Serum-Free Media for Mesenchymal Stem Cells

KBM ADSC-5

Characteristics
●Serum-free, Animal Origin Free(AOF), Chemically Defined culture media developed for human mesenchymal stem cells.
●Long-term passaging of MSC in undifferentiated state is possible (confirmed for adipose-derived and umbilical cord-derived cells).
●High proliferative potential
●No need to add supplements
●Phenol red free
●No coating required for culture containers  (confirmed for major products in Japan and Overseas).

Cell culture example Using KBM ADSC-2+2% serum substitute, cell proliferation tests of adipose-derived stem cells cultured to P2, confirmation of cell surface markers after five passages and differentiation induction tests were carried out. It was also confirmed that the proliferation performance was almost equivalent in umbilical cord-derived stem cells.

                          Fig.1:Growth curve                                         Fig.2:Variation of PDT                      Fig.3:Cell morphology at P5          Table.1: Ratio of cell surface markers  at P5 

Fig.4: Differentiation potential confirmation test of MSC at P6  a) Adipose cells (Oil red O stain) b) Osteoblastic cells (Alizarin Red S stain) c) Chondrocytes (Alcian Blue stain)	Fig 5: Culture test data  of Umbilical cord-derived MSC a) Cell morphology at P5 b) PDL at passage