Mebiol (MBG) Literature Citations and References
Mebiol® Gel Literature Citations and References
Cell Culture and Tissue Regeneration
Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation)
A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation.
Lei Y, Schaffer DV.
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):E5039-48. doi: 10.1073/pnas.1309408110. Epub 2013 Nov 18.PMID: 24248365
Stem Cells (corneal limbal), review
Towards the use of hydrogels in the treatment of limbal stem cell deficiency
Bernice Wright, Shengli Mi, Che J. Connon
Drug Discovery Today, Volume 18, Issues 1–2, January 2013, Pages 79-86 a
PMID: 22846850
Cell Culture (kidney cyst formation)
Mxi1 influences cyst formation in three-dimensional cell culture.
YJ Yook, KH Yoo, SA Song, MJ Seo, JY Ko, BH Kim, EJ Lee, E Chang, YM Woo, and JH Park
BMB Rep, Mar 2012; 45(3): 189-93.
PMID: 22449707
ROS Cell-Based Assay
Determination of Chronic Inflammatory States in Cancer Patients Using Assay of Reactive Oxygen Species Production by Neutrophils
Yoko Suzuki, Satoshi Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, And Katsuhiko Suzuki
Anticancer Res, Feb 2012; 32: 565 - 570.
PMID: 22287746
ROS Cell-Based Assay
Effect of Green Tea Extract on Reactive Oxygen Species Produced by Neutrophils from Cancer Patients
Katsuhiko Suzuki, Satoshi Ohno, Yoko Suzuki, Yumiko Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Ken-O Ishihara, Tsutomu Nozaki, Shigeki Miura, Hiroshi Yoshioka, And Yuichi Mori
Anticancer Res, Jun 2012; 32: 2369 - 2375.
PMID- 22641677
Stem Cell Culture, Regenerative Medicine
Application of a Thermo-Reversible Gelation Polymer, Mebiol Gel, for Stem Cell Culture and Regenerative Medicine
Kataoka K and Huh N*
2010 Vol. 6 (1): p10-14 (2010)
full text online
http://www.pubstemcell.com/monthly/006010200003.htm
Organ Culture
FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation.
Hiramatsu R, Harikae K, Tsunekawa N, Kurohmaru M, Matsuo I, Kanai Y.
Development. 2010 Jan;137(2):303-12. doi: 10.1242/dev.040519.
PMID: 20040496
Stem Cells (corneal limbal)
Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model.
G Sitalakshmi, B Sudha, HN Madhavan, S Vinay, S Krishnakumar, Y Mori, H Yoshioka, and S Abraham
Tissue Eng Part A, Feb 2009; 15(2): 407-15.
PMID: 18724830
Stem Cell (corneal limbal)
Limbal Stem Cells: Application in Ocular Biomedicine Review Article
Geeta K. Vemuganti, Anees Fatima, Soundarya Lakshmi Madhira, Surendra Basti, Virender S. Sangwan
International Review of Cell and Molecular Biology, Volume 275, 2009, Pages 133-181
PMID: 19491055
Virus infection/replication system
3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV
Hussein Hassan Aly, Kunitada Shimotohno, Makoto Hijikata
Biochemical and Biophysical Research Communications, Volume 379, Issue 2, 6 February 2009, Pages 330-334
PMID: 19103167
Embryo Explant Culture
Antagonism between Smad1 and Smad2 signaling determines the site of distal visceral endoderm formation in the mouse embryo.
Yamamoto M, Beppu H, Takaoka K, Meno C, Li E, Miyazono K, Hamada H.
J Cell Biol. 2009 Jan 26;184(2):323-34. doi: 10.1083/jcb.200808044. Epub 2009 Jan 19.
PMID:19153222
Hepatocyte Transplantation
Intraperitoneal Transplantation Of Hepatocytes Embedded In Thermoreversible Gelation Polymer (Mebiol Gel) In Acute Liver Failure Rat Model
N. Parveen, A.A. Khan, S. Baskar, M.A. Habeeb, P. Ravindra Babu, A. Samuel, Y. Hiroshi, M. Yuichi, C.M. Habibullah
Journal of Hepatology, Volume 48, Supplement 2, 2008, Page S71
http://hepatmon.com/?page=article&article_id=529
Stem Cells (mesenchymal)
Chrondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in chemicially synthesized thermoreversible polymer.
Kao, I, et al.
Chinese J. Physiology, 51(4), 252-258 (2008)
Hepatocyte Culture
Serum-derived hepatitis C virus infectivity in interferon regulatory factor-7-suppressed human primary hepatocytes
Hussein H. Aly, Koichi Watashi, Makoto Hijikata, Hiroyasu Kaneko, Yasutugu Takada, Hiroto Egawa, Shinji Uemoto, Kunitada Shimotohno
Journal of Hepatology, Volume 46, Issue 1, January 2007, Pages 26-36
PMID: 17112629
Stem Cell (Corneal Limbal)
Cultivation of human corneal limbal stem cells in Mebiol gel--A thermo-reversible gelation polymer.
B Sudha, HN Madhavan, G Sitalakshmi, J Malathi, S Krishnakumar, Y Mori, H Yoshioka, and S Abraham
Indian J Med Res, Dec 2006; 124(6): 655-64.
PMID: 17287553
Tissue Engineering (Bone)
In vitro culture of chondrocytes in a novel thermoreversible gelation polymer scaffold containing growth factors.
Yasuda A, Kojima K, Tinsley KW, Yoshioka H, Mori Y, Vacanti CA.
Tissue Eng. 2006 May;12(5):1237-45.
PMID: 16771637
Stem Cells (epithelial)
Isolation of epithelial stem cells from dermis by a three-dimensional culture system.
Medina RJ, Kataoka K, Takaishi M, Miyazaki M, Huh NH.
J Cell Biochem. 2006 May 1;98(1):174-84.
PMID: 16408300
Stem Cells (Corneal Limbal)
Comparative Study on Growth Characteristics of Cadaveric Human Corneal Limbal Stem Cells in Mebiol Gel (a Synthetic Polymer) and on Human Amniotic Membrane. H.N. Madhavan, B. Sudha1, G. Sitalakshmi, S. KrishnaKumar, Y. Mori, H. Yoshioka and S. Abraham.
Invest Ophthalmol Vis Sci 2006;47: E-Abstract 3033. 3033—B186
Tissue Regeneration (Liver)
Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver.
Nagaya M, Kubota S, Suzuki N, Akashi K, Mitaka T.
Hepatology. 2006 May;43(5):1053-62.
PMID: 16628635
Virus Reproduction and Drug Screening
Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b.
Murakami K, Ishii K, Ishihara Y, Yoshizaki S, Tanaka K, Gotoh Y, Aizaki H, Kohara M, Yoshioka H, Mori Y, Manabe N, Shoji I, Sata T, Bartenschlager R, Matsuura Y, Miyamura T, Suzuki T.
Virology. 2006 Aug 1;351(2):381-92. Epub 2006 May 6.
PMID: 16678876
Embryo Culture
Canonical Wnt Signaling and Its Antagonist Regulate Anterior-Posterior Axis Polarization by Guiding Cell Migration in Mouse Visceral Endoderm.
Chiharu Kimura-Yoshida, Hiroshi Nakano, Daiji Okamura, Kazuki Nakao, Shigenobu Yonemura, Jose A. Belo, Shinichi Aizawa, Yasuhisa Matsui, Isao Matsuo
Developmental Cell, Volume 9, Issue 5, November 2005, Pages 639-650
PMID: 16256739
Evalulation of Cell Line Growth in Mebiol Gel
H.N. Madhavan, J. Malathi, Patricia Rinku Joseph, Mori Yuichi, Samuel JK Abraham and Hiroshi Yoshioka. A study on the growth of continuous culture cell lines embedded in Mebiol Gel., Current Science, 87(9), 1275~77(2004). pdf
Stem Cell Culture and Differentiation
Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer.
Hishikawa K, Miura S, Marumo T, Yoshioka H, Mori Y, Takato T, Fujita T.
Biochem Biophys Res Commun. 2004 May 14;317(4):1103-7.
PMID: 15094382
Liver Regeneration
Evaluation of Thermoreversible gelation polymer for Regeneration of Focal Liver Injury.
M. Nagaya, S. Kubota, N. Suzuki, M. Tadakoro, K. Akashi.
Eur Surg Res, 36:95-103 (2004).
PMID: 15007262
Spheroid Culture (Cancer)
S. Tsukikawa, H. Matsuoka, Y. Kurahashi, Y. Konno, K. Satoh, R. Satoh, A. Isogai, K. Kimura, Y. Watanabe, S. Nakano, J. Hayashi, and S. Kubota.
A new method to prepare multicellular spheroids in cancer cell lines using a thermo-reversible gelation polymer, Artifcial Organs, 27(7), 598 -604(2003).
PMID: 12823414
Wound healing
Wound Dressing of Newly Developed Thermo gelling Thermo reversible Hydro gel.
H. Yoshioka, Y. Mori, S. Kubota ,
Jpn J Artif Organs, 27(2), 503 -506 (1998).
(Japanese Publication- Abstract in English) pdf
Islet Transplantation
In Vitro Studies on a New Method for Islet Micro encapsulation Using a Thermo reversible Gelation Polymer, N-Isopropylacrylamide-Based Copolymer.
S. Shimizu, M. Yamazaki, S. Kubota, T. Ozasa, H. Moriya, K. Kobayashi, M. Mikami, Y. Mori and S. Yamaguchi.
Artif Organs, Vol. 20, No.11 (1996).
PMID: 8908335
Mebiol® Gel Non-Cell Culture Applications
Protein Crystallization Scaffold
see Sugiyama, et al., Cryst. Growth Des., 2013, 13 (5), pp 1899–1904
Scaffold for DNA Electrophoresis and Recovery
Separation and recovery of DNA fragments by electrophoresis through a thermoreversible hydrogel composed of poly(ethylene oxide) and poly(propylene oxide).
Yoshioka H, Mori Y, Shimizu M.
Anal Biochem. 2003 Dec 15;323(2):218-23.
PMID: 14656528
Cell sorting switch
On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow
Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu.
Anal. Chem., 78, 695-701 (2006)
PMID: 16448041
Cell sorting switch
On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow.
Anal Chem. 2006 Feb 1;78(3):695-701.
Shirasaki Y, Tanaka J, Makazu H, Tashiro K, Shoji S, Tsukita S, Funatsu T.
PMID: 16448041
Cell sorting switch
Microfluidic cell sorter with flow switching triggered by a sol–gel transition of a thermo-reversible gelation polymer.
Kazuto Ozaki, Hirokazu Sugino, Yoshitaka Shirasaki, Tokihiko Aoki, Takahiro Arakawa, Takashi Funatsu, Shuichi Shoji
Sensors and Actuators B: Chemical, Volume 150, Issue 1, 21 September 2010, Pages 449-455
http://www.sciencedirect.com/science/article/pii/S0925400510006143
DNA molecular Sorting
Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol–gel transition
Mai Haneoka, Yoshitaka Shirasaki, Hirokazu Sugino, Tokihiko Aoki, Takahiro Arakawa, Kazuto Ozaki, Dong Hyun Yoon, Noriyuki Ishii, Ryo Iizuka, Shuichi Shoji, Takashi Funatsu
Sensors and Actuators B: Chemical, Volume 159, Issue 1, 28 November 2011, Pages 314-320
http://www.sciencedirect.com/science/article/pii/S0925400511005521
Drug delivery
J Neurooncol. 2006 Mar;77(1):9-15.
Novel drug delivery system using thermoreversible gelation polymer for malignant glioma.
Arai T, Joki T, Akiyama M, Agawa M, Mori Y, Yoshioka H, Abe T.
J Neurooncol. 2006 Mar;77(1):9-15.
PMID: 16292493
Drug delivery
Novel local drug delivery system using thermoreversible gel in combination with polymeric microspheres or liposomes.
Arai T, Benny O, Joki T, Menon LG, Machluf M, Abe T, Carroll RS, Black PM.
Anticancer Res. 2010 Apr;30(4):1057-64.
PMID: 20530409
Cell Culture for drug screening
Alternatives to Animal Testing and Experimentation
Wakui et al. in vitro Thermoreversible Gel Disc Quantitative Assay of Rat Angiogenesis.
AATEX 16(2), 59-65, 2011
https://www.jstage.jst.go.jp/article/aatex/16/2/16_2_59/_pdf
Mebiol® Gel Physical Properties
Physical Properties
A synthetic hydrogel with thermoreversible gelation. II. : Effect of added salts.
H. Yoshioka, M. Mikami, Y. Mori, and E. Tsuchida.
J. Macromol. Sci., A31(1), 121-125 (1994).
http://www.tandfonline.com/doi/abs/10.1080/10601329409349723#.UvFzqtKE1NQ
Physical Properties
Thermoreversible gelation on heating and on cooling of an aqueous gelatin-poly(N-isopropylacrylamide) conjugate. ,
H. Yoshioka, Y. Mori, S. Tsukikawa, and S. Kubota,
Polym. Adv. Tech., 9, 155-158 (1998).
http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1099-1581(199802)9:2%3C155::AID-PAT750%3E3.0.CO;2-G/abstract
Physical Properties
A Synthetic hydrogel with thermoreversible gelation. I. Preparation and rheological properties , H. Yoshioka, M. Mikami and Y. Mori.
J.M.S- Pure Appl. Chem., A31(1), pp. 113-120 (1994).
http://www.tandfonline.com/doi/abs/10.1080/10601329409349722#.UvFK1dKE1NQ
Physical Properties
Preparation of Poly (N-Isopropylacrylamide)-b-Poly(Ethylene Glycol) and Calorimetric Analysis of its Aqueous Solution ,
H. Yoshioka, M. Mikami and Y. Mori,
J.M.S- Pure Appl. Chem., A31(1), pp. 109-112 (1994).
http://www.tandfonline.com/doi/abs/10.1080/10601329409349721#.UvFLB9KE1NQ
Physical Properties
A synthetic hydrogel with thermoreversible gelation. III. : An NMR study of the Sol-Gel transition.
H. Yoshioka, Y. Mori and James A. Cushman.
Polym. Adv. Tech., 5, pp. 122-127 (1994).
http://onlinelibrary.wiley.com/doi/10.1002/pat.1994.220050207/abstract