Angiotensin-converting enzyme 2 (ACE2) protein

Cosmo Bio LTD

Catalog No: HAK-ACE2_UL-1

When the new coronavirus infects a cell, the spike protein on the surface of the virus binds to ACE2 on the cell surface. Drugs that block this binding will prevent viral infection.

1. ACE2 extracellular domain
2. SARS-CoV-2 spike protein RBD/rabbit Fc tag
3. Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag
4. Neutralizing antibodies

We have also started selling a monoclonal antibody ( anti-SARS-CoV-2 spike protein monoclonal antibody (mouse)) that is specific and has high affinity to the receptor binding domain of the spike protein S1 domain . This is a neutralizing antibody that inhibits the binding of the receptor binding domain to ACE2. 

Background

Angiotensin-converting enzyme 2 (ACE2), also known as ACE homolog, is an integral membrane protein that is highly homologous to ACE. ACE2 was known as one of the factors that work in the renin-angiotensin system, which is involved in blood pressure regulation. Recently, it has been revealed that when the novel coronavirus that causes COVID-19 infects human cells, it binds to ACE2 present in the cell membrane and is then taken into the cell, and ACE2 is also thought to be a receptor for the novel coronavirus. ⁽¹⁾ A highly sensitive binding assay is provided by immobilizing
human ACE2 protein with His tag (product number: HAK-ACE2_UL-1) on a 96-well plate and adding SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_UL-1) or biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_bio-1), which is a fusion protein in which the receptor binding domain of the viral protein is bound to rabbit Fc, and detecting with an HRP-labeled secondary antibody or streptavidin.

ACE2 extracellular domain

Product Information

Figure 1 SDS-PAGE

Protein Structure
Human ACE2 protein with His tag (HAK-ACE2_UL-1) contains the polyhistidine tagged C-terminus of Ser19-Pro738, which corresponds to the extracellular domain of human angiotensin-converting enzyme 2, expressed in HEK293 cells, and purified using a Ni column.
purity
>95% (SDS-PAGE)
composition
Human ACE2 protein, His tag, 1mg/mL, 0.1M PBS (pH 7.2-7.4)
keep
Avoid repeated freeze-thaw cycles. Upon receipt, store product at -70°C or colder. Aliquoting is recommended prior to use.
SDS-PAGE
430ng of this product was electrophoresed on a 4-20% gradient gel and stained with CBB (Figure 1).

Usage Example

1. Binding assay
• 0.156-10µg/mL of solid-phased human ACE2 protein/His tag (100µL/well) was bound to 0.8µg/mL of biotinylated SARS-CoV-2 spike protein RBD/rabbit Fc tag, and detected with HRP-labeled streptavidin. The
  linear range of the calibration curve was 0.156-1.25µg/mL (Figure 2).
2. Competitive binding inhibitor screening
• A binding inhibitor (SARS-CoV-spike protein RBD/rabbit Fc tag) was added at 10-5000ng/mL to the binding reaction of 1µg/mL solid-phased human ACE2 protein/His tag (100µL/well) and 600ng/mL biotinylated SARS-CoV-2 spike protein RBD/rabbit Fc tag, and the reaction was detected with HRP-labeled streptavidin. The 50% inhibitory concentration (IC50) was 140ng/mL (2.7nM) (Figure 3).