LipoTrust™ Dashboard 

Transfection Reagents

      

Product Lineup / Ordering Information

LipoTrust™ EX Series

Catalog Number (click to view datasheet)	Product Name	Size
CSR-LEG-01-EX	LipoTrust EX Gene Plasmid DNA transfection reagent	1 ml
CSR-LEO-01-EX	LipoTrust EX Oligo for In Vitro short oligonucleotides transfection reagent such as siRNA, antisense DNA, miRNA	1 ml

LipoTrust™ Series

Catalog Number (click to view datasheet)	Product Name	Size
CSR-LCH-01-EX	LipoTrust CH shows remarkably high gene expression activity in the serum-free medium, and is available for transfection into non-adherent type cells.	1 ml
CSR-LPE-01-EX	LipoTrust PE shows gene expression activity of a certain extent with / without serum.	1 ml
CSR-LSR-01-EX	LipoTrust SR < temporarily unavailable > shows high gene expression activity with many kinds of cells even in the presence of serum.	1 ml
CSR-LTS-01-EX	LipoTrust Set < temporarily unavailable > a set that contains the above three LipoTrust products	1 Set

 

LipoTrust™ EX Oligo

• short oligonucleotides transfection reagent such as siRNA, antisense DNA, miRNA
  
• It has a higher knockdown effect than other competing products.
• Efficient transfection of short oligonucleotides at low concentrations.
• Transfection can be performed in the presence of serum.
• By condensing with a nucleic acid solution according to your preference, you can expect a higher level of transfection.
• *Lyophilized product: for 1ml
• *A 24-well plate can be used for approximately 500 reactions
  

  Successful examples of cell transfection

  Cell type	Transfection Efficiency Level
  HeLa Cell	Excellent
  CHO Cell	Excellent
  KB Cell	Excellent
  HT1080 Cell	Good
  3T3-L1 Cell	Good
  COS-1 Cell	Excellent
  Caco-2 Cell	Good

   

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LipoTrust™ EX Gene

•  Plasmid DNA transfection reagent
• Excellent transfection efficiency.
• Low cytotoxicity, allowing transfection with healthy cells.
• Transfection can be performed in the presence of serum.
• No need to change medium after transfection.
• *Lyophilized product: for 1ml
• *A 24-well plate can be used for approximately 500 reactions.
  

  Successful examples of cell transfection

  Cell type	Transfection Efficiency Level
  HeLa Cell	Excellent
  HT1080 Cell	Excellent
  NB1RGB Cell	Good
  293T Cell	Excellent
  COS-1 Cell	Excellent

  
  

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LipoTrust™ Series

This is cationic liposome which consists of three highly effective transfection formulations strictly selected from more than three hundreds. What makes them unique is that no conversion to serum-free medium is required at the transfection. And they show high gene expression activity both in vitro and in vitro.

LipoTrust™ PE

• shows constant gene expression activity regardless of the presence or absence of serum.

LipoTrust™ CH

• Shows extremely high gene expression activity in serum-free medium, and can also be used to introduce genes into blood cells.
  

LipoTrust Set  < temporarily unavailable >

• LipoTrust SR, PE and CH 
  

  Successful examples of cell transfection

  Cell type	Transfection Efficiency	Cell type	Transfection Efficiency
  293	Excellent	HRA	Excellent
  3T3	Good	Jurkat	Good
  A431	Good	MDCK	Good
  A549	Good	ｍEIIL	Excellent
  B95a	Good	Morris	Excellent
  C33A	Good	Neuro-2A	Excellent
  COS-1	Good	PC3	Good
  COS-7	Excellent	RBL-2H3	Excellent
  ES	Excellent	Walker-256	Excellent
  H322	Good	Mouse Fibroblast	Excellent
  H520	Good	Mouse Granule Cell (Neuron)	Excellent
  HeLa	Excellent	Calf Vascular Endothelial Cell	Good
  HepG2	Good	Dorsal Root Ganglion	Excellent

  
  
  

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References

Rai K, Takigawa N, Ito S, Kashihara H, Ichihara E, Yasuda T et al.
Liposomal Delivery of MicroRNA-7-Expressing Plasmid Overcomes Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor-Resistance in Lung Cancer
Cells. Mol Cancer Ther. 2011; 10(9):1720-7

Efficient delivery of liposome-mediated MGMT-siRNA reinforces the cytotoxity of temozolomide in GBM-initiating cells T Kato, A Natsume et al.
Gene Therapy, 1-9 (2010)

Knocking down barriers:advances in siRNA deliveryKathryn A. Whitehead, Robert Langer and Daniel G. Anderson Nature Review siRNA delivery 129-138

Poster #1396: MicroRNA-7 downregulates epidermal growth factor receptor in both gefitinib-sensitive and resistant lung carcinoma cells: presented by Kammei Rai et al 100th AACR in April 19, 2009.

Resolution of liver cirrhosis using vitamin A-coupled lipos Development of novel cationic liposomes for efficient gene transfer into peritoneal disseminated tumor.
Hum. Gene Ther .,10,947-955 (1999)

Development of novel cationic liposomes for efficient gene transfer into peritoneal disseminated tumor.
Hum. Gene Ther.,10,947-955 (1999)

Gene delivery using liposome technology.
J. Control.Release , 62,269-277(1999)

A new cationic liposome for efficient gene delivery with serum into cultured human cells: A quantitative analysis using two independent fluorescent probes.
Biochim.Biophys.Acta ,1467,419-430(2000)

Characteristics and biodistribution of cationic liposomes and their complexes.
J.Control.Release ,69,139-148(2000)

Phorbol ester-potentiated liposomal transfection to monocytic PLB-985 cells.
J.Biochem .,128,989-998(2000)

Quantitative analysis of correlation between number of nuclear plasmids and gene expression activity after transfection with cationic liposomes.
Pharm.Res .,19,377-381(2002)

PU.1 is dominant and HAF-1 supplementary for activation of the gp91 phox promoter in human monocytic PLB-985 cells.
J.Biochem .,131.533-540(2002)

Norepinephrine enhances fibrosis mediated by TGF- in cardiac fibroblast.
Hypertension ,40,148-154(2002)

“Liposomal formulations as nanotechnology: past, present, and future (Part 2)”
PHARM TECH JAPAN, 19, 419-433 (2003)

“Application of stealth liposomes to cancer chemotherapy/gene therapy”
Biotherapy, 18, 353-360 (2004)