Anti 6-Sulfated Unsaturated Disaccharide Neoepitopes (C-6-S "stubs") of Chondroitin Sulfate mAb (Clone 3B3)

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Catalog No: CAC-PRPG-BC-M04

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Monoclonal antibody 3B3 recognizes 6-sulfated unsaturated disaccharide neoepitopes (i.e. C-6-S "stubs") generated at the non-reducing terminal of Chondroitin Sulfate glycosaminoglycan chains that have been pre-digested with either Chondroitinase ABC or Chondroitinase ACII. Monoclonal antibody 3B3 also recognizes a non-reducing end saturated disaccharide epitope in 'native' Chondroitin Sulfate glycosaminoglycan chains consisting of a terminal glucuronic acid adjacent to 6-sulfated N-acetyl-galactosamine. The chondroitinase-generated neoepitope is often denoted as 3B3(+) and the 'native' terminal epitope as 3B3(-) in publications [see Figure 2; Caterson B (2012) Int. J. Exp. Pathol. 93: 1 - 10].

References:
1) Schwend T, Deaton RJ, Zhang Y, Caterson B & Conrad CW (2012). Corneal sulphated glycosaminoglycans and their effects on trigeminal nerve growth cone behaviour in vitro – roles for ECM in corneal innervation. Invest Opthamol Vis Sci. 53:
2) Caterson B. (2012). Chondroitin sulphate glycosaminoglycans: fun for some and confusion for others. Int. J. of Exp. Path. 93: 1 – 10 PubMed: 22264297
3) Liles M, Palka BP, Harris A,Kerr BC, Hughes CE, Young RD, Meek KM, Caterson B, Quantok AJ (2010). Differential relative sulphation of keratan sulphate glycosaminoglycan in the chick cornea during embryonic development. Invest. Opthalmol. Vis. Sci. 51: 1365-1372 PubMed: 19815728
4) Davies L, Blain E, Caterson B and Duance VC (2008). Chondroitin sulphate impedes the migration of a sub-population of articular cartilage chondrocytes. Osteoarthritis & Cartilage 16: 855 - 864 PubMed: 18222711
5) Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemistry in extracellular matrix research. Methods 45: 10 – 21 PubMed: 18442701
6) Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J. Histochem. Cytochem. 55: 853 – 866. PubMed: 17478447
7) Caterson B, Mahmoodian F, Sorrell JM, Hardingham TE, Bayliss MT, Carney SL, Ratcliffe A & Muir H (1990). Modulation of native chondroitin sulfate structure in tissue development and in disease. J. Cell Sci. 97: 411 – 417. PubMed: 1705939
8) Mehmet H, Scudder P, Tang, PW, Hounsell, EF, Caterson, B & Feizi T (1986). Antigenic determinants recognized by three monoclonal antibodies to keratan sulfate involve sulfated hepta- or larger oligosaccharides of the poly-N-acetyllactosamine series. Eur. J. Biochem. 157: 385 – 391. PubMed: 2423332
9) Funderburgh JL, Caterson B & Conrad GW (1986). Keratan sulfate proteoglycan during embryonic development of the chicken cornea. Developmental Biology 116: 267 – 277 PubMed: 2942429
10) Katz H, Austen KF, Caterson B, & Stevens RL (1986). Secretory granules of Heparin-containing Rat serosal mast cells also possess highly sulfated chondroitin sulfate proteoglycans. J. Biol. Chem. 261: 13393 - 13396 PubMed: 3531203 
11) Caterson B, Christner JE, Baker JR & Couchman JR (1985). The production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Federation Proceedings 44: 386 – 393. PubMed: 257841
12) Couchman JR, Caterson B, Christner JE & Baker JR (1984). Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature 307: 650 – 652. PubMed: 6420711
13) Caterson B, Christner JE & Baker JR (1983). Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan. J. Biol. Chem. 258: 8848 – 8854 PubMed: 6223038

Citations for Anti 6-Sulfated Unsaturated Disaccharide Neoepitopes (C-6-S "stubs") of Chondroitin Sulfate mAb (Clone 3B3) – 1 Found

Izumikawa, Tomomi; Dejima, Katsufumi; Watamoto, Yukiko; Nomura, Kazuko H; Kanaki, Nanako; Rikitake, Marika; Tou, Mai; Murata, Daisuke; Yanagita, Eri; Kano, Ai; Mitani, Shohei; Nomura, Kazuya; Kitagawa, Hiroshi. Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes. The Journal Of Biological Chemistry. 2016;291(44):23294-23304.