BrdU (5-Bromo-2-Deoxyuridine) is a derivative of uridine that can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA is a common method to quantify newly synthesized DNA and to identify cells in the S-phase of the cell cycle. BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells.
Monoclonal Antibody targets BrdU for FCM, ICC, IHC.
| Product Specifications | |
| Application | FC, ICC, IHC |
| Reactivity | Human |
| Clonality | Monoclonal IgG1 |
| Host | Mouse |
| Immunogen | 5-Iodouridine-Ovalbumin |
| Formulation | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Citations for Anti-Bromodeoxyuridine mAb (Clone: 2B1) – 26 Found |
| Nakajima, T; Yageta, M; Shiotsu, K; Morita, K; Suzuki, M; Tomooka, Y; Oda, K. Suppression of adenovirus E1A-induced apoptosis by mutated p53 is overcome by coexpression with Id proteins. Proceedings Of The National Academy Of Sciences Of The United States Of America. 1998;95(18):10590-5. PubMed - Comments: Used in: FCM |
| Liu, Q; VanHoy, R W; Zhou, J H; Dantzer, R; Freund, G G; Kelley, K W. Elevated cyclin E levels, inactive retinoblastoma protein, and suppression of the p27(KIP1) inhibitor characterize early development of promyeloid cells into macrophages. Molecular And Cellular Biology. 1999;19(9):6229-39. PubMed - Comments: Used in: FCM |
| Ito, Sayuri; Gotoh, Eisuke; Ozawa, Shigeru; Yanagi, Kazuo. Epstein-Barr virus nuclear antigen-1 is highly colocalized with interphase chromatin and its newly replicated regions in particular. The Journal Of General Virology. 2002;83(Pt 10):2377-2383. PubMed - Comments: Used in: ICC |
| Katou, Yuki; Kanoh, Yutaka; Bando, Masashige; Noguchi, Hideki; Tanaka, Hirokazu; Ashikari, Toshihiko; Sugimoto, Katsunori; Shirahige, Katsuhiko. S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature. 2003;424(6952):1078-83. PubMed - Comments: Used in: IP |
| Itakura, Eisuke; Sawada, Isao; Matsuura, Akira. Dimerization of the ATRIP protein through the coiled-coil motif and its implication to the maintenance of stalled replication forks. Molecular Biology Of The Cell. 2005;16(12):5551-62. PubMed - Comments: Used in: ICC |
| Imai, Fumiyasu; Hirai, Syu-ichi; Akimoto, Kazunori; Koyama, Hiromichi; Miyata, Takaki; Ogawa, Masaharu; Noguchi, Shigeru; Sasaoka, Toshikuni; Noda, Tetsuo; Ohno, Shigeo. Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. Development (Cambridge, England). 2006;133(9):1735-44. PubMed - Comments: Used in: IHC |
| Yeom, Seon-Yong; Kim, Geun Hyang; Kim, Chan Hee; Jung, Heun Don; Kim, So-Yeon; Park, Joong-Yeol; Pak, Youngmi Kim; Rhee, Dong-Kwon; Kuang, Shao-Qing; Xu, Jianming; Han, Duck Jong; Song, Dae-Kyu; Lee, Jae Woon; Lee, Ki-Up; Kim, Seung-Whan. Regulation of insulin secretion and beta-cell mass by activating signal cointegrator 2. Molecular And Cellular Biology. 2006;26(12):4553-63. PubMed - Comments: Used in: IHC |
| Hirai, Syu-ichi; Cui, De Feng; Miyata, Takaki; Ogawa, Masaharu; Kiyonari, Hiroshi; Suda, Yoko; Aizawa, Shinichi; Banba, Yumi; Ohno, Shigeo. The c-Jun N-terminal kinase activator dual leucine zipper kinase regulates axon growth and neuronal migration in the developing cerebral cortex. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience. 2006;26(46):11992-2002. PubMed - Comments: Used in: IHC |
| Yamashita, Naoya; Uchida, Yutaka; Ohshima, Toshio; Hirai, Syu-ichi; Nakamura, Fumio; Taniguchi, Masahiko; Mikoshiba, Katsuhiko; Honnorat, Jérôme; Kolattukudy, Pappachan; Thomasset, Nicole; Takei, Kohtaro; Takahashi, Takuya; Goshima, Yoshio. Collapsin response mediator protein 1 mediates reelin signaling in cortical neuronal migration. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience. 2006;26(51):13357-62. PubMed - Comments: Used in: IHC |
| Bermejo, Rodrigo; Doksani, Ylli; Capra, Thelma; Katou, Yuki-Mori; Tanaka, Hirokazu; Shirahige, Katsuhiko; Foiani, Marco. Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation. Genes & Development. 2007;21(15):1921-36. PubMed - Comments: Used in: IP |
| D'Ambrosio, Claudio; Schmidt, Christine Katrin; Katou, Yuki; Kelly, Gavin; Itoh, Takehiko; Shirahige, Katsuhiko; Uhlmann, Frank. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes & Development. 2008;22(16):2215-27. PubMed - Comments: Used in: IP |
| D'Ambrosio, Claudio; Schmidt, Christine Katrin; Katou, Yuki; Kelly, Gavin; Itoh, Takehiko; Shirahige, Katsuhiko; Uhlmann, Frank. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes & Development. 2008;22(16):2215-27. PubMed |
| Sugimura, Kazuto; Takebayashi, Shin-Ichiro; Taguchi, Hiroshi; Takeda, Shunichi; Okumura, Katsuzumi. PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA. The Journal Of Cell Biology. 2008;183(7):1203-12. PubMed - Comments: Used in: ICC |
| Tani, Hidenori; Mizutani, Rena; Salam, Kazi Abdus; Tano, Keiko; Ijiri, Kenichi; Wakamatsu, Ai; Isogai, Takao; Suzuki, Yutaka; Akimitsu, Nobuyoshi. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Research. 2012;22(5):947-56. PubMed - Comments: Used in: IP |
| Yamazaki, Satoshi; Ishii, Aii; Kanoh, Yutaka; Oda, Masako; Nishito, Yasumasa; Masai, Hisao. Rif1 regulates the replication timing domains on the human genome. The Embo Journal. 2012;31(18):3667-77. PubMed - Comments: Used in: IP |
| Yamazaki, Satoshi; Ishii, Aii; Kanoh, Yutaka; Oda, Masako; Nishito, Yasumasa; Masai, Hisao. Rif1 regulates the replication timing domains on the human genome. The Embo Journal. 2012;31(18):3667-77. PubMed - Comments: Used in: ICC |
| Tani, Hidenori; Imamachi, Naoto; Salam, Kazi Abdus; Mizutani, Rena; Ijiri, Kenichi; Irie, Takuma; Yada, Tetsushi; Suzuki, Yutaka; Akimitsu, Nobuyoshi. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. Rna Biology. 2012;9(11):1370-9. PubMed - Comments: Used in: IP |
| Alzu, Amaya; Bermejo, Rodrigo; Begnis, Martina; Lucca, Chiara; Piccini, Daniele; Carotenuto, Walter; Saponaro, Marco; Brambati, Alessandra; Cocito, Andrea; Foiani, Marco; Liberi, Giordano. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell. 2012;151(4):835-846. PubMed - Comments: Used in: IP |
| Tani, Hidenori; Torimura, Masaki; Akimitsu, Nobuyoshi. The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. Plos One. 8(1):e55684. PubMed - Comments: Used in: IP |
| Gonzalez-Huici, Victor; Szakal, Barnabas; Urulangodi, Madhusoodanan; Psakhye, Ivan; Castellucci, Federica; Menolfi, Demis; Rajakumara, Eerappa; Fumasoni, Marco; Bermejo, Rodrigo; Jentsch, Stefan; Branzei, Dana. DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. The Embo Journal. 2014;33(4):327-40. PubMed - Comments: Used in: IP |
| Chen, Qingfa; Arai, Daiki; Kawakami, Kazuki; Sawada, Takahiro; Jing, Xuefeng; Miyajima, Masayasu; Hirai, Syu-Ichi; Sakaguchi, Kazushige; Furushima, Kenryo. EphA4 Regulates the Balance between Self-Renewal and Differentiation of Radial Glial Cells and Intermediate Neuronal Precursors in Cooperation with FGF Signaling. Plos One. 10(5):e0126942. PubMed - Comments: Used in: IHC |
| Toteva, Tea; Mason, Bethany; Kanoh, Yutaka; Brøgger, Peter; Green, Daniel; Verhein-Hansen, Janne; Masai, Hisao; Thon, Geneviève. Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2017;114(5):1093-1098. PubMed - Comments: Used in: IP |
| Wang, Dan; Li, Juanjuan; Liu, Suning; Zhou, Hang; Zhang, Long; Shi, Wangpeng; Shen, Jie. spalt is functionally conserved in Locusta and Drosophila to promote wing growth. Scientific Reports. 2017;7:44393. PubMed - Comments: Used in: IHC |
| Gay, Sophie; Piccini, Daniele; Bruhn, Christopher; Ricciardi, Sara; Soffientini, Paolo; Carotenuto, Walter; Biffo, Stefano; Foiani, Marco. A Mad2-Mediated Translational Regulatory Mechanism Promoting S-Phase Cyclin Synthesis Controls Origin Firing and Survival to Replication Stress. Molecular Cell. 2018;70(4):628-638.e5. PubMed - Comments: Used in: IP |
| Yabe-Wada, Toshiki; Philpott, Caroline C; Onai, Nobuyuki. PCBP2 post-transcriptionally regulates sortilin expression by binding to a C-rich element in its 3' UTR. Febs Open Bio. 2020;10(3):407-413. PubMed - Comments: Used in: RIP |
| Chen, Qingfa; Song, Hao; Liu, Chuanguo; Xu, Jun; Wei, Chuanfei; Wang, Wei; Han, Fabin. The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo. Frontiers In Aging Neuroscience. 12:7. PubMed - Comments: Used in: IF |