4x Ubiquitin-Rhodamine 110

Ubiquigent

Catalog No: UBI-60-0122-500

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Species Reactivity: human  

Source: synthetic  

Formula: DTT containing buffer  

Concentration: 0.4 µM  

Molecular Weight: ~8.93kDa  

Protein Sequence: Accession number: P62987. C-terminally tagged with Rhodamine 110  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: Activity Assay: The activity of Ubiquitin-Rhodamine 110 was validated by determining the increase in fluorescence at 535nm (Excitation 485nm) measured as a result of the enzyme catalysed cleavage at the amide bond between the C-terminal Glycine and Rhodamine, generating Ubiquitin and de-quenched Rhodamine 110-Glycine. UCHL3 (deubiquitylase) was incubated with Ubiquitin-Rhodamine 110 and the fluorescence was measured at four time points (0min, 30min, 60min and 90min).