Chondroitin Sulfate Proteoglycan Antibodies

Molecules involved in the formation of neural circuits and neurotransmission

Neuroglycan is a type of chondroitin sulfate proteoglycan (CSPG) that is expressed specifically in the brain and is known to be a molecule involved in the formation of neural circuits and neurotransmission. Neuron-glial Antigen 2 (NG2), also known as CSPG4, is a neuroglycan that regulates cell-substrate interactions during melanoma cell differentiation and migration at the endothelial basement membrane, while inhibiting neurite outgrowth and growth cone collapse during axon regeneration It has also been suggested that the molecule is an inhibitor of neurite outgrowth and growth cone collapse during axonal regeneration.

Data

Figure1: Immunostainings of a malignant glioblastoma (Cat.#PRPG-NG-M01, Clone 2161D7)

A) and cutaneous melanoma C) lesion. B) Western blotting on whole tissue/cell lysates of human glioblastoma lesion and the human melanoma A375 cell line after SDS-PAGE under reducing conditions on 4-10% gradient gels. D) immunostaining of human melanoma MeWo cells. Nuclear counterstasting was performed with Hoescht.

Figure2: Immunoblottings and Immunostainings (Cat.#PRPG-VS-M02, Clone 4C5)

A)	Immunoblotting of intact versican (mixture of V1 and V2 isoforms) prior to (Intact) and after combined chondroitinase ABC (Chase ABC) and endo- -galactosidase-digestion (Digested) form. The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard). Banding pattern depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, isoforms V0, V1 and V2 do not enter conventional polyacrylamide gels and therefore alternative gel types are strongly recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.
B)	Immunocytochemistry on cultured smooth muscle cells showing versican distribution in the ECM deposited by the cells.
C)	Immunostaining of versican in normal human skin
D)	Immunostaining of versican distribution in the Bowman capsule of a normal human kidney (PFA-fixed frozen section).

Figure3: Immunoblottings and Immunostainings (Cat.#PRPG-AG-M03, Clone 5G2)

A)	Immunoblotting of a human articular cartilage aggrecan preparation following SDS-PAGE on 3-8% linear gradient gels under reducing conditions, prior to (Intact) and after combined chondroitinase ABC and keratanase II digestion (Digested)
B)	Immunostaining for aggrecan in human articular cartilage.
C)	Immunostaining for aggrecan in human adult cerebral cortex (arrows point to neurons and perineuronal nets)

Product List

Chondroitin Sulfate Proteoglycan Antibodies

Product name	Catalog Number
Anti NG2 / CSPG4 (2161D7), Human (Mouse)	PRPG-NG-M01
Anti Versican / CSPG2 (4C5), Human (Mouse)	PRPG-VS-M02
Anti Aggrecan (5G2), Human (Mouse)	PRPG-AG-M03
Anti Aggrecan (7B7), Human (Mouse)	PRPG-AG-M04