1) PCR Enzymes and Kits
Please note: Toyobo Products ARE Not available thru Cosmo Bio USA in China, Korea, Taiwan, Singapore, Thailand, Malaysia, Indonesia and Hong Kong.
Products on this page
- rTth DNA Polymerase DNA polymerase having reverse transcriptase activity
- rTaq DNA Polymerase Standard PCR Enzyme
- Quick Taq™ HS DyeMix Taq DNA polymerase (Dye-premix & Hot-start version)
- Blend Taq™ / Blend Taq™ -Plus- High Efficient Taq DNA polymerase
Toyobo has various unique PCR enzymes, kits and PCR related products as shown below. The following tables show the characteristics of each product. Detailed information can be obtained by going to the linked sites.
DNA polymerase having reverse transcriptase activity
rTth DNA Polymerase
rTth DNA Polymerase is a recombinant DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8. This polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions. Therefore, this enzyme enables "one-step RT-PCR" including reverse transcription and PCR steps.
Cat. No. TYB-TTH-301, 250 U, 100-200 reactions
| MORE DETAILS at Toyobo website | Ordering Information |
standard PCR enzyme
rTaq DNA Polymerase
rTaq DNA Polymerase is a recombinant DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq) YT-1.
Cat.No. TYB-TAP-201, 250 U, 100-200 reactions
| MORE DETAILS at Toyobo website | Ordering Information |
taq DNA polymerase (dye-premix & hot-start version)
Quick Taq™ HS DyeMix
Quick Taq™ HS DyeMix is a Taq-based 2 × master mix PCR reagent that contains an electrophoresis dye (BPB; bromophenol blue) and anti-Taq antibodies for hot start PCR. This reagent contains all components for PCR except primers and template DNA. This reagent shows specific and efficient amplification. The amplified products can be directly loaded in the wells of an agarose or acrylamide gel.
Cat. No TYB-DTM-101, 100 reactions
| MORE DETAILS at Toyobo website | Ordering Information |
high efficient taq DNA polymerase
Blend Taq™ / Blend Taq™ -Plus-
Blend Taq™ and Blend Taq™ -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method. This method uses a DNA polymerase which lacked 3'?5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a ‘mixed' enzyme solution enables highly efficient amplification. The enzyme solution of Blend Taq™ -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR. Blend Taq™ and Blend Taq™ -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
Cat. No. TYB-BTQ-101, 250 U, 200 reactions
Cat. No. TYB-BTQ-201, 250 U, 200 reactions
| MORE DETAILS at Toyobo website | Ordering info: Blend Taq™ | Ordering info: Blend Taq™ -Plus- }
PCR Enzyme & Kit
| Enzyme | Product Name | Fidelity (3'→5' Exonucle-aseactivity) | Efficiency | Velocity (Extension time) | Target size | Crude sample | Reverse Transcriptase Activity | Hot start | PCR Product Ends |
| Tth DNA polymerase | rTth DNA Polymerase | - [1] |
+ | + | ~2 kb | + | ✓ (Mn2+) |
- | 3'-dA |
| (1 min/kb) | |||||||||
| Taq DNA polymerase | rTaq DNA Polymerase | - [1] |
+ | + | ~2 kb | + | - | - | 3'-dA |
| (1 min/kb) | |||||||||
| Quick Taq™ HS DyeMix | - [1] |
+ | + | ~4 kb | + | - | ✓ | 3'-dA | |
| (1 min/kb) | |||||||||
| Blend Taq™ | + [3] |
++ | + | ~23 kb | ++ | - | - | mixed(blunt &3'-dA) | |
| (1 min/kb) | |||||||||
| Blend Taq™ -Plus- | + [3] |
++ | + (1 min/kb) |
~23 kb | ++ | - | ✓ |
[ ]: Accuracy of each PCR enzyme (reagent) when the accuracy of Taq DNA polymerase is set to 1.
PCR RELATED PRODUCTS
| Product Name | Remarks |
| Anti-Taq high | Anti-Taq DNA polymerase neutralization antibody for hot start |
| dNTPs Mixture(2mM) | Highly purified dNTPs for PCR |
| dNTPs Mixture(10mM) | |
| RNase Inhibitor | Highly purified RNase inhibitor for reverse transcription |
| TArget Clone™ | TA cloning of PCR products having overhanged dA at the 3'-ends. PCR products from Taq DNA polymerase, Blend Taq™ [Code No. BTQ-101], Blend Taq™ -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101] are available |
| TArget Clone™ -Plus- | TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201] or KOD FX [Code No. KFX-101]. |
| 10xA-attachment mix | Attachment of dA at the 3' - ends of blunt end PCR products from KOD -Plus- and KOD FX ( For TA cloning ) |
PCR RELATED PRODUCTS
Anti-Taq high Anti-Taq DNA polymerase antibody
This product is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. The product can be applied for hot start PCR using Taq DNA polymerase by mixing with the Taq DNA polymerase solution.
- Code No. TYB-TCP-101 100µL
dNTPs Mixture(2mM)
dNTPs Mixture(10mM)
- 2 mM and 10 mM solutions are available
- Suitable for PCR and reverse transcriptation
dNTPs Mixture is an equal moler solution mixture of ultrapure dATP, dCTP, dGTP and dTTP.
- Code No. TYB-NTP-201 2 µmoles/1 mL (2 mM)
- Code No. TYB-NTP-301 2 µmoles/0.2 mL (10 mM)
RNase inhibitor
This product is a recombinant type human ribonuclease inhibitor (RNase inhibitor; 51kDa) that is produced by E. coli. RNase inhibitor forms a complex with ribonuclease A (RNase A) and inhibits the activity of RNase A. This inhibitor can be applied to reverse transcriptase reactions.
- Code No. TYB-SIN-201 2,500 U
TArget Clone™ High efficient TA cloning kit
- Ligation step can be completed in 5 min with high efficiency.
- PCR products can be used without purification.
TArget Clone™ [Code No. TAK-101] can be applied to the TA cloning of PCR products amplified using Taq DNA polymerase, Blend Taq™ [Code No. BTQ-101], Blend Taq™ -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101]. Almost all PCR products having dA overhanging at the 3'end are available.
- Code No. TYB-TAK-101 10 reactions
TArget Clone™ -Plus- High efficient TA cloning kit for KOD products
- Can be applied to the TA cloning of blunt-end PCR products of KOD DNA polymerase.
- Ligation step can be completed in 5 min with high efficiency.
- PCR products can be used without purification.
TArget Clone™ -Plus- [Code No. TAK-201] can be applied to the TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201, KOD-401] or KOD FX [Code No. KFX-101, KFX-201]. TArget Clone™ -Plus- contains 10xA-attachment mix. This reagent is a mixture of anti-KOD DNA polymerase antibody specific to KOD 3'→5' exonuclease activity (proof-reading activity) and Taq DNA polymerase, which exhibits terminal transferase activity. The 10 x A-attachment mix allows for blunt end PCR products to acquire overhanging dA at the 3'-ends
- Code No. TYB-TAK-201 10 reactions
10xA-attachment mix dA attachment reagent
- The attachment reaction is completed in 10 minutes at 60ºC.
- The cloning efficiency of dA attached PCR products is as great as that of PCR products of Taq DNA polymerase.
10 x A-attachment mix is a reagent consists of the anti-KOD DNA polymerase antibody for the 3'→5' exonuclease activity (proof-reading activity) of the enzyme, and Taq DNA polymerase exhibits the terminal transferase activity. The PCR products from KOD -Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] have blunt ends due to the 3'→5' exonuclease activity of KOD DNA polymerase. 10 x A-attachment mix allows the PCR products to acquire overhanging dA at the 3'-ends. The products having 3'-dA overhang can be directly cloned into arbitrary T-vectors using ligation reagents such as Ligation high Ver.2 [Code No. LGK-201].
- Code No. TYB-TAK-301 25 reactions