Spin column-based Antibody Purification Kit 

Protein A  / Protein G

 

Background

This spin-column type IgG antibody-purify kit consists of immobilized Protein A (Catalog Number: CSR-APK-10A) or Protein G (Catalog Number: CSR-APK-10G) on the solid phase of filter type monolithic silica with uniform continuous pores. Compared with conventional ones, this product can easily purify IgG antibodies in a shorter time (approximately 3 minutes). The column can be recycled up to 5 times without diminishing antibody binding capacity. Each can purify IgG antibodies up to 0.4 mg with Protein A and 0.3 mg with Protein G per column under more than 90% of recovery rate. 1: In case purified antibodies or culture supernatants are used, up to five replicates of the columns can be reused. However, if the sample contains a solid component such as protein in the serum, it may cause a clogging of the column, which may result in a decrease in the regeneration frequency and fluid permeability. Therefore, this product should be used after removal of the solid component by centrifugal removal or filter filtration.

Composition

Kit contains sufficient reagents for 60 individual purifications (approx. 3 runs per column)

Component	Quantity
Monolithic silica-based Spin Column	10 Columns
Sampling tube	10 tubes
Binding Buffer	30 ml
Wash Buffer	30 ml
Elution Buffer	30 ml
Neutralization Buffer	15 ml
Regeneration Buffer	30 ml

Catalog Number / Product Name	Column Type	Recommended Sample
CSR-APK-10G	Protein G	Monoclonal Antibody	Human IgG1, IgG2, IgG3, IgG4
Spin column-based Antibody Purification Kit  (Protein G)			Mouse IgG1, IgG2a, IgG2b, IgG3
		Polyclonal Antibody	Rabbit, Goat
CSR-APK-10A	Protein A	Monoclonal Antibody	Human IgG1, IgG2,  IgG4
Spin column-based Antibody Purification Kit  (Protein A)			Mouse IgG2a, IgG2b, IgG3
		Polyclonal Antibody	Rabbit

Procedure and required solution amount

Features

• Easy-to-handle spin columns
• Fast purification process: IgG antibodies can be purified in aprox. 3 minutes!
• Column can be re-used (max. 5 times)

References

Hattori,et al. A small molecule compound with an indole moiety inhibits the main protease of SARS-CoV-2 and blocks virus replication. (2021) Nat Commun.28:12:668.

Oshiro,et al. Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2. (2021) J Virol Methods. 294 : 114183.

Protein G
Takamatsu,et al. SARS-CoV-2-neutralizing humoral IgA response occurs earlier but modest and diminishes faster compared to IgG response. (2022) bioRxiv. Jun 9;2022.06.09.495422.

Protein A
Maegawa,et al. Anti-calcitonin gene-related peptide antibody attenuates orofacial mechanical and heat hypersensitivities induced by infraorbital nerve injury. (2021) Biochem Biophys Res Commun.10 ; 569 : 147-153.