Gene Cloning: ECOS™ Competent Cells

 

 

The world’s fastest and easiest to use chemically competent cells - 1 minute transformation!

With “ECOS™” standing for “E. Coli One-Step”, and its reverse “SOCE” standing for “SOC Eliminated”,  the name itself speaks for the advantages. 

1 minute, 1 step high-efficiency transformation and no SOC recovery step required, truly a design to save your time.

 

ECOS™ Competent Cells are the first innovative products of Yeastern Biotech’s R&D team, which strives to bring noble products that make research faster and easier. ECOS™ is Yeastern Biotech’s registered trademark in USA, Japan, Canada, Korea, and Taiwan. The name speaks for the merits of this line of products - E. Coli One-Step transformation. The reverse of this name is SOCE – SOC Eliminated. ECOS™ protocol allows users to finish the transformation within one step and to skip the SOC recovery step. Therefore, ECOS™ competent cells are the fastest transforming cells worldwide. The traditional protocol requires recovery step and takes 1 ~ 2 hours to finish, but ECOS™ allows transformation to be finished within 1 minute and the procedures are a lot simpler. Please check the ECOS™ protocol section. The latest progress in the ECOS™ product section is the development of another 3 protocols in addition to the standard ECOS™ protocol that was first introduced in 2003. The non-heat shock protocol allows transformation to be further simplified, and the 2 ~ 6-minute protocol enhances transformation efficiency significantly. These protocols have been developed based on the results of numerous experiments involving different antibiotics, plasmid size, and various conditions. Now, Yeastern Biotech offers our customers a new ECOS™ competent cells with similar transformation efficiency to that of electroporation for applications which require very high transformation efficiency such as the construction of genomic libraries.

 

Competent Cells Product Line-up
ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg)	ECOS X DH5α Competent Cells (>= 5 x 10^9cfu/μg)	ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg)	ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg)
Cat No.: YSN-FYE607	Cat No.: YSN-FYE610	Cat No.: YSN-FYE708	Cat No.: YSN-FYE709
ECOS 2163 GM2163 Competent Cells (>=5 x10^7 cfu/μg)	ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg)	ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)	ECOS SONIC Competent Cells (>=1x10^8cfu/μg)
Cat No.: YSN-FYE807	Cat No.: YSN-FYE508	Cat No.: YSN-FYE207	Cat No.: YSN-HYE007-Sonic

Features

• No LB/SOC required

• Revolutionary 1- or 6-minute transformation, instead of 1 ~ 2 hours

• Procedures are simplified

• Several protocols to choose from according to your needsin efficiency and convenience

• Strict QC process to check the efficiency of each batch produced

• Ideal for bench users to automatize cloning projects

• Electronically monitored shipping process to check temperature fluctuation during shipping

 

ECOS™ Protocol Comparison Table

 

 

 Transformation Protocol

ECOS™ 96-Well Protocol

Special Tips

Patented Technology

Yeastern Biotech Co., Ltd. owns the patent of ECOS™ technology (protocol) exclusively in USA, UK, Taiwan, and France (Patent No. US 6,864,088; GB 2383582; I 229696; FR 2832727, etc.). Under the protection of this patent, Yeastern Biotech is the only company that has the right to use the protocol with the claims that transformation with our ECOS™ chemically competent cells can be finished within one minute and SOC is not needed. If anyone intends to sell competent cells that use ECOS™ protocol or its similar version, please contact our headquarters in Taiwan for licensing.

Quality Control

Each lot of competent cells has to pass three quality control tests before shipping:

1. Efficiency test: each batch of ECOS™ should meet the claimed transformation efficiency at the time of production using Protocol 4 (Page 3-3) and supercoiled pUC19 DNA.
2. Contamination test: competent cells were plated directly on ampicillin plates without being transformed. To pass the test, no colonies should be seen after overnight incubation.
3. A-complementation test: this test is performed for all ECOS™ competent cells except for ECOS™ 21(DE3) and ECOS™ 2163. To pass the test, the ratio of white colonies over the total colonies should be less than 3%

Ordering Information

Competent Cells

Catalog Number	Product Name
YSN-FYE607	ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg)
YSN-FYE610	ECOS X DH5α Competent Cells (>= 5 x 10^9cfu/μg)
YSN-FYE708	ECOS 9-5 JM109 Competent Cells (>=1 x10^8cfu/μg)
YSN-FYE709	ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg)
YSN-FYE807	ECOS 2163 GM2163 Competent Cells (>=5 x10^7 cfu/μg)
YSN-FYE508	ECOS 10B DH10B Competent Cells (>=1x10^8 cfu/μg)
YSN-FYE207	ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)
YSN-HYE007-Sonic	ECOS SONIC Competent Cells (>=1x10^8 cfu/μg)