Kohjin Bio (KOJ) Products : COS Banker Series


	COS banker is a safe cryopreservation solution for cultured cells that does not contain any serum or bovine-derived compounds. COS banker II is a lymphocyte cryopreservation solution developed for the preservation of adopted immune lymphocytes.

COS Banker
COS Banker is a chemically defined cryopreservative which contains neither serum components nor other proteins. Therefore, this
cryopreservative is used when cells adapted to serum-free culture conditions are frozen for their preservation.
Monolayer cells, lymphoid cells, and transformed cells are cryopreserved without remarkable lose of viability. These cells include Vero cells, MDCK cells, CHO cells, HeLa cells and other cell lines in addition to activated lymphocytes.

COS Banker Ⅱ
COS Banker II is developed for cryopreservation of lymphoid cells.
To improve the viability after cell-thawing, COS Banker II is supplemented with human albumin. Cryopreservation of activated human lymphocytes is recommended. Other cells cultured in a suspension form are also cryopreserved in COS Banker II. These cells include Daudi cells, Raji cells, K562 cells, Molt-4F cells, and CTLL-2 cells.

Cos Banker Series Flyer: PDF Download [1271 kb] [10189]
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[Notice] Standard change for COS Banker and COS Banker II

The standards of our product "COS banker (COS-CFM01), COS banker II(COS-CFM02)" has been changed. This standard change will not affect product performance.
■ Summary of changes
Change from pH 6.8 ± 0.2 to 7.1 ± 0.5 The pH may rise during storage of the product, and as a result of verification, it was confirmed that the performance of the product can be guaranteed by the standard after the change.

■ Change time
From Products manufactured after March 2015

Features

Frozen cells
It is also possible to cryopreserve iPS cells. Click here for data NEW
It can be used as it is without diluting it.
Excellent cell viability after thawing.
"COS banker" does not contain animal serum or other animal-derived ingredients. * 1
A glass bottle is used to prevent the PET container from being corroded by the contained DMSO.

* 1 "COS banker II" contains GMP grade human serum albumin as a stabilizer.

Protocol

Centrifuge cells in the logarithmic growth phase * 2 at 1200 rpm / 5 minutes and collect about 5 x 10 5 to 1 x 10 7 cells.
Add 1 mL of this product to pellet cells, suspend, place in a cryopreservation tube, freeze at -80 ° C, and store.
When storing in liquid nitrogen, store cells that have been frozen at -80 ° C for at least 1 day in advance.
Thaw immediately in a 37 ° C constant temperature water tank.
* 2 It is important that the cells to be cryopreserved are in the logarithmic growth phase in order to improve the survival rate after thawing.

Specifications

Product name	COS banker	COS banker II
Product number	KOJ-COS-CFM01	KOJ-COS-CFM02
Packaging	120 mL	120 mL
specification	DMSO content (content not disclosed) Serum-free	DMSO-containing (content not disclosed) Human serum albumin-containing serum-free
Product storage method	Store in a cool and dark place (2-8 ° C)	Store in a cool and dark place (2-8 ° C)
date of expiry	2 years after manufactured date	2 years after manufactured date

Quality Check

Inspection item	Test method	Standard
Asepticity	TGC, SCD and culture method (Japan Bureau)	Does not allow growth
Endotoxin	Kinetic colorimetric method (Japan Bureau)	0.3 EU / mL or less
Cell preservation	Cell survival on rethaw after 7 days of freezing	Equivalent to control preservation solution

Example of usage

COS banker

COS banker II

iPS cells (Dotcom)
Daudi (human barkit lymphoma), HeLa S3 (human cervical cancer)
K562 (human chronic myeloid leukemia), Raji (human barkit lymphoma)
MOLT-4 (human acute lymphoblastic leukemia: T-cells
CTLL-2 (mouse T: IL-2 dependent), NIH / 3T3 (mouse fetal)
PC-12 (rat adrenal brown cell tumor), CHO-K1 (Chinese hamster ovary),
vero cells (African green monkey ) Kidney-derived cell line),
MDCK cell (canine kidney tubule epithelial cell-derived cell line)
MCF-7 (human breast cancer), HepG2 (human liver cancer),
P3U1 (mouse myeloma), fibroblasts,
epidermal keratinized cells, Vascular endothelial cells, hybrid domas, SF9 (insect cells)

Activated lymphocytes derived from human peripheral blood,
Daudi cells, Raji cells, K652 cells,
Molt-4F cells, CTLL-2 cells, MCF-7 (human breast cancer),
HepG2 (human liver cancer), P3U1 (mouse myeloma),
fibers Blast cells, epidermal keratinized cells, vascular endothelial cells,
hybridoma, CHO cells (Chinese hamster ovary-derived cell line),
Vero cells (African green monkey kidney-derived cell line),
MDCK cells (canine kidney tubule epithelial cell-derived cell line), HeLa (human cervical cancer), SF9 (insect cells)

iPS cells

[Storage procedure (slow freezing method)]

Collect cells using CTK solution, etc.
Centrifuge (1000 rpm, 5 minutes) to remove supernatant
Add Cos Banker and suspend cells
Dispense into a cryovial
Place the cryovial in a bicelle cryopreservation container.
Place in a -80 ° C freezer
The next day, move the cryovial to liquid nitrogen


Figure 1 Results of cryopreservation of iPS cells (Dotcom) Left: Day 2 after thawing, Right: Day 6 after thawing

Human lymphocytes

Fig. 2 (Left figure) Comparison of cell viability comparison of cell cryopreservation solution 0.5 mL (live cell concentration 3 × 10 ⁶ cells / mL) was added to a cryotube and stored at -80 ° C. After 1 week / 1 month / 2 months, the cells were thawed (incubated in a constant temperature water bath at 37 ° C. for 90 seconds, and the remaining frozen cells were thawed by shaking), and the survival rate was measured.　Gray: 1 week later, Blue: 1 month later, Black: 2 months later

Fig. 3 (Right figure) Cell proliferation after　thawing Lymphocytes that have been cryopreserved for 1 week are thawed , adjusted in each medium to a viable cell concentration of 20 × 10 ⁴ cells / mL, and incubator (37 ° C, 5%). It was cultured in CO ₂ ). The number of living cells was measured on the 5th and 6th days.