Cosmo Bio Brand Reagents for PORCINE Reproductive Engineering

Defined system for in vitro production of porcine embryos using a single basic medium

Offered here are the IVF reagents developed by groups in Japan over more than 10 years and commercialized by the IFP (Institute for Functional Peptides. Cosmo Bio sells and supports IFP's products under our CSR reagent brand program.

Porcine IVF Product Name (scroll down for product descriptions)	Cosmo Bio USA Cat. No.	IFP Cat. No.	Size
Basic Culture Kit for Porcine IVF Embryo Production	CSR-CK029	IFP100PEP-BK	1 kit
Complete Culture Kit Porcine IVF Embryo Production	CSR-CK030	IFP101PEP-CK	1 kit
POE-CM (Porcine Oocyte/Embryo Collection Medium)	CSR-CK020	IFP1040P	5 x 100 ml
POM (Basic Medium For Porcine Oocyte Maturation)	CSR-CK021	IFP1010P	3 x 25 ml
HP-POM (High Performance Porcine Oocyte Maturation Medium)	CSR-CK022	IFP1011P	3 x 25 ml
dbcAMP-100X (dbcAMP Concentrate, 100x Solution)	CSR-CK027	IFP1060P	500 ul
SEM-5X (Porcine Semen Dilution Medium)	CSR-CK026	IFP1050P	25 ml
PFM (Porcine Fertilization Medium): CSR-CK023	CSR-CK023	IFP1020	2 x 100 ml
PZM-5 (Porcine Embryo Development Medium)	CSR-CK024	IFP0410	3 x 25 ml
PBM (Porcine Blastocyst Medium)	CSR-CK025	IFP1030	2 x 25 ml
Reproplate - collagen free 6-well plate for IVF procedures	CSR-CK028		10 x 10 plates

 

Brief bibliography for defined porcine IVF reagents

• In Vitro Culture of Late Stage Pig Embryos in a Chemically Defined Medium, Porcine Blastocyst Medium (PBM).
  Mito T, Hoshi H., Methods Mol Biol. 2019;2006:105-113.
  PMID: 31230275
• Birth of piglets from in vitro-produced porcine blastocysts vitrified and warmed in a chemically defined medium.
  Mito T, Yoshioka K, Noguchi M, Yamashita S, Misumi K, Hoshi T, Hoshi H. Theriogenology. 2015 Nov;84(8):1314-20.
  PMID: 26255223
  
• Recombinant human follicle-stimulating hormones and transforming growth factor-alpha enhance in vitro maturation of porcine oocytes. 
  Mito T, Yoshioka K, Noguchi M, Yamashita S, Hoshi H.  Mol Reprod Dev 2013;80:549-560. 
  PMID: 23661505
  
• Glucose and glycine synergistically enhance the in vitro development of porcine blastocysts in a chemically defined medium.
  Mito T, Yoshioka K, Yamashita S, Suzuki C, Noguchi M, Hoshi H.   Reprod Fertil Dev. 2012;24(3):443-50.
  PMID: 22401276
  
• Transforming growth factor-α in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure. 
  Mito T, Yoshioka K, Nagano M, Suzuki C, Yamashita S, Hoshi H.  Theriogenology 2009; 72: 841-850.
  PMID: 19632712
  
• Defined system for in vitro production of porcine embryos using a single basic medium. 
  Yoshioka K, Suzuki C, Onishi A.  J Reprod Dev 2008; 54: 208-213. 
  PMID: 18408352 
• Glutamine and hypotaurine improves intracellular oxidative status and in vitro development of porcine preimplantation embryos. 
  Suzuki C, Yoshioka K, Sakatani M, Takahashi M.  Zygote 2007; 15: 317-324.
  PMID: 17967211
  Development to the blastocyst stage, the oxidative state, and the quality of early developmental stage of porcine embryos cultured in alteration of glucose concentrations in vitro under different oxygen tensions.
• Karja NW1, Kikuchi K, Fahrudin M, Ozawa M, Somfai T, Ohnuma K, Noguchi J, Kaneko H, Nagai T. Reprod Biol Endocrinol. 2006 Nov 6;4:54
  PMID: 17087833
• Effects of amino acid supplements and replacement of polyvinyl alcohol with bovine serum albumin in porcine zygote medium. 
  Suzuki C, Yoshioka K.  Reprod Fertil Dev 2006; 18: 789-795.
  PMID: 17032588
  
• Birth of piglets through the non-surgical transfer of blastocysts produced in vitro. J Reprod Dev. 
  Suzuki C1, Iwamura S, Yoshioka K.  2004 Aug;50(4):487-91.
  PMID: 15329482
  
• Production of piglets derived from in vitro-produced blastocysts fertilized and cultured in chemically defined media: effects of theophylline, adenosine, and cysteine during in vitro fertilization. 
  Yoshioka K, Suzuki C, Itoh S, Kikuchi K, Iwamura S, Rodriguez-Martinez H.  Biol Reprod 2003; 69: 2092-2099. 
  PMID: 12930720 
• Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. 
  Yoshioka K, Suzuki C, Tanaka A, Anas IMK, Iwamura S.  Biol Reprod 2002; 60: 112-119.
  PMID: 11751272
  
• Long-term preservation test of swine liquid semen using various diluted stock solutions. 
  Masaru Sone, Mikio Chihisa, Mitsutoshi Yoshida, Kimio Banba, Jun Ogasa.  The Japan Pig Association 1992; 29: 41-50. (in Japanese only)

Kits

Basic culture kit for in vitro fertilized egg production. Cat. No. CSR-CK029, 1 kit (IFP100PEP-BK)
Porcine in vitro fertilized egg production consisting of egg / embryo recovery solution (POE-CM), basic medium for egg maturation (POM), sperm fluid (PFM), culture medium for embryo development (PZM-5), dbcAMP concentrate, and reproplate Basic culture set. Under low oxygen culture conditions (5% O₂ /5% CO₂ /90% N₂ , 39° C), transplantable embryos (blastocysts) can be produced efficiently without co-culture of cumulus / granular membrane cells.

POE-CM (100 mL x 3)
POM (25 mL x 2)
PFM (100 mL x 1)
PZM-5 (25 mL x 1)
dbcAMP (100 times solution) (0.3 mL x 1)
Repro Plate (10 sheets ) × 3 pack)

 

Complete Culture Kit fo in vitro Fertilized Egg Production. Cat. No. CSR-CK030, 1 kit (IFP101PEP-CK)
A culture kit for the efficient production of high-quality embryos (blastocysts)by adding porcine late embryo culture medium (PBM) to basic culture set for in vitro fertilized egg production. This medium kit is particularly effective for the production of high-quality embryos, which is effective in increasing the number of blastocyst cells and forming escaped blastocysts.

POE-CM (100mL x 3)
POM (25mL x 2)
PFM (100mL x 1)
PZM-5 (25mL x 1)
PBM (25mL x 1)
dbcAMP (100 times solution) (0.3mL x 1) Book)
Repro plate (10 sheets x 3 packs)

  

Individual Reagents

POE-CM (porcine oocyte/embryo collection medium: CSR-CK020, 5x100ml (IFP1040P)
A collection solution suitable for collecting and washing pig eggs and embryos. Yoshioka et al. 1) PXM-Hepes medium is a completely synthetic medium with phenol red added. Ingredients include inorganic salts, sugars, polyvinyl alcohol (PVA), Hepes, baking soda and phenol red. It is sterilized and can be used as it is. Stable pH even when used at 37 ° C and atmospheric pressure. 1) Yoshioka.K., Et al J. Reprod. Dev., 54: 208-213, 2008.

POM (basic medium for porcine egg ripening): CSR-CK021, 3x25ml (IFP1010P)

Yoshioka et al. 1) in the reported porcine oocytes maturation medium (modified medium supplemented with polyvinyl alcohol (PVA) in POM), fully synthetic base medium suitable for egg maturation. This medium is sterilized and can be used as is. This medium effectively promotes egg maturation by low oxygen culture (5% O 2 /5% CO 2 /90% N 2 , 39 ° C).

HP-POM (Basic medium for maturation of high-performance porcine ova): CSR-CK022,3x25ml (IFP1011P)
Serum-free basal medium optimized for egg maturation, a high-performance medium supplemented with TGF-α and other basic medium for porcine ovum maturation Using this medium, the oocyte maturation rate is almost the same as that of the POM medium, but it has been reported that the embryo development rate (blastocyst development rate) after in vitro fertilization is high (Mito et al.) 1) . This medium is sterilized and can be used as is. The use of this medium effectively promotes egg maturation by performing low oxygen culture (5% O 2 /5% CO 2 /90% N 2 , 39 ° C).


dbcAMP-100X (dbcAMP concentrate (100-fold solution)) : CSR-CK027, 500ul (IFP1060P)
It is effective for normal maturation of porcine ovum by adding to basal medium for porcine ovum maturation (POM) or basic medium for maturation of porcine ovum (HP-POM) used for ovum maturation culture. This concentrate is sterilized and can be used by adding it directly to the maturation medium.

IVF

SEM-5X (semen dilution for medium): CSR-CK026, 25ml (IFP1050P)
Can be used to prepare isotonic percoll solution for washing and adjusting semen It is sterilized and can be used as it is.

 
PFM (porcine semen): CSR-CK023 (IFP1020)
Completely synthetic medium based on PGMtac4 medium (Yoshioka et al.) 1) , a medium optimized for porcine in vitro fertilization . Ingredients contained in the medium include inorganic salts, sugars, baking soda, theophylline, adenosine, and polyvinyl alcohol. This medium is sterilized and can be used as is. This medium effectively promotes in vitro fertilization by performing hypoxic culture (5% O 2 /5% CO 2 /90% N 2 , 39 ° C). 1) Yoshioka. K., et al., J. Reprod. Dev., 54: 208-213, 2008. Embryo Development

PZM-5 (Porcine embryo development medium): CSR-CK024, 3x25ml (IFP0410)
The product, Yoshioka et al., 1,2) porcine IVF embryo development medium developed by (PZM-4) completely synthetic medium with improved 3) is. This medium can also be used to culture in vitro fertilized embryos or porcine in vivo fertilized embryos collected after in vivo fertilization. This medium contains the components necessary for culturing naked fertilized embryos, so it can be used without preparing a special medium. By performing hypoxic culture (5% O 2 /5% CO 2 /90% N 2 , 39 ° C), embryo production (blastocyst) can be produced efficiently. This medium is sterilized and can be used immediately.
1) Yoshioka. K., et al., Biol. Reprod., 60: 112-119, 2002. 
2) Yoshioka. K., et al., Biol. Reprod., 69: 2092-2099, 2003. 
3) Yoshioka.K., Et al., J. Reprod. Dev., 54: 208-213, 2008. 
 

PBM：CSR-CK025, 2x25ml (IFP1030)
PBM medium is prepared by adding glucose and glycine to porcine embryo development medium (PZM-5). After in vitro fertilization (measles), culture in PZM-5 medium first, and then change to PBM medium after late development of morula, improving blastocyst incidence, increasing blastocyst cell number, escape A medium that is effective in increasing the rate of blastocyst formation. The optimal culture conditions when using this medium are low oxygen cultures (5% O 2 /5% CO 2 /90% N 2 , 39 ° C). This medium is sterilized and can be used immediately.
 

 
Reproplate (collagen-free 6-well plate): CSR-CK028, 10x10 plates (IFP9670)
It is a culture plate that has the best shape for ovum maturation culture, in vitro fertilization, and fertilized egg embryo culture (mortar-shaped 6-hole), and provides high egg maturation rate, in vitro fertilization rate, and embryo development rate. As a feature of the repro plate, You can save culture fluid, semen and mineral oil. The shape is optimal for observation and culture operations under a stereo microscope. It is also ideal for in vitro maturation, in vitro fertilization, and fertilized egg culture of a small number of eggs. It can also be used to remove cryoprotectants in the vitrification preservation method and indirect freezing method.

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TALP-HEPES solution: Made to Order IFP0411P

This product is partially modified in composition of TALP solution developed by Bavister et al. TALP-Hepes solution can be used as a washing and recovery solution for pig fertilized embryos and eggs.

This product is made to order.
Please contact us for details.

 

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Porcine embryo vitrification preservation solution kit (PEV-SK): IFP16PVSK

A vitrification solution kit for ultra-low temperature preservation of pig embryos. A set of primary equilibration liquid, secondary equilibration liquid, vitrification liquid, and heating / dilution liquid. All product specifications consist of known ingredients.

"Porcine embryo vitrification preservation kit instruction manual" (PDF file is displayed)

 

Development to the blastocyst stage, the oxidative state, and the quality of early developmental stage of porcine embryos cultured in alteration of glucose concentrations in vitro under different oxygen tensions.Karja NW1, Kikuchi K, Fahrudin M, Ozawa M, Somfai T, Ohnuma K, Noguchi J, Kaneko H, Nagai T.Reprod Biol Endocrinol. 2006 Nov 6;4:54PMID: https://www.ncbi.nlm.nih.gov/pubmed/?term=17087833