Anti HEV (IgG/IgM), EIA
| Catalog Number | Product Name | Reactivity | Size |
| IIM-1Z23 | Anti HEV (IgG/IgM), EIA | Human | 1 x 96 rxns |
| This kit is a detection kit of IgG anti-HEV antibody or IgM anti-HEV antibody in human serum. IgG anti-HEV antibody is detected in sera of patients infected by hepatitis E virus (HEV) in the past, and IgM anti-HEV antibody is detected during the acute stage of infection with HEV. |
 |
||
Background
IgG anti-HEV antibody is detected in sera of patients infected by hepatitis E virus (HEV) in the past, and IgM anti-HEV antibody is detected during the acute stage of infection with HEV.
The detection system of this kit is based on the enzyme immuno assay (EIA) and is made of 2 steps: the antigen-antibody reaction and the enzyme coloring reaction. The first antigen-antibody reaction takes place between the HEV antigen coated on the microplate and the anti-HEV antibody in samples while the second reaction takes place between the IgG/IgM anti-HEV antibody bound to the antigen coated on the microplate and the antibody labeled with enzyme (horseradish peroxidase labeled antibody). When the IgG/IgM anti-HEV antibody is present in samples, the first and the second reactions take place. Then, absorbance by color proportional to the amount of the IgG/IgM anti-HEV antibody in samples developed by enzyme reaction is measured.
Application
Detection of IgG anti-HEV antibody or IgM anti-HEV antibody in human serum
Assay principle
IgG anti-HEV antibody is detected in sera of patients infected by hepatitis E virus (HEV) in the past, and IgM anti-HEV antibody is detected during the acute stage of infection with HEV. enzyme immunoassay (EIA) and is made up of 2 steps of the antigen-antibody reaction and the enzyme coloring reaction. The first antigen-antibody reaction takes place between the HEV antigen coated on the microplate and the anti-HEV antibody in samples and the second reaction between the IgG/IgM anti-HEV antibody bound to the antigen coated on the microplate and the antibody labeled with enzyme (horseradish peroxidase labeled antibody). the second reactions take place and absorbance by color proportional to the amount of the IgG/IgM anti-HEV antibody in samples developed by enzyme reaction is measured.
Materials required but not provided
1) Micropipettes, 10 µL, 100 µL, 200 µL, and 1,000 µL
2) A measuring cylinder, 1L
3) An aspirator and a polyethylene washing bottle, or a microplate washer
4) A dark box (A light tight cupboard or a drawer will do)
5) A dual-wavelength microplate reader (main wavelength 440 ~ 460)nm, sub wavelength 620 nm or longer)
References
1) Mikhail S, Balayan MD: Type E Hepatitis: State of the Art. Int J Infect Dis 2 (2): 113-120, 1997.
2) Mizuo H, Suzuki K, Takikawa Y, et al: Polyphyletic strains of hepatitis E virus are responsible for sporadic cases of acute hepatitis in Japan. J Clin Microbiol 40: 3209-3218, 2002.
3) Takahashi M, Nishizawa T, Miyajima H, et al: Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus. J Gen Virol 84: 851-862, 2003
4) Takahashi M, Kusakai S, Mizuo H, et al: Simultaneous detection of immunoglobulin A (IgA) and IgM antibodies against hepatitis E virus (HEV) is highly specific for diagnosis of acute HEV infection. J Clin Microbiol 43: 49-56 , 2005