto, Akitsugu; Kitamura, Naomi; Komada, Masayuki. The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A. Molecular Biology Of The Cell. 2006;17(11):4876-87. PubMed - Comments: Used in: WB |
| Chung, Inhee; Akita, Robert; Vandlen, Richard; Toomre, Derek; Schlessinger, Joseph; Mellman, Ira. Spatial control of EGF receptor activation by reversible dimerization on living cells. Nature. 2010;464(7289):783-7. PubMed - Comments: Used in: WB |
| Ishikawa, Kouki; Nara, Atsuki; Matsumoto, Kunihiro; Hanafusa, Hiroshi. EGFR-dependent phosphorylation of leucine-rich repeat kinase LRRK1 is important for proper endosomal trafficking of EGFR. Molecular Biology Of The Cell. 2012;23(7):1294-306. PubMed |
| Spiess, Christoph; Merchant, Mark; Huang, Arthur; Zheng, Zhong; Yang, Nai-Ying; Peng, Jing; Ellerman, Diego; Shatz, Whitney; Reilly, Dorothea; Yansura, Daniel G; Scheer, Justin M. Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies. Nature Biotechnology. 2013;31(8):753-8. PubMed - Comments: Used in: WB |
| Hasegawa, Junya; Iwamoto, Ryo; Otomo, Takanobu; Nezu, Akiko; Hamasaki, Maho; Yoshimori, Tamotsu. Autophagosome-lysosome fusion in neurons requires INPP5E, a protein associated with Joubert syndrome. The Embo Journal. 2016;35(17):1853-67. PubMed - Comments: Used in: WB |
| Kondo, Hidehito; Maksimova, Nadezda; Otomo, Takanobu; Kato, Hisakazu; Imai, Atsuko; Asano, Yoshihiro; Kobayashi, Kaori; Nojima, Satoshi; Nakaya, Akihiro; Hamada, Yusuke; Irahara, Kaori; Gurinova, Elizaveta; Sukhomyasova, Aitalina; Nogovicina, Anna; Savvina, Mira; Yoshimori, Tamotsu; Ozono, Keiichi; Sakai, Norio. Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms. Human Molecular Genetics. 2017;26(1):173-183. PubMed - Comments: Used in: WB |
| Hori, Ikumi; Otomo, Takanobu; Nakashima, Mitsuko; Miya, Fuyuki; Negishi, Yutaka; Shiraishi, Hideaki; Nonoda, Yutaka; Magara, Shinichi; Tohyama, Jun; Okamoto, Nobuhiko; Kumagai, Takeshi; Shimoda, Konomi; Yukitake, Yoshiya; Kajikawa, Daigo; Morio, Tomohiro; Hattori, Ayako; Nakagawa, Motoo; Ando, Naoki; Nishino, Ichizo; Kato, Mitsuhiro; Tsunoda, Tatsuhiko; Saitsu, Hirotomo; Kanemura, Yonehiro; Yamasaki, Mami; Kosaki, Kenjiro; Matsumoto, Naomichi; Yoshimori, Tamotsu; Saitoh, Shinji. Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement. Scientific Reports. 2017;7(1):3552. PubMed - Comments: Used in: WB |
| Muta, Yu; Fujita, Yoshihisa; Sumiyama, Kenta; Sakurai, Atsuro; Taketo, M Mark; Chiba, Tsutomu; Seno, Hiroshi; Aoki, Kazuhiro; Matsuda, Michiyuki; Imajo, Masamichi. Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine. Nature Communications. 2018;9(1):2174. PubMed - Comments: Used in: FCM |
| Hara, Takato; Tatsuishi, Hiroko; Banno, Tomomi; Fujie, Tomoya; Yamamoto, Chika; Naka, Hiroshi; Kaji, Toshiyuki. Copper(II) Bis(diethyldithiocarbamate) Induces the Expression of Syndecan-4, a Transmembrane Heparan Sulfate Proteoglycan, via p38 MAPK Activation in Vascular Endothelial Cells. International Journal Of Molecular Sciences. 2018;19(11) PubMed - Comments: Used in: IHC |
| Yamanaka, Shunpei; Suzuki, Susumu; Ito, Hideaki; Sivasundaram, Karnan; Hanamura, Ichiro; Okubo, Ikuko; Yoshikawa, Kazuhiro; Ono, Shoya; Takahara, Taishi; Satou, Akira; Tsuzuki, Toyonori; Ueda, Ryuzo; Ogawa, Tetsuya; Fujimoto, Yasushi. Establishment of Mucoepidermoid Carcinoma Cell Lines from Surgical and Recurrence Biopsy Specimens. International Journal Of Molecular Sciences. 2023;24(2) PubMed - Comments: Used in: WB |
| Kondo, Yutaro; Suzuki, Susumu; Ono, Shoya; Goto, Mitsuo; Miyabe, Satoru; Ogawa, Tetsuya; Tsuchida, Hiromi; Ito, Hideaki; Takahara, Taishi; Satou, Akira; Tsuzuki, Toyonori; Yoshikawa, Kazuhiro; Ueda, Ryuzo; Nagao, Toru. In Situ PD-L1 Expression in Oral Squamous Cell Carcinoma Is Induced by Heterogeneous Mechanisms among Patients. International Journal Of Molecular Sciences. 2022;23(8) PubMed - Comments: Used in: WB |