Anti-Cyclobutane Pyrimidine Dimers (CPDs) mAb (Clone TDM-2)

Cosmo Bio LTD

Catalog No: CAC-NM-DND-001

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Catalog numbers beginning with "CAC" represent antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody Dashboard to browse the CAC antibody list, organized by research topic.

Cyclobutane pyrimidine dimer (CPD)-specific mAb TDM-2 was cloned by fusing mouse myeloma cells with splenocytes from BALB/c mice immunized with methylated BSA conjugated with UVC-irradiated calf thymus DNA. TDM-2 binds CPDs in denatured DNA from all organisms from bacteria to human and is validated for ELISA and indirect immunofluorescence.

Background

DNA damage in cells exposed to ultraviolet (UV) radiation plays significant roles in cell-cycle arrest, activation of DNA repair, cell killing, mutation, and neoplastic transformation. The major types of DNA damage induced by UVB (280-315 nm, component of sunlight) and by UVC (200-280 nm) are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), which are formed between adjacent pyrimidine nucleotides on the same strand of DNA. Approximately 70-80% of UV-induced DNA damage is CPDs and the remainder is 6-4PPs and Dewar isomers of 6-4PPs. In normal human cells, these types of DNA lesions are repaired by the nucleotide excision repair system.

(19) have established monoclonal antibodies specific for CPDs and 6-4PPs. These antibodies enable quantitation of photoproducts in DNA purified from cultured cells or from the skin epidermis by ELISA and to visualize and measure photoproducts in DNA in cultured cells or the skin by indirect immunofluorescence (IIF). This technology will contribute to studies of molecular mechanisms of cellular responses to UV and DNA damage in many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetology.

Reactivity

• CPDs in single-stranded DNA
• CPDs formed in every dipyrimidine sequence (TT, TC, CT and CC)
• CPDs formed in oligonucleotides consisting of more than eight bases

Applications

• Immunocytochemistry            1:1500
• ELISA                                     1:1000

Source

The CPD-specific hybridoma (clone TDM-2) was established by fusion of mouse myeloma cells with splenocytes from BALB/c mice immunized with methylated BSA conjugated with UVC-irradiated calf thymus DNA. Hybridoma culture supernatant was collected and precipitated with ice-cold ammonium sulfate. After centrifugation, the pellet, dissolved in small volume of double-distilled water, was dialyzed against PBS and lyophilized.

Formulation

• Lyophilate to be reconstituted in 100 ml of distilled water
• Contains no preservative

Storage

• Lyophilized form        -20°C
• Reconstituted form     -20°C
• Stable for at least 1 year; aliquot to avoid freezing and thawing

Citations for Anti-Cyclobutane Pyrimidine Dimers (CPDs) mAb (Clone TDM-2) – 49 Found

Jarrett, Stuart G; Wolf Horrell, Erin M; D'Orazio, John A. AKAP12 mediates PKA-induced phosphorylation of ATR to enhance nucleotide excision repair. Nucleic Acids Research. 2016;44(22):10711-10726. 

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Iwabuchi, Kosei; Hidema, Jun; Tamura, Kentaro; Takagi, Shingo; Hara-Nishimura, Ikuko. Plant Nuclei Move to Escape Ultraviolet-Induced DNA Damage and Cell Death. Plant Physiology. 2016;170(2):678-85. 

Falcone Ferreyra, María Lorena; Casadevall, Romina; D'Andrea, Lucio; AbdElgawad, Hamada; Beemster, Gerrit T S; Casati, Paula. AtPDCD5 Plays a Role in Programmed Cell Death after UV-B Exposure in Arabidopsis. Plant Physiology. 2016;170(4):2444-60. 

Gracheva, Ekaterina; Chitale, Shalaka; Wilhelm, Thomas; Rapp, Alexander; Byrne, Jonathan; Stadler, Jens; Medina, Rebeca; Cardoso, M Cristina; Richly, Holger. ZRF1 mediates remodeling of E3 ligases at DNA lesion sites during nucleotide excision repair. The Journal Of Cell Biology. 2016;213(2):185-200. 

Canturk, Fazile; Karaman, Muhammet; Selby, Christopher P; Kemp, Michael G; Kulaksiz-Erkmen, Gulnihal; Hu, Jinchuan; Li, Wentao; Lindsey-Boltz, Laura A; Sancar, Aziz. Nucleotide excision repair by dual incisions in plants. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2016;113(17):4706-10. 

Schuch, André P; Lipinski, Victor M; Santos, Mauricio B; Santos, Caroline P; Jardim, Sinara S; Cechin, Sonia Z; Loreto, Elgion L S. Molecular and sensory mechanisms to mitigate sunlight-induced DNA damage in treefrog tadpoles. The Journal Of Experimental Biology. 2015;218(Pt 19):3059-67. 

Lario, Luciana Daniela; Botta, Pablo; Casati, Paula; Spampinato, Claudia Patricia. Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination. Journal Of Experimental Botany. 2015;66(11):3019-26. 

Weems, Juston C; Slaughter, Brian D; Unruh, Jay R; Hall, Shawn M; McLaird, Merry B; Gilmore, Joshua M; Washburn, Michael P; Florens, Laurence; Yasukawa, Takashi; Aso, Teijiro; Conaway, Joan W; Conaway, Ronald C. Assembly of the Elongin A Ubiquitin Ligase Is Regulated by Genotoxic and Other Stresses. The Journal Of Biological Chemistry. 2015;290(24):15030-41. 

Matsumoto, Syota; Fischer, Eric S; Yasuda, Takeshi; Dohmae, Naoshi; Iwai, Shigenori; Mori, Toshio; Nishi, Ryotaro; Yoshino, Ken-ichi; Sakai, Wataru; Hanaoka, Fumio; Thomä, Nicolas H; Sugasawa, Kaoru. Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein. Nucleic Acids Research. 2015;43(3):1700-13. 

Harberts, Erin; Zhou, Hua; Fishelevich, Rita; Liu, Juan; Gaspari, Anthony A. Ultraviolet radiation signaling through TLR4/MyD88 constrains DNA repair and plays a role in cutaneous immunosuppression. Journal Of Immunology (Baltimore, Md. : 1950). 2015;194(7):3127-35. 

Lario, Luciana Daniela; Botta, Pablo; Casati, Paula; Spampinato, Claudia Patricia. Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination. Journal Of Experimental Botany. 2015;66(11):3019-26. 

Peacock, Matthew; Brem, Reto; Macpherson, Peter; Karran, Peter. DNA repair inhibition by UVA photoactivated fluoroquinolones and vemurafenib. Nucleic Acids Research. 2014;42(22):13714-22. 

Hill, Sarah J; Clark, Allison P; Silver, Daniel P; Livingston, David M. BRCA1 pathway function in basal-like breast cancer cells. Molecular And Cellular Biology. 2014;34(20):3828-42. 

Kim, Hyungjin; Dejsuphong, Donniphat; Adelmant, Guillaume; Ceccaldi, Raphael; Yang, Kailin; Marto, Jarrod A; D'Andrea, Alan D. Transcriptional repressor ZBTB1 promotes chromatin remodeling and translesion DNA synthesis. Molecular Cell. 2014;54(1):107-118. 

Liu, Hannah; Tuchinda, Papapit; Fishelevich, Rita; Harberts, Erin; Gaspari, Anthony A. Human in vitro skin organ culture as a model system for evaluating DNA repair. Journal Of Dermatological Science. 2014;74(3):236-41. 

Wolters, Stefanie; Ermolaeva, Maria A; Bickel, Jeremy S; Fingerhut, Jaclyn M; Khanikar, Jayshree; Chan, Raymond C; Schumacher, Björn. Loss of Caenorhabditis elegans BRCA1 promotes genome stability during replication in smc-5 mutants. Genetics. 2014;196(4):985-99. 

Lee, Ming-Hsiang; Wang, Liya; Chang, Zee-Fen. The contribution of mitochondrial thymidylate synthesis in preventing the nuclear genome stress. Nucleic Acids Research. 2014;42(8):4972-84. 

Biswas, Anup Kumar; Mitchell, David L; Johnson, David G. E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis. Cancer Research. 2014;74(12):3369-77. 

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Reef, Ruth; Dunn, Simon; Levy, Oren; Dove, Sophie; Shemesh, Eli; Brickner, Itzchak; Leggat, William; Hoegh-Guldberg, Ove. Photoreactivation is the main repair pathway for UV-induced DNA damage in coral planulae. The Journal Of Experimental Biology. 2009;212(17):2760-6. 

Despras, Emmanuelle; Daboussi, Fayza; Hyrien, Olivier; Marheineke, Kathrin; Kannouche, Patricia L. ATR/Chk1 pathway is essential for resumption of DNA synthesis and cell survival in UV-irradiated XP variant cells. Human Molecular Genetics. 2010;19(9):1690-701. 

Cherubini, Gioia; Naim, Valeria; Caruso, Paola; Burla, Romina; Bogliolo, Massimo; Cundari, Enrico; Benihoud, Karim; Saggio, Isabella; Rosselli, Filippo. The FANC pathway is activated by adenovirus infection and promotes viral replication-dependent recombination. Nucleic Acids Research. 2011;39(13):5459-73. 

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Ming, Mei; Shea, Christopher R; Guo, Xiumei; Li, Xiaoling; Soltani, Keyoumars; Han, Weinong; He, Yu-Ying. Regulation of global genome nucleotide excision repair by SIRT1 through xeroderma pigmentosum C. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2010;107(52):22623-8. 

Han, Weinong; Ming, Mei; He, Yu-Ying. Caffeine promotes ultraviolet B-induced apoptosis in human keratinocytes without complete DNA repair. The Journal Of Biological Chemistry. 2011;286(26):22825-32. 

Filip, Adriana; Daicoviciu, Doina; Clichici, Simona; Bolfa, Pompei; Catoi, Cornel; Baldea, Ioana; Bolojan, Laura; Olteanu, Diana; Muresan, Adriana; Postescu, I D. The effects of grape seeds polyphenols on SKH-1 mice skin irradiated with multiple doses of UV-B. Journal Of Photochemistry And Photobiology. B, Biology. 2011;105(2):133-42. 

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Fina, Julieta P; Casati, Paula. HAG3, a Histone Acetyltransferase, Affects UV-B Responses by Negatively Regulating the Expression of DNA Repair Enzymes and Sunscreen Content in Arabidopsis thaliana. Plant & Cell Physiology. 2015;56(7):1388-400. 

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Dubey, Divya; Srivastav, Ajeet K; Singh, Jyoti; Chopra, Deepti; Qureshi, Saba; Kushwaha, Hari Narayan; Singh, Nivedita; Ray, Ratan Singh. Photoexcited triclosan induced DNA damage and oxidative stress via p38 MAP kinase signaling involving type I radicals under sunlight/UVB exposure. Ecotoxicology And Environmental Safety. 2019;174( 30844667):270-282. 

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