About Cosmo Bio USA Suppliers

Serumwerk

	Density Gradient Media Excellence in Separations   Lymphoprep Lymphoprep Tube OptiPrep Polymorphprep Nycodenz Polysucrose 400
Supplier Name: Serumwerk Bernburg AG Supplier Location: Bernburg, Germany Founding Date: 1954 Distributed by Cosmo Bio USA Since: 2010 Supplier Website: https://diagnostic.serumwerk.com CBU Supplier Code (cat. no. prefix): AXS-

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Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macro-molecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology.

The most commonly used method of separating suspensions of mixed populations of biological particles is centrifugation in density gradients - and the choice of medium used to form the gradients is critical for the retention of particles function. Since the early sixties Nycomed have been developing and producing iodinated compounds as X-ray imaging agents. It was recognized early on that these compounds were also well suited to the formation of density gradients and offered new opportunities for improved methods of separating biological particles, especially osmotically-sensitive cells and subcellular organelles. Nycomed has therefore been actively involved over the last 30 years in the research and development of density gradient media.

Product	Description	Product Documents
OptiPrep™ (iodixanol solution) Iodixanol: 60% (w/v)  Density: 1.320 ± 0.001 g/ml  Osmolality: approx. 170 mOsm  Endotoxins: < 1.0 EU/ml	The Optimal Density Gradient Media OptiPrep is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance.	OptiPrep™ Leaflet OptiPrep™ Package Insert OptiPrep™ MSDS Optiprep™ rAAV references
Nycodenz™ (iohexol powder)	A Universal Density Gradient Media Nycodenz® is an off-white powder, freely soluble in water. Solutions up to 80% (v/w) with a density of 1.426 g/ml can be prepared.	Nycodenz™ Leaflet Nycodenz™ Package Insert Nycodenz™ MSDS
Lymphoprep™ Sodium diatrizoate: 9.1% (w/v) Polysaccharide: 5.7% (w/v) Density: 1.077 ± 0.001 g/mL Osmolality: 280 ± 15 mOsm Endotoxins:	Ready-Made Solution for Isolating Human Mononuclear Cells (PBMC). GMP compliant and ISO 13485 certified. Lymphohoprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human mononuclear cells.	Lymphoprep™ Leaflet Lymphoprep™ Package Insert Lymphoprep™ MSDS
Lymphoprep Tube™	Convenient Fritted Centrifuge Tubes Prefilled with Lymphoprep™.  GMP compliant and ISO 13485 certified. Lymphoprep™ Tube is a sterile tube in which the Lymphoprep™ is contained below a plastic filter disc. This allows diluted blood to be poured simply and directly into the tube, the disc preventing any mixing with the separation medium.	Lymphoprep™ Tube Leaflet Lymphoprep™ Tube Package Insert Lymphoprep™ Tube MSDS	Diatrizoic Acid (aka Hypaque™) - the major component of Lymphoprep™. Hypaque is a trademark of GE Healthcare.
Polymorphprep™ Sodium diatrizoate: 13.8% (w/v). Dextran 500: 8.0% (w/v). Density: 1.113 ± 0.001 g/ml. Osmolality: 440-500  mOsm/kg. Endotoxins: < 1.0 EU/ml.	Ready-Made Solution For Isolation of Human Polymorphonuclear Cells Polymorphprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human polymorphonuclear leucocytes (granulocytes).	Polymorphprep™ Leaflet Polymorphprep™ Package Insert Polymorphprep™ MSDS Polymorphprep™ Application Sheet
Polysucrose™ 400	Versatile Non-Ionic, Hydrophillic, Water-Soluble Powder for Density Gradient Separations And Other Applications Polysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. The product is similar to Ficoll® 400 from GE Healthcare.	Polysucrose™ 400 Leaflet Polysucrose™ 400 MSDS