Anti Calbindin (D-28K) pAb (Rabbit, Affinity Purified)

Cosmo Bio LTD

Catalog No: CAC-ACC-PA003

Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.

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Background
Neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease have been increasing rapidly and have become a serious social problem. In recent years, new causative genes have been discovered for amyotrophic lateral sclerosis (ALS) and other intractable neurological diseases opening new avenues for research on pathogenesis. It has been suggested that aggregation and accumulation of specific proteins cause neurotoxicity and the formation of lesions, but the onset and progression mechanisms are still unclear. Neuropathological diagnostic and experimental model biomarkers are needed for drug construction, drug discovery, and therapeutic development.

Vitamin D-dependent 28 kDa calcium-binding protein (calbindin D 28k or CB) is a member of the calmodulin superfamily of calcium binding proteins discovered in 1966 by Wasserman and Taylor. The protein has a distinct distribution in the brain and sensory system and is abundant in specific neuronal cell types. Calbindin D-28k constitutes as much as 0.1-1.5 % of the total soluble protein in brain and it may be present at intracellular concentrations up to 1 mM. Several recent studies have shown that calbindin D can protect cells from degeneration and ischemic injury under extracellular stress. In particular, this is demonstrated in the brain of Alzheimer and quotes disease patients. Cells containing calbindin D have a considerably lower plaque and tangles burden than those lacking this 28 kDa protein.

Applications

Western Blotting (WB): 0.1 µg/ml
Immunofluorescence (IF) : 1 µg/ml
Immunoprecipitation (IP) : assay dependent
Enzyme-linked immunosorbent assay (ELISA) : assay dependent
Immunohistochemistry (Paraffin) (IHC (P)) : assay dependent 

Product description

Package Size	50 µg
Form	Liquid (0.1M NaPB, pH7.0, 20 mg/ml BSA, 0.1% Sodium Azide (NaN3) added)
Concentration	1mg/ml
Purity	Affinity Purified
Host	Rabbit Polyclonal
Immunogen	28 kDa calbindin-D protein purified from rat kidney
Specificity	Calbindin D-28k
Cross Reactivity	Bovine, Human, Mouse, Porcine, Rat
Subclass	IgG
Storage	Store below -20°C. Avoid freeze-thaw cycles.

References:
Zhu YY, Takashi M, Miyake K, Kato K. (1991) Sensitive enzyme immunoassay for human 28 kDa calbindin-D. Clin Chim Acta. 1991 201:183-92.