Affinity Resin for Antibody Purification

 

 Alkali-resistant mutants of protein A, protein L, and protein G, which are ligand proteins for antibody purification

 

• Bipo Resin Protein A (Alkaline Resistance)
• Bipo Resin Protein A (Weak Acid)
• Bipo Resin Protein L (Alkaline Resistance)
• Bipo Resin Protein G (Alkaline Resistance)

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Catalog Number	Product Name	Size	Datasheet
PRX-AAR-025	Bipo Resin Protein A (Alkaline Resistance)	25 ml	Datasheet
PRX-AWA-025	Bipo Resin Protein A (Weak Acid)	25 ml
PRX-GAR-025	Bipo Resin Protein G (Alkaline Resistance)	25 ml
PRX-LAR-025	Bipo Resin Protein L (Alkaline Resistance)	25 ml

Overview

Protein A (Staphylococcus aureus), Protein L (Finegoldia magna), Protein G (group G streptococci) are known as affinity molecules to antibodies.
We developed affinity resin for antibody purification using these affinity molecules.

Features

• Since a synthetic polymer is used for the matrix, non-specific adsorption is low and high pressure resistance can be expected.
• It has better cost performance than conventional antibody purification resins.
• Four types of resin are available. Please choose according to your needs.
• Alkali resistant protein A resin, alkali resistant protein G resin (Catalog No.: PRX-AAR-025, PRX-GAR-025)
  It has high alkali resistance and can be used repeatedly.

• Weak acid eluting protein A resin (code: PRX-AWA-025)
  Possible to elute with weak acidity. The risk of antibody deactivation and aggregation can be avoided.

• Alkali resistant protein L resin (code: PRX-LAR-025)
  It has high alkali resistance and can be used repeatedly.
  Can be used to purify antibodies containing kappa light chains, such as small antibody scFv.

Application Data

1. Marker 2. IgG from Bipo Resin Protein A (Alkaline Resistance) 3. IgG from Bipo Resin Protein A (Weak Acid) 4. IgG from competitor ProteinA Resin 5. marker 6. IgG from Bipo Resin Protein L (Alkaline Resistance) 7. IgG from competitor ProteinL Resin 8. scFv from Bipo Resin Protein L (Alkaline Resistance) 9. scFv from competitor ProteinL Resin 10. marker 11. IgG from Bipo Resin Protein G (AlkalineResistance) 12. IgG from competitor ProteinG Resin 13. marker

 scFv purification by Bipo Resin Protein L

*Bipo Resin Protein L scFv was purified using
Approximately 3.5 times more scFv than conventional products

Effect of alkaline washing on antibody binding capacity

Bipo Resin Protein A (Alkaline Resistance)

Bipo Resin Protein L (Alkaline Resistance)

Bipo Resin Protein G (Alkaline Resistance)

Elution efficiency under weak acidity (pH4.5)

Bipo Resin Protein A (Weak Acid)